MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1
<b>Objective</b>: To explore the role and mechanism of miR-27b-3p in treating infantile hemangiomas (IHs) with propranolol and to clarify the cause of the poor efficacy of propranolol in IHs. <b>Methods</b>: Human umbilical vein endothelial cells (HUVECs) were used as the res...
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2025-04-01
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| author | Jing Li Yifei Zhu Linyang Xie Sina Ahmadi Chonghao Yao Hao Cui Xuteng Kang Junbo Tu Sijia Na |
| author_facet | Jing Li Yifei Zhu Linyang Xie Sina Ahmadi Chonghao Yao Hao Cui Xuteng Kang Junbo Tu Sijia Na |
| author_sort | Jing Li |
| collection | DOAJ |
| description | <b>Objective</b>: To explore the role and mechanism of miR-27b-3p in treating infantile hemangiomas (IHs) with propranolol and to clarify the cause of the poor efficacy of propranolol in IHs. <b>Methods</b>: Human umbilical vein endothelial cells (HUVECs) were used as the research model and were treated with 0, 15, 30, 45, 60, and 90 μM of propranolol to explore the best concentration. RNA interference technology was used to regulate the expression of miR-27b-3p. CCK-8, TUNEL, and flow cytometry detected cell proliferation and apoptosis levels. Real-time PCR was used to detect the expression of miR-27b-3p and apoptosis pathway-related mRNA, and Western blotting was used to detect the expression of apoptosis-related proteins. The target relationship between miR-27b-3p and Apaf-1 was analyzed using a double Luciferase report. <b>Results</b>: The most significant inhibitory effect on cell activity of propranolol is at a dose of 30 μM. After propranolol treatment, the expression of miR-27b-3p was downregulated, and the expression of the apoptotic factors Apaf-1, PARP, caspase-9, and caspase-3 was upregulated, which was consistent with the results after the deletion of miR-27b-3p. However, after upregulation of miR-27b-3p, the level of and the expression of apoptotic factors was inhibited. “targetscan.org” gene database analysis found that miR-27b-3p matched the 3′-UTR of Apaf-1 mRNA, and luciferase results showed that miR-27b-3p had a targeted relationship with Apaf-1. <b>Conclusions</b>: The miR-27b-3p target inhibits the expression of Apaf-1, reduces the level of endothelial cell apoptosis, and interferes with the therapeutic effect of propranolol. |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Biomedicines |
| spelling | doaj-art-0ef7c3e2b6004c44aefd24ce7cfa682c2025-08-20T01:56:29ZengMDPI AGBiomedicines2227-90592025-04-01135109210.3390/biomedicines13051092MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1Jing Li0Yifei Zhu1Linyang Xie2Sina Ahmadi3Chonghao Yao4Hao Cui5Xuteng Kang6Junbo Tu7Sijia Na8Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, ChinaKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China<b>Objective</b>: To explore the role and mechanism of miR-27b-3p in treating infantile hemangiomas (IHs) with propranolol and to clarify the cause of the poor efficacy of propranolol in IHs. <b>Methods</b>: Human umbilical vein endothelial cells (HUVECs) were used as the research model and were treated with 0, 15, 30, 45, 60, and 90 μM of propranolol to explore the best concentration. RNA interference technology was used to regulate the expression of miR-27b-3p. CCK-8, TUNEL, and flow cytometry detected cell proliferation and apoptosis levels. Real-time PCR was used to detect the expression of miR-27b-3p and apoptosis pathway-related mRNA, and Western blotting was used to detect the expression of apoptosis-related proteins. The target relationship between miR-27b-3p and Apaf-1 was analyzed using a double Luciferase report. <b>Results</b>: The most significant inhibitory effect on cell activity of propranolol is at a dose of 30 μM. After propranolol treatment, the expression of miR-27b-3p was downregulated, and the expression of the apoptotic factors Apaf-1, PARP, caspase-9, and caspase-3 was upregulated, which was consistent with the results after the deletion of miR-27b-3p. However, after upregulation of miR-27b-3p, the level of and the expression of apoptotic factors was inhibited. “targetscan.org” gene database analysis found that miR-27b-3p matched the 3′-UTR of Apaf-1 mRNA, and luciferase results showed that miR-27b-3p had a targeted relationship with Apaf-1. <b>Conclusions</b>: The miR-27b-3p target inhibits the expression of Apaf-1, reduces the level of endothelial cell apoptosis, and interferes with the therapeutic effect of propranolol.https://www.mdpi.com/2227-9059/13/5/1092infantile hemangiomapropranololmiR-27b-3pApaf-1apoptosis |
| spellingShingle | Jing Li Yifei Zhu Linyang Xie Sina Ahmadi Chonghao Yao Hao Cui Xuteng Kang Junbo Tu Sijia Na MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 Biomedicines infantile hemangioma propranolol miR-27b-3p Apaf-1 apoptosis |
| title | MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 |
| title_full | MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 |
| title_fullStr | MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 |
| title_full_unstemmed | MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 |
| title_short | MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1 |
| title_sort | mir 27b 3p reduces the efficacy of propranolol in the treatment of infantile hemangioma by inhibiting the expression of apaf 1 |
| topic | infantile hemangioma propranolol miR-27b-3p Apaf-1 apoptosis |
| url | https://www.mdpi.com/2227-9059/13/5/1092 |
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