Tyrosinase Inhibitory Activity of Methanolic Extract and Fractions from Green Seaweed Ulva lactuca
Ulva lactuca, a widely available green seaweed, has demonstrated promising bioactivity as a tyrosinase inhibitor. This study aimed to evaluate the tyrosinase inhibitory activity of U. lactuca extracts and identify the active compounds responsible. The seaweed was extracted using methanol via macerat...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kementerian Kelautan dan Perikanan
2025-05-01
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| Series: | Squalen |
| Subjects: | |
| Online Access: | https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/squalen/article/view/915 |
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| Summary: | Ulva lactuca, a widely available green seaweed, has demonstrated promising
bioactivity as a tyrosinase inhibitor. This study aimed to evaluate the tyrosinase
inhibitory activity of U. lactuca extracts and identify the active compounds
responsible. The seaweed was extracted using methanol via maceration,
followed by partitioning with hexane, chloroform, and water. Total phenolic
and flavonoid contents were determined using UV-VIS spectrophotometry,
and tyrosinase inhibition was assessed in vitro. Among the tested fractions,
the hexane fraction exhibited the highest tyrosinase inhibitory activity with an
IC50 value of 584.34 ± 35.07 µg/mL, classifying it as an active inhibitor. Although
this activity was weaker than kojic acid (IC50 = 11.07 ± 0.86 µg/mL), the hexane
fraction showed relatively high phenolic (11.66 ± 0.53 mg GAE/g) and flavonoid
(9.75 ± 0.30 mg QE/g) contents. Gas Chromatography-Mass Spectrometry
(GC-MS) analysis identified several bioactive compounds, notably 2,4-
dimethoxycinnamic acid, chalcone, and derivatives such as 2,6-
dihydroxybenzoic acid and phloretin. These findings highlight the potential of
U. lactuca, particularly its hexane fraction, as a source of natural tyrosinase
inhibitors. This opens avenues for its application in the pharmaceutical and
cosmetic industries, especially in formulations targeting hyperpigmentation
and skin brightening. |
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| ISSN: | 2089-5690 2406-9272 |