Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide
The increasing health and environmental risks associated with synthetic chemical pesticides necessitate the exploration of safer, sustainable alternatives for plant protection. This study investigates a novel biosynthesized antimicrobial peptide (AMP) from Lactiplantibacillus argentoratensis strain...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494054/full |
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| author | Ishan Tiwari Ali Asger Bhojiya Devendra Jain S. L. Kothari Mohamed A. El-Sheikh Shalini Porwal |
| author_facet | Ishan Tiwari Ali Asger Bhojiya Devendra Jain S. L. Kothari Mohamed A. El-Sheikh Shalini Porwal |
| author_sort | Ishan Tiwari |
| collection | DOAJ |
| description | The increasing health and environmental risks associated with synthetic chemical pesticides necessitate the exploration of safer, sustainable alternatives for plant protection. This study investigates a novel biosynthesized antimicrobial peptide (AMP) from Lactiplantibacillus argentoratensis strain IT, identified as the amino acid chain PRKGSVAKDVLPDPVYNSKLVTRLINHLMIDGKRG, for its efficacy in controlling bacterial wilt (BW) disease in tomato (Solanum lycopersicum) caused by Ralstonia solanacearum. Our research demonstrates that foliar application of this AMP at a concentration of 200 ppm significantly reduces disease incidence by 49.3% and disease severity by 45.8%. Scanning electron microscopy revealed severe morphological disruptions in the bacterial cells upon exposure to the AMP. Additionally, the AMP enhanced host resistance by elevating defense enzyme activities, leading to notable improvements in plant morphology, including a 95.5% increase in plant length, a 20.1% increase in biomass, and a 96.69% increase in root length. This bifunctional AMP provides dual protection by exerting direct antimicrobial activity against the pathogen and eliciting plant defense mechanisms. These findings underscore the potential of this biologically sourced AMP as a natural agent for combating plant diseases and promoting growth in tomato crops. To the best of our knowledge, this is the first study to demonstrate the use of a foliar spray application of a biosynthesized microbial peptide as biocontrol agent against R. solanacearum. This interaction not only highlights its biocontrol efficacy but also its role in promoting the growth of Solanum lycopersicum thereby increasing overall agricultural yield. |
| format | Article |
| id | doaj-art-0f4616d98d9441cfaf14a8d058d308f5 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0f4616d98d9441cfaf14a8d058d308f52025-08-20T02:39:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14940541494054Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptideIshan Tiwari0Ali Asger Bhojiya1Devendra Jain2S. L. Kothari3Mohamed A. El-Sheikh4Shalini Porwal5Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, IndiaU. S. Ostwal P. G. College, Mohanlal Sukhadia University, Chittorgarh, IndiaDepartment of Molecular Biology and Biotechnology, Maharana Pratap University of Agriculture and Technology, Udaipur, IndiaAmity Institute of Biotechnology, Amity University Jaipur, Jaipur, IndiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi ArabiaAmity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, IndiaThe increasing health and environmental risks associated with synthetic chemical pesticides necessitate the exploration of safer, sustainable alternatives for plant protection. This study investigates a novel biosynthesized antimicrobial peptide (AMP) from Lactiplantibacillus argentoratensis strain IT, identified as the amino acid chain PRKGSVAKDVLPDPVYNSKLVTRLINHLMIDGKRG, for its efficacy in controlling bacterial wilt (BW) disease in tomato (Solanum lycopersicum) caused by Ralstonia solanacearum. Our research demonstrates that foliar application of this AMP at a concentration of 200 ppm significantly reduces disease incidence by 49.3% and disease severity by 45.8%. Scanning electron microscopy revealed severe morphological disruptions in the bacterial cells upon exposure to the AMP. Additionally, the AMP enhanced host resistance by elevating defense enzyme activities, leading to notable improvements in plant morphology, including a 95.5% increase in plant length, a 20.1% increase in biomass, and a 96.69% increase in root length. This bifunctional AMP provides dual protection by exerting direct antimicrobial activity against the pathogen and eliciting plant defense mechanisms. These findings underscore the potential of this biologically sourced AMP as a natural agent for combating plant diseases and promoting growth in tomato crops. To the best of our knowledge, this is the first study to demonstrate the use of a foliar spray application of a biosynthesized microbial peptide as biocontrol agent against R. solanacearum. This interaction not only highlights its biocontrol efficacy but also its role in promoting the growth of Solanum lycopersicum thereby increasing overall agricultural yield.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494054/fullpeptidephytopathogentomatoagriculturebacterial wilt |
| spellingShingle | Ishan Tiwari Ali Asger Bhojiya Devendra Jain S. L. Kothari Mohamed A. El-Sheikh Shalini Porwal Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide Frontiers in Microbiology peptide phytopathogen tomato agriculture bacterial wilt |
| title | Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| title_full | Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| title_fullStr | Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| title_full_unstemmed | Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| title_short | Managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| title_sort | managing tomato bacterial wilt through pathogen suppression and host resistance augmentation using microbial peptide |
| topic | peptide phytopathogen tomato agriculture bacterial wilt |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1494054/full |
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