Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system

Ochrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. ant...

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Main Authors: Andrea Vila, Hugo Pagella, Gonzalo Vera Bello, Alicia Vicente
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2016-04-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/7532
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author Andrea Vila
Hugo Pagella
Gonzalo Vera Bello
Alicia Vicente
author_facet Andrea Vila
Hugo Pagella
Gonzalo Vera Bello
Alicia Vicente
author_sort Andrea Vila
collection DOAJ
description Ochrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. anthropi by the VITEK 2 system is herein described. A 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a Gram-negative coccobacillus identified as O. anthropi by VITEK 2. The patient’s fever persisted along with positive blood cultures despite specific antimicrobial treatment. Due to this adverse outcome, the patient was interrogated again and admitted having domestic swine. Serological tests were positive for acute brucellosis. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of BC strains identified B. suis biovar 1. Timely identification of Brucella is essential for providing proper treatment to the patient and for advising safe handling of laboratory cultures in biological safety cabinets to prevent laboratory-acquired infection. Countries where brucellosis is endemic must be aware of this possibility.
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spelling doaj-art-0f31bfaeee934271bbe2ee38cc1d205f2025-08-20T02:57:08ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-04-01100410.3855/jidc.7532Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 systemAndrea Vila0Hugo Pagella1Gonzalo Vera Bello2Alicia Vicente3Hospital Italiano de Mendoza, Mendoza, ArgentinaHospital Italiano de Mendoza, Mendoza, ArgentinaMinisterio de Salud de Mendoza, ArgentinaHospital Lencinas, Mendoza, ArgentinaOchrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. anthropi by the VITEK 2 system is herein described. A 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a Gram-negative coccobacillus identified as O. anthropi by VITEK 2. The patient’s fever persisted along with positive blood cultures despite specific antimicrobial treatment. Due to this adverse outcome, the patient was interrogated again and admitted having domestic swine. Serological tests were positive for acute brucellosis. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of BC strains identified B. suis biovar 1. Timely identification of Brucella is essential for providing proper treatment to the patient and for advising safe handling of laboratory cultures in biological safety cabinets to prevent laboratory-acquired infection. Countries where brucellosis is endemic must be aware of this possibility. https://jidc.org/index.php/journal/article/view/7532Brucella suisOchrobactrum anthropizoonosis
spellingShingle Andrea Vila
Hugo Pagella
Gonzalo Vera Bello
Alicia Vicente
Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
Journal of Infection in Developing Countries
Brucella suis
Ochrobactrum anthropi
zoonosis
title Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
title_full Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
title_fullStr Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
title_full_unstemmed Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
title_short Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system
title_sort brucella suis bacteremia misidentified as ochrobactrum anthropi by the vitek 2 system
topic Brucella suis
Ochrobactrum anthropi
zoonosis
url https://jidc.org/index.php/journal/article/view/7532
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