Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice

Blood stasis syndromes (BSSs) are closely related to the occurrence and development of tumors, although the mechanism is still unclear. This study was aimed at exploring the effect and mechanism underlying different BSSs on tumor growth and metastasis. We established four BSS mouse models bred with...

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Main Authors: Lu Jin, Biqiang Tang, Xia Liu, Weiye Mao, Linying Xia, Yueguang Du, Bing Ji, Qiyang Shou, Huiying Fu
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2022/7222638
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author Lu Jin
Biqiang Tang
Xia Liu
Weiye Mao
Linying Xia
Yueguang Du
Bing Ji
Qiyang Shou
Huiying Fu
author_facet Lu Jin
Biqiang Tang
Xia Liu
Weiye Mao
Linying Xia
Yueguang Du
Bing Ji
Qiyang Shou
Huiying Fu
author_sort Lu Jin
collection DOAJ
description Blood stasis syndromes (BSSs) are closely related to the occurrence and development of tumors, although the mechanism is still unclear. This study was aimed at exploring the effect and mechanism underlying different BSSs on tumor growth and metastasis. We established four BSS mouse models bred with breast cancer: qi deficiency and blood stasis (QDBS), cold coagulation blood stasis (CCBS), heat toxin and blood stasis (HTBS), and qi stagnation and blood stasis (QSBS). The results showed that microcirculation in the lower limb, abdominal wall, and tumor in situ decreased by varying degrees in the BSS groups. In addition, BSS promoted tumor growth and lung metastasis. The ratio of regulatory T cells in the tumor microenvironment was downregulated. Moreover, hypoxia-inducible factor 1-α, Wnt1, β-catenin, vascular endothelial growth factor, and Cyclin D1 levels increased in the tumors of BSS mice. In conclusion, BSS not only promoted the formation of a hypoxic and immunosuppressive microenvironment but also promoted the neovascularization.
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issn 2314-7156
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publisher Wiley
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series Journal of Immunology Research
spelling doaj-art-0f4dc4cbf72840a593d5605dbc8ef3dc2025-08-20T03:54:29ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/7222638Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in MiceLu Jin0Biqiang Tang1Xia Liu2Weiye Mao3Linying Xia4Yueguang Du5Bing Ji6Qiyang Shou7Huiying Fu8Second Clinical Medical SchoolSecond Clinical Medical SchoolSecond Clinical Medical SchoolSecond Clinical Medical SchoolSecond Clinical Medical SchoolSchool of Basic MedicineHuzhou Hospital of Traditional Chinese MedicineSecond Clinical Medical SchoolSecond Clinical Medical SchoolBlood stasis syndromes (BSSs) are closely related to the occurrence and development of tumors, although the mechanism is still unclear. This study was aimed at exploring the effect and mechanism underlying different BSSs on tumor growth and metastasis. We established four BSS mouse models bred with breast cancer: qi deficiency and blood stasis (QDBS), cold coagulation blood stasis (CCBS), heat toxin and blood stasis (HTBS), and qi stagnation and blood stasis (QSBS). The results showed that microcirculation in the lower limb, abdominal wall, and tumor in situ decreased by varying degrees in the BSS groups. In addition, BSS promoted tumor growth and lung metastasis. The ratio of regulatory T cells in the tumor microenvironment was downregulated. Moreover, hypoxia-inducible factor 1-α, Wnt1, β-catenin, vascular endothelial growth factor, and Cyclin D1 levels increased in the tumors of BSS mice. In conclusion, BSS not only promoted the formation of a hypoxic and immunosuppressive microenvironment but also promoted the neovascularization.http://dx.doi.org/10.1155/2022/7222638
spellingShingle Lu Jin
Biqiang Tang
Xia Liu
Weiye Mao
Linying Xia
Yueguang Du
Bing Ji
Qiyang Shou
Huiying Fu
Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
Journal of Immunology Research
title Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
title_full Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
title_fullStr Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
title_full_unstemmed Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
title_short Blood Stasis Syndrome Accelerates the Growth and Metastasis of Breast Cancer by Promoting Hypoxia and Immunosuppressive Microenvironment in Mice
title_sort blood stasis syndrome accelerates the growth and metastasis of breast cancer by promoting hypoxia and immunosuppressive microenvironment in mice
url http://dx.doi.org/10.1155/2022/7222638
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