Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients
The current study collected 157 clinical samples from Iraqi patients suffering from urinary tract infections at various Baghdad hospitals, including Medical City/Educational Laboratories, Baghdad Teaching Hospital, Imamin Al-Kadhimiya Hospital, and the Central Children's Hospital in Baghdad, f...
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University of Baghdad
2025-07-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
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| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3652 |
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| author | Zahraa Jamaal Al -Deen M. Shafeeq A.ALateef AL-Badri Souad Khalil Ibrahim |
| author_facet | Zahraa Jamaal Al -Deen M. Shafeeq A.ALateef AL-Badri Souad Khalil Ibrahim |
| author_sort | Zahraa Jamaal Al -Deen M. Shafeeq A.ALateef AL-Badri |
| collection | DOAJ |
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The current study collected 157 clinical samples from Iraqi patients suffering from urinary tract infections at various Baghdad hospitals, including Medical City/Educational Laboratories, Baghdad Teaching Hospital, Imamin Al-Kadhimiya Hospital, and the Central Children's Hospital in Baghdad, from 27 November 2022 to 11 January 2023. We identified and tested the formation of biofilms, which led to the detection of two genes, algD and TspE4.C2. Vitek 2 confirmed the final diagnosis after a biochemical and microscopic examination of 11 selected E. coli isolates. The Congo red method has detected the bacterial vulnerability to form biofilms. he findings of this study showed that all selected bacterial isolates formed the biofilm with a moderate degree (11 (100%)). The polymerase chain reaction (PCR) identified the algD and TspE4.C2 genes, demonstrating their significant role in biofilm production. The polymerase chain reaction results revealed the presence of the algD gene in 9 (81.8%) out of 11 isolates, while the TspE4.C2 gene was present in 10 (90.9%) isolates
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| format | Article |
| id | doaj-art-0f5f760d13904a7a87bb4705af5976ea |
| institution | DOAJ |
| issn | 1609-4042 2521-3407 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj-art-0f5f760d13904a7a87bb4705af5976ea2025-08-20T03:12:31ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072025-07-0138310.30526/38.3.3652Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection PatientsZahraa Jamaal Al -Deen M. Shafeeq A.ALateef AL-Badri 0https://orcid.org/0009-0001-5412-1137Souad Khalil Ibrahim1https://orcid.org/0009-0000-1526-1682Department of Biology, Collage of Education for Pure Science (Ibn Al-Haitham) ,University of Baghdad/Iraq.Department of Biology, College of Education for Pure Science (Ibn Al- Haitham), University of Baghdad, Baghdad, Iraq. The current study collected 157 clinical samples from Iraqi patients suffering from urinary tract infections at various Baghdad hospitals, including Medical City/Educational Laboratories, Baghdad Teaching Hospital, Imamin Al-Kadhimiya Hospital, and the Central Children's Hospital in Baghdad, from 27 November 2022 to 11 January 2023. We identified and tested the formation of biofilms, which led to the detection of two genes, algD and TspE4.C2. Vitek 2 confirmed the final diagnosis after a biochemical and microscopic examination of 11 selected E. coli isolates. The Congo red method has detected the bacterial vulnerability to form biofilms. he findings of this study showed that all selected bacterial isolates formed the biofilm with a moderate degree (11 (100%)). The polymerase chain reaction (PCR) identified the algD and TspE4.C2 genes, demonstrating their significant role in biofilm production. The polymerase chain reaction results revealed the presence of the algD gene in 9 (81.8%) out of 11 isolates, while the TspE4.C2 gene was present in 10 (90.9%) isolates https://jih.uobaghdad.edu.iq/index.php/j/article/view/3652Escherichia coli, urinary tract infection, Molecular characterization |
| spellingShingle | Zahraa Jamaal Al -Deen M. Shafeeq A.ALateef AL-Badri Souad Khalil Ibrahim Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients Ibn Al-Haitham Journal for Pure and Applied Sciences Escherichia coli, urinary tract infection, Molecular characterization |
| title | Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients |
| title_full | Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients |
| title_fullStr | Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients |
| title_full_unstemmed | Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients |
| title_short | Molecular Characterization of algD and TspE4.C2 Genes in Escherichia coli Isolated from Urinary Tract Infection Patients |
| title_sort | molecular characterization of algd and tspe4 c2 genes in escherichia coli isolated from urinary tract infection patients |
| topic | Escherichia coli, urinary tract infection, Molecular characterization |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3652 |
| work_keys_str_mv | AT zahraajamaalaldeenmshafeeqaalateefalbadri molecularcharacterizationofalgdandtspe4c2genesinescherichiacoliisolatedfromurinarytractinfectionpatients AT souadkhalilibrahim molecularcharacterizationofalgdandtspe4c2genesinescherichiacoliisolatedfromurinarytractinfectionpatients |