Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i>
Concerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of <...
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2025-03-01
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| author | Suelen S. Gaspar Júnia Alves-Ferreira Patrícia Moniz Talita Silva-Fernandes Adriana I. R. Silvestre Ivone Torrado Gaetano R. Pesce Florbela Carvalheiro Luís C. Duarte Maria C. Fernandes |
| author_facet | Suelen S. Gaspar Júnia Alves-Ferreira Patrícia Moniz Talita Silva-Fernandes Adriana I. R. Silvestre Ivone Torrado Gaetano R. Pesce Florbela Carvalheiro Luís C. Duarte Maria C. Fernandes |
| author_sort | Suelen S. Gaspar |
| collection | DOAJ |
| description | Concerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of <i>Escherichia coli</i> were subjected to various acid concentrations. Samples were taken during fermentation to monitor growth, sugar consumption, biomass yield, and product yield. With increasing AI, the TG1 strain maintained stable growth (0.102 1/h), while xylose consumption decreased, and product formation improved, making it better suited for high-acetic-acid industrial applications. In contrast, the Tuner strain performed better under low-inhibitor conditions but suffered metabolic inhibition at high AI levels, compensating by increasing lactic acid production—an adaptation absent in TG1. However, Tuner showed greater resistance to formic acid stress, sustaining higher growth and ethanol production, whereas TG1 experienced a greater metabolic decline but maintained stable acetic acid output. Both strains experienced inhibition in formic acid metabolism, but TG1 had a higher yield despite its lower overall robustness in formic acid conditions. The use of TG1 for value-added compounds such as ethanol or formic acid may help to avoid the use of chemicals that eliminate acetic acid. Tuner could be used for lactic acid production, especially in hydrolysates with under moderate concentration. |
| format | Article |
| id | doaj-art-0f34b85bcf4440d38a1529d739543cd4 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-0f34b85bcf4440d38a1529d739543cd42025-08-20T03:43:20ZengMDPI AGMicroorganisms2076-26072025-03-0113360510.3390/microorganisms13030605Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i>Suelen S. Gaspar0Júnia Alves-Ferreira1Patrícia Moniz2Talita Silva-Fernandes3Adriana I. R. Silvestre4Ivone Torrado5Gaetano R. Pesce6Florbela Carvalheiro7Luís C. Duarte8Maria C. Fernandes9Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalDepartment of Agronomy, Food, Natural Resources, Animals and Environment—DAFNAE, University of Padua, Agripolis Campus, Viale dell’Università 16, 35020 Legnaro, PD, ItalyLNEG—National Laboratory of Energy and Geology, Bioenergy and Biorefineries Unit, Estrada do Paço do Lumiar, 22, 1649-038 Lisbon, PortugalLNEG—National Laboratory of Energy and Geology, Bioenergy and Biorefineries Unit, Estrada do Paço do Lumiar, 22, 1649-038 Lisbon, PortugalAlentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL)/Polytechnic Institute of Beja (IPBeja), Apartado 6158, 7801-908 Beja, PortugalConcerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of <i>Escherichia coli</i> were subjected to various acid concentrations. Samples were taken during fermentation to monitor growth, sugar consumption, biomass yield, and product yield. With increasing AI, the TG1 strain maintained stable growth (0.102 1/h), while xylose consumption decreased, and product formation improved, making it better suited for high-acetic-acid industrial applications. In contrast, the Tuner strain performed better under low-inhibitor conditions but suffered metabolic inhibition at high AI levels, compensating by increasing lactic acid production—an adaptation absent in TG1. However, Tuner showed greater resistance to formic acid stress, sustaining higher growth and ethanol production, whereas TG1 experienced a greater metabolic decline but maintained stable acetic acid output. Both strains experienced inhibition in formic acid metabolism, but TG1 had a higher yield despite its lower overall robustness in formic acid conditions. The use of TG1 for value-added compounds such as ethanol or formic acid may help to avoid the use of chemicals that eliminate acetic acid. Tuner could be used for lactic acid production, especially in hydrolysates with under moderate concentration.https://www.mdpi.com/2076-2607/13/3/605biomassbioproductsfermentationmicroorganisms |
| spellingShingle | Suelen S. Gaspar Júnia Alves-Ferreira Patrícia Moniz Talita Silva-Fernandes Adriana I. R. Silvestre Ivone Torrado Gaetano R. Pesce Florbela Carvalheiro Luís C. Duarte Maria C. Fernandes Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> Microorganisms biomass bioproducts fermentation microorganisms |
| title | Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> |
| title_full | Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> |
| title_fullStr | Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> |
| title_full_unstemmed | Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> |
| title_short | Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of <i>Escherichia coli</i> |
| title_sort | influence of inhibitors generated in lignocellulosic hydrolysates from group of acids on the growth of strains tg1 and tuner of i escherichia coli i |
| topic | biomass bioproducts fermentation microorganisms |
| url | https://www.mdpi.com/2076-2607/13/3/605 |
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