Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study
Background and Objective: Bioceramic materials have revolutionized the field of endodontics by successfully transforming the outcomes of pulp therapies. Novel biomaterials are evolving by modifying the conventional mineral trioxide aggregate (MTA) to overcome their existing limitations, the major on...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1078_24 |
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| author | G. R. Ishwarya Dhevi Ponnudurai Arangannal J Jeevarathan |
| author_facet | G. R. Ishwarya Dhevi Ponnudurai Arangannal J Jeevarathan |
| author_sort | G. R. Ishwarya Dhevi |
| collection | DOAJ |
| description | Background and Objective:
Bioceramic materials have revolutionized the field of endodontics by successfully transforming the outcomes of pulp therapies. Novel biomaterials are evolving by modifying the conventional mineral trioxide aggregate (MTA) to overcome their existing limitations, the major ones being prolonged setting and cytotoxic radiopacifiers. Dental white portland cement (DWPC) is a novel formulated bioceramic material introduced as an enhanced MTA alternative. This in vitro study aims to investigate the cytotoxicity and odontogenic differentiation of novel formulation DWPC with MTA Angelus, Biodentin (BD) and white portland cement (WPC) on human dental pulp stem cells (HDPSCs).
Materials and Methods:
HDPSCs were cultured in Dulbecco’s modified eagle’s medium (DMEM) with fetal bovine serum (FBS) and antibiotics at 37°C, 90% humidity in a 5% CO2 incubator. Experimental samples were prepared as disks. The viability of HDPSCs was measured by Mosmann’s tetrazolium toxicity (MTT) assay, and odontogenic potential was assessed using alkaline phosphatase (ALP) activity at 24 hours, 7-, 14-, and 21-day intervals. The mean and standard deviations were statistically analyzed by one-way ANOVA and post hoc Scheffe tests using IBM SPSS Software (IBM statistical package for social sciences) – Version 24.0 with a significance level set as P < 0.05.
Results:
All four groups tested using MTT assay showed no toxicity and possess odontogenic potential in all the experimental durations. Experimental group DWPC presented with the highest mean cell viability and ALP activity at all intervals followed by WPC, MTA, and BD (P < 0.05).
Conclusion:
DWPC presented good bioactivity in terms of cell viability and ALP activity. Thus, DWPC could be a promising endodontic material. However, further research is warranted to explore the clinical applicability. |
| format | Article |
| id | doaj-art-0f46c8c66413417da132313134364098 |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-0f46c8c66413417da1323131343640982025-01-13T10:40:23ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3951S395510.4103/jpbs.jpbs_1078_24Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro StudyG. R. Ishwarya DheviPonnudurai ArangannalJ JeevarathanBackground and Objective: Bioceramic materials have revolutionized the field of endodontics by successfully transforming the outcomes of pulp therapies. Novel biomaterials are evolving by modifying the conventional mineral trioxide aggregate (MTA) to overcome their existing limitations, the major ones being prolonged setting and cytotoxic radiopacifiers. Dental white portland cement (DWPC) is a novel formulated bioceramic material introduced as an enhanced MTA alternative. This in vitro study aims to investigate the cytotoxicity and odontogenic differentiation of novel formulation DWPC with MTA Angelus, Biodentin (BD) and white portland cement (WPC) on human dental pulp stem cells (HDPSCs). Materials and Methods: HDPSCs were cultured in Dulbecco’s modified eagle’s medium (DMEM) with fetal bovine serum (FBS) and antibiotics at 37°C, 90% humidity in a 5% CO2 incubator. Experimental samples were prepared as disks. The viability of HDPSCs was measured by Mosmann’s tetrazolium toxicity (MTT) assay, and odontogenic potential was assessed using alkaline phosphatase (ALP) activity at 24 hours, 7-, 14-, and 21-day intervals. The mean and standard deviations were statistically analyzed by one-way ANOVA and post hoc Scheffe tests using IBM SPSS Software (IBM statistical package for social sciences) – Version 24.0 with a significance level set as P < 0.05. Results: All four groups tested using MTT assay showed no toxicity and possess odontogenic potential in all the experimental durations. Experimental group DWPC presented with the highest mean cell viability and ALP activity at all intervals followed by WPC, MTA, and BD (P < 0.05). Conclusion: DWPC presented good bioactivity in terms of cell viability and ALP activity. Thus, DWPC could be a promising endodontic material. However, further research is warranted to explore the clinical applicability.https://journals.lww.com/10.4103/jpbs.jpbs_1078_24biodentinebioceramicsdwpcmta |
| spellingShingle | G. R. Ishwarya Dhevi Ponnudurai Arangannal J Jeevarathan Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study Journal of Pharmacy and Bioallied Sciences biodentine bioceramics dwpc mta |
| title | Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study |
| title_full | Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study |
| title_fullStr | Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study |
| title_full_unstemmed | Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study |
| title_short | Comparative Analysis of Cytotoxicity and Odontogenic Differentiation of Novel Bioceramic Material on Human Dental Pulp Stem Cells – An In Vitro Study |
| title_sort | comparative analysis of cytotoxicity and odontogenic differentiation of novel bioceramic material on human dental pulp stem cells an in vitro study |
| topic | biodentine bioceramics dwpc mta |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1078_24 |
| work_keys_str_mv | AT grishwaryadhevi comparativeanalysisofcytotoxicityandodontogenicdifferentiationofnovelbioceramicmaterialonhumandentalpulpstemcellsaninvitrostudy AT ponnuduraiarangannal comparativeanalysisofcytotoxicityandodontogenicdifferentiationofnovelbioceramicmaterialonhumandentalpulpstemcellsaninvitrostudy AT jjeevarathan comparativeanalysisofcytotoxicityandodontogenicdifferentiationofnovelbioceramicmaterialonhumandentalpulpstemcellsaninvitrostudy |