What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?
MSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of ly...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/583984 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558290816466944 |
|---|---|
| author | R. N. Bárcia J. M. Santos M. Filipe M. Teixeira J. P. Martins J. Almeida A. Água-Doce S. C. P. Almeida A. Varela S. Pohl K. E. J. Dittmar S. Calado S. I. Simões M. M. Gaspar M. E. M. Cruz W. Lindenmaier L. Graça H. Cruz P. E. Cruz |
| author_facet | R. N. Bárcia J. M. Santos M. Filipe M. Teixeira J. P. Martins J. Almeida A. Água-Doce S. C. P. Almeida A. Varela S. Pohl K. E. J. Dittmar S. Calado S. I. Simões M. M. Gaspar M. E. M. Cruz W. Lindenmaier L. Graça H. Cruz P. E. Cruz |
| author_sort | R. N. Bárcia |
| collection | DOAJ |
| description | MSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of lymphocyte proliferation and induction of regulatory T cells. Results showed that UCX were less immunogenic and showed higher immunosuppression activity than BM-MSCs. Further, UCX did not need prior activation or priming to exert their immunomodulatory effects. This was further corroborated in vivo in a model of acute inflammation. To elucidate the potency differences observed between UCX and BM-MSCs, gene expression related to immune modulation was analysed in both cell types. Several gene expression profile differences were found between UCX and BM-MSCs, namely decreased expression of HLA-DRA, HO-1, IGFBP1, 4 and 6, ILR1, IL6R and PTGES and increased expression of CD200, CD273, CD274, IL1B, IL-8, LIF and TGFB2. The latter were confirmed at the protein expression level. Overall, these results show that UCX seem to be naturally more potent immunosuppressors and less immunogenic than BM-MSCs. We propose that these differences may be due to increased levels of immunomodulatory surface proteins such as CD200, CD273, CD274 and cytokines such as IL1β, IL-8, LIF and TGFβ2. |
| format | Article |
| id | doaj-art-0f4688eb33f44c479347f4d7395ed55c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0f4688eb33f44c479347f4d7395ed55c2025-02-03T01:32:42ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/583984583984What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?R. N. Bárcia0J. M. Santos1M. Filipe2M. Teixeira3J. P. Martins4J. Almeida5A. Água-Doce6S. C. P. Almeida7A. Varela8S. Pohl9K. E. J. Dittmar10S. Calado11S. I. Simões12M. M. Gaspar13M. E. M. Cruz14W. Lindenmaier15L. Graça16H. Cruz17P. E. Cruz18ECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, PortugalInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, PortugalInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, PortugalHelmholtz Centre for Infection Research, Department of Gene Regulation and Differentiation, Inhoffenstaße 7, 38124 Braunschweig, GermanyHelmholtz Centre for Infection Research, Department of Gene Regulation and Differentiation, Inhoffenstaße 7, 38124 Braunschweig, GermanyResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Avenida Professor Gama Pinto, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Avenida Professor Gama Pinto, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Avenida Professor Gama Pinto, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Avenida Professor Gama Pinto, 1649-003 Lisbon, PortugalHelmholtz Centre for Infection Research, Department of Gene Regulation and Differentiation, Inhoffenstaße 7, 38124 Braunschweig, GermanyInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalECBio S.A. - R&D in Biotechnology, Rua Henrique Paiva Couceiro, 27, 2700-451 Amadora, PortugalMSCs derived from the umbilical cord tissue, termed UCX, were investigated for their immunomodulatory properties and compared to bone marrow-derived MSCs (BM-MSCs), the gold-standard in immunotherapy. Immunogenicity and immunosuppression were assessed by mixed lymphocyte reactions, suppression of lymphocyte proliferation and induction of regulatory T cells. Results showed that UCX were less immunogenic and showed higher immunosuppression activity than BM-MSCs. Further, UCX did not need prior activation or priming to exert their immunomodulatory effects. This was further corroborated in vivo in a model of acute inflammation. To elucidate the potency differences observed between UCX and BM-MSCs, gene expression related to immune modulation was analysed in both cell types. Several gene expression profile differences were found between UCX and BM-MSCs, namely decreased expression of HLA-DRA, HO-1, IGFBP1, 4 and 6, ILR1, IL6R and PTGES and increased expression of CD200, CD273, CD274, IL1B, IL-8, LIF and TGFB2. The latter were confirmed at the protein expression level. Overall, these results show that UCX seem to be naturally more potent immunosuppressors and less immunogenic than BM-MSCs. We propose that these differences may be due to increased levels of immunomodulatory surface proteins such as CD200, CD273, CD274 and cytokines such as IL1β, IL-8, LIF and TGFβ2.http://dx.doi.org/10.1155/2015/583984 |
| spellingShingle | R. N. Bárcia J. M. Santos M. Filipe M. Teixeira J. P. Martins J. Almeida A. Água-Doce S. C. P. Almeida A. Varela S. Pohl K. E. J. Dittmar S. Calado S. I. Simões M. M. Gaspar M. E. M. Cruz W. Lindenmaier L. Graça H. Cruz P. E. Cruz What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? Stem Cells International |
| title | What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? |
| title_full | What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? |
| title_fullStr | What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? |
| title_full_unstemmed | What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? |
| title_short | What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells? |
| title_sort | what makes umbilical cord tissue derived mesenchymal stromal cells superior immunomodulators when compared to bone marrow derived mesenchymal stromal cells |
| url | http://dx.doi.org/10.1155/2015/583984 |
| work_keys_str_mv | AT rnbarcia whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT jmsantos whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT mfilipe whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT mteixeira whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT jpmartins whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT jalmeida whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT aaguadoce whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT scpalmeida whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT avarela whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT spohl whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT kejdittmar whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT scalado whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT sisimoes whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT mmgaspar whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT memcruz whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT wlindenmaier whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT lgraca whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT hcruz whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells AT pecruz whatmakesumbilicalcordtissuederivedmesenchymalstromalcellssuperiorimmunomodulatorswhencomparedtobonemarrowderivedmesenchymalstromalcells |