Cryopreservation of Human Hepatocyte by Sugar-alcohol based CPAs
Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Journal of Refrigeration Magazines Agency Co., Ltd.
2012-01-01
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| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.03.062 |
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| Summary: | Different types of sugar alcohol is usually used as cryoprotective agents (CPAs) to preserve human hepatocytes. In this study, a new types of sugar alcohol based CPAs with low toxicity was explored by replacing Me2SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me2SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80℃ for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10% Me2SO as CPAs. The results show that 5% Me2SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation. |
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| ISSN: | 0253-4339 |