Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs
Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs...
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| Format: | Article |
| Language: | zho |
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Journal of Refrigeration Magazines Agency Co., Ltd.
2012-01-01
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| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.03.062 |
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| author | Yang Bo Shen Li Huang Danhua Liu Baolin |
| author_facet | Yang Bo Shen Li Huang Danhua Liu Baolin |
| author_sort | Yang Bo |
| collection | DOAJ |
| description | Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation. |
| format | Article |
| id | doaj-art-0f24df1a337e46fcb6fbd7f02b730607 |
| institution | DOAJ |
| issn | 0253-4339 |
| language | zho |
| publishDate | 2012-01-01 |
| publisher | Journal of Refrigeration Magazines Agency Co., Ltd. |
| record_format | Article |
| series | Zhileng xuebao |
| spelling | doaj-art-0f24df1a337e46fcb6fbd7f02b7306072025-08-20T02:47:17ZzhoJournal of Refrigeration Magazines Agency Co., Ltd.Zhileng xuebao0253-43392012-01-013366519480Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAsYang BoShen LiHuang DanhuaLiu BaolinDifferent types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation.http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.03.062medical cellular biologycryopreservationhepatocytesugar-alcoholdimethyl-sulfoxide(Me2SO) |
| spellingShingle | Yang Bo Shen Li Huang Danhua Liu Baolin Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs Zhileng xuebao medical cellular biology cryopreservation hepatocyte sugar-alcohol dimethyl-sulfoxide(Me2SO) |
| title | Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| title_full | Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| title_fullStr | Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| title_full_unstemmed | Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| title_short | Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs |
| title_sort | cryopreservation of human hepatocyte by sugar alcohol based cpas |
| topic | medical cellular biology cryopreservation hepatocyte sugar-alcohol dimethyl-sulfoxide(Me2SO) |
| url | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.03.062 |
| work_keys_str_mv | AT yangbo cryopreservationofhumanhepatocytebysugaralcoholbasedcpas AT shenli cryopreservationofhumanhepatocytebysugaralcoholbasedcpas AT huangdanhua cryopreservationofhumanhepatocytebysugaralcoholbasedcpas AT liubaolin cryopreservationofhumanhepatocytebysugaralcoholbasedcpas |