Molecular subtyping of hypertensive disorders of pregnancy

Abstract Hypertensive disorders of pregnancy (HDP), including preeclampsia, affect 1 in 6 pregnancies, are major contributors to maternal morbidity and mortality, yet lack precision medicine strategies. Analyzing transcriptomic data from a prospectively-collected diverse cohort (n = 9102), this stud...

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Main Authors: Michal A. Elovitz, Elaine P. S. Gee, Nathaniel Delaney-Busch, Alison B. Moe, Mitsu Reddy, Arkady Khodursky, Johnny La, Ilma Abbas, Kay Mekaru, Hunter Collins, Farooq Siddiqui, Rory Nolan, Rupsa C. Boelig, Daniel G. Kiefer, Pamela M. Simmons, George R. Saade, Antonio Saad, Ebony B. Carter, Thomas F. McElrath, Stephen R. Quake, Mark A. DePristo, Carrie Haverty, Manfred Lee, Eugeni Namsaraev, Vincenzo Berghella, Ai-ris Y. Collier, Antonia I. Frolova, Esther Park-Hwang, Luis D. Pacheco, Elizabeth F. Sutton, Maneesh Jain, Kara Rood, William A. Grobman, Joseph R. Biggio, Cynthia Gyamfi-Bannerman, Arun Jeyabalan, Morten Rasmussen
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-58157-y
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author Michal A. Elovitz
Elaine P. S. Gee
Nathaniel Delaney-Busch
Alison B. Moe
Mitsu Reddy
Arkady Khodursky
Johnny La
Ilma Abbas
Kay Mekaru
Hunter Collins
Farooq Siddiqui
Rory Nolan
Rupsa C. Boelig
Daniel G. Kiefer
Pamela M. Simmons
George R. Saade
Antonio Saad
Ebony B. Carter
Thomas F. McElrath
Stephen R. Quake
Mark A. DePristo
Carrie Haverty
Manfred Lee
Eugeni Namsaraev
Vincenzo Berghella
Ai-ris Y. Collier
Antonia I. Frolova
Esther Park-Hwang
Luis D. Pacheco
Elizabeth F. Sutton
Maneesh Jain
Kara Rood
William A. Grobman
Joseph R. Biggio
Cynthia Gyamfi-Bannerman
Arun Jeyabalan
Morten Rasmussen
author_facet Michal A. Elovitz
Elaine P. S. Gee
Nathaniel Delaney-Busch
Alison B. Moe
Mitsu Reddy
Arkady Khodursky
Johnny La
Ilma Abbas
Kay Mekaru
Hunter Collins
Farooq Siddiqui
Rory Nolan
Rupsa C. Boelig
Daniel G. Kiefer
Pamela M. Simmons
George R. Saade
Antonio Saad
Ebony B. Carter
Thomas F. McElrath
Stephen R. Quake
Mark A. DePristo
Carrie Haverty
Manfred Lee
Eugeni Namsaraev
Vincenzo Berghella
Ai-ris Y. Collier
Antonia I. Frolova
Esther Park-Hwang
Luis D. Pacheco
Elizabeth F. Sutton
Maneesh Jain
Kara Rood
William A. Grobman
Joseph R. Biggio
Cynthia Gyamfi-Bannerman
Arun Jeyabalan
Morten Rasmussen
author_sort Michal A. Elovitz
collection DOAJ
description Abstract Hypertensive disorders of pregnancy (HDP), including preeclampsia, affect 1 in 6 pregnancies, are major contributors to maternal morbidity and mortality, yet lack precision medicine strategies. Analyzing transcriptomic data from a prospectively-collected diverse cohort (n = 9102), this study reveals distinct RNA subtypes in maternal blood, reclassifying clinical HDP phenotypes like early/late-onset preeclampsia. The placental gene PAPPA2 strongly predicts the most severe forms of preeclampsia in individuals without pre-existing high risk factors, months before symptoms, and its overexpression correlates with earlier delivery in a dose-dependent manner. Further, molecular subtypes characterized by immune genes are upregulated in less severe forms of HDP. These results reclassify HDP clinical phenotypes into two distinct molecular subtypes, placental-associated or immune-associated. Validation performance for placental-associated HDP yields an AUC of 0.88 in the advanced maternal age population without pre-existing high risk factors. Molecular subtypes create new opportunities to apply precision-based medicine in maternal health.
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spelling doaj-art-0f5e6ea813804849891f86fa595e0cf22025-08-20T02:12:06ZengNature PortfolioNature Communications2041-17232025-04-0116111410.1038/s41467-025-58157-yMolecular subtyping of hypertensive disorders of pregnancyMichal A. Elovitz0Elaine P. S. Gee1Nathaniel Delaney-Busch2Alison B. Moe3Mitsu Reddy4Arkady Khodursky5Johnny La6Ilma Abbas7Kay Mekaru8Hunter Collins9Farooq Siddiqui10Rory Nolan11Rupsa C. Boelig12Daniel G. Kiefer13Pamela M. Simmons14George R. Saade15Antonio Saad16Ebony B. Carter17Thomas F. McElrath18Stephen R. Quake19Mark A. DePristo20Carrie Haverty21Manfred Lee22Eugeni Namsaraev23Vincenzo Berghella24Ai-ris Y. Collier25Antonia I. Frolova26Esther Park-Hwang27Luis D. Pacheco28Elizabeth F. Sutton29Maneesh Jain30Kara Rood31William A. Grobman32Joseph R. Biggio33Cynthia Gyamfi-Bannerman34Arun Jeyabalan35Morten Rasmussen36Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Sidney Kimmel Medical College, Thomas Jefferson UniversityWomen’s Care FloridaWoman’s HospitalEastern Virginia Medical SchoolInova HealthUniversity of North Carolina at Chapel HillMirvie Inc.Department of Bioengineering, Stanford UniversityBigHat Biosciences Inc.Mirvie Inc.Mirvie Inc.Mirvie Inc.Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Sidney Kimmel Medical College, Thomas Jefferson UniversityDepartment of Obstetrics and Gynecology, Beth Israel Deaconess Medical CenterWashington University School of MedicineMultiCare Health SystemUniverity of Texas Medical BranchWoman’s HospitalMirvie Inc.The Ohio State UniversityThe Ohio State UniversityOchsner HealthUniversity of California San DiegoUniversity of PittsburghMirvie Inc.Abstract Hypertensive disorders of pregnancy (HDP), including preeclampsia, affect 1 in 6 pregnancies, are major contributors to maternal morbidity and mortality, yet lack precision medicine strategies. Analyzing transcriptomic data from a prospectively-collected diverse cohort (n = 9102), this study reveals distinct RNA subtypes in maternal blood, reclassifying clinical HDP phenotypes like early/late-onset preeclampsia. The placental gene PAPPA2 strongly predicts the most severe forms of preeclampsia in individuals without pre-existing high risk factors, months before symptoms, and its overexpression correlates with earlier delivery in a dose-dependent manner. Further, molecular subtypes characterized by immune genes are upregulated in less severe forms of HDP. These results reclassify HDP clinical phenotypes into two distinct molecular subtypes, placental-associated or immune-associated. Validation performance for placental-associated HDP yields an AUC of 0.88 in the advanced maternal age population without pre-existing high risk factors. Molecular subtypes create new opportunities to apply precision-based medicine in maternal health.https://doi.org/10.1038/s41467-025-58157-y
spellingShingle Michal A. Elovitz
Elaine P. S. Gee
Nathaniel Delaney-Busch
Alison B. Moe
Mitsu Reddy
Arkady Khodursky
Johnny La
Ilma Abbas
Kay Mekaru
Hunter Collins
Farooq Siddiqui
Rory Nolan
Rupsa C. Boelig
Daniel G. Kiefer
Pamela M. Simmons
George R. Saade
Antonio Saad
Ebony B. Carter
Thomas F. McElrath
Stephen R. Quake
Mark A. DePristo
Carrie Haverty
Manfred Lee
Eugeni Namsaraev
Vincenzo Berghella
Ai-ris Y. Collier
Antonia I. Frolova
Esther Park-Hwang
Luis D. Pacheco
Elizabeth F. Sutton
Maneesh Jain
Kara Rood
William A. Grobman
Joseph R. Biggio
Cynthia Gyamfi-Bannerman
Arun Jeyabalan
Morten Rasmussen
Molecular subtyping of hypertensive disorders of pregnancy
Nature Communications
title Molecular subtyping of hypertensive disorders of pregnancy
title_full Molecular subtyping of hypertensive disorders of pregnancy
title_fullStr Molecular subtyping of hypertensive disorders of pregnancy
title_full_unstemmed Molecular subtyping of hypertensive disorders of pregnancy
title_short Molecular subtyping of hypertensive disorders of pregnancy
title_sort molecular subtyping of hypertensive disorders of pregnancy
url https://doi.org/10.1038/s41467-025-58157-y
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