Bacteriostatic activity and mechanism of minerals containing rubidium

Abstract. Background. Metals and their ions have been used to reduce bacterial infection risks. Among them, minerals containing rubidium (MCR), natural minerals containing metal ions, show potential as novel and tunable materials. Objective. This study aimed to investigate the antibacterial activity...

Full description

Saved in:
Bibliographic Details
Main Authors: Yucui Lu, Xianmei Long, Yuanhui Mao, Lijing Wang, Xiayun Liao, Lichun Zhao
Format: Article
Language:English
Published: Wolters Kluwer Health/LWW 2025-06-01
Series:Science of Traditional Chinese Medicine
Online Access:http://journals.lww.com/10.1097/st9.0000000000000064
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849415655641579520
author Yucui Lu
Xianmei Long
Yuanhui Mao
Lijing Wang
Xiayun Liao
Lichun Zhao
author_facet Yucui Lu
Xianmei Long
Yuanhui Mao
Lijing Wang
Xiayun Liao
Lichun Zhao
author_sort Yucui Lu
collection DOAJ
description Abstract. Background. Metals and their ions have been used to reduce bacterial infection risks. Among them, minerals containing rubidium (MCR), natural minerals containing metal ions, show potential as novel and tunable materials. Objective. This study aimed to investigate the antibacterial activity and mechanism of MCR. Methods. The inhibitory effect of MCR on bacteria was clarified using the growth curve method, turbidimetric method, and minimum inhibitory concentration method. Physiological and biochemical indices were employed to investigate the inhibitory mechanism of MCR. Results. The results revealed that MCR inhibited Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli with minimum inhibitory concentrations of 11.95, 2.60, and 2.60 mg/mL, respectively. The inhibitory activity of MCR was insignificant against Bacillus subtilis, Salmonella typhimurium, and Helicobacter pylori at 3.25 mg/mL. Mechanistic assessments showed that MCR affected bacterial conductivity, protein and nucleic acid levels, reducing sugar content, respiratory chain dehydrogenase activity, bacterial lipid peroxidation, intracellular adenosine triphosphate, and extracellular alkaline phosphatase. Conclusion. MCR has bacteriostatic activity and the mechanism primarily involves adhesion to bacteria, disrupting the integrity of their cell walls and membranes, and altering their permeability. This disruption leads to the release of intracellular molecules of various sizes, inhibiting cellular respiration and metabolism, and causing oxidative damage. These combined effects impair cellular functions, affecting cell growth and metabolism, or leading to cell death. These findings provide a theoretical reference for the development of MCR as a bacteriostatic agent.
format Article
id doaj-art-0ef5abb53ef54c628cd9f2d7936e7334
institution Kabale University
issn 2836-922X
2836-9211
language English
publishDate 2025-06-01
publisher Wolters Kluwer Health/LWW
record_format Article
series Science of Traditional Chinese Medicine
spelling doaj-art-0ef5abb53ef54c628cd9f2d7936e73342025-08-20T03:33:27ZengWolters Kluwer Health/LWWScience of Traditional Chinese Medicine2836-922X2836-92112025-06-013213714410.1097/st9.0000000000000064202506000-00004Bacteriostatic activity and mechanism of minerals containing rubidiumYucui Lu0Xianmei Long1Yuanhui Mao2Lijing Wang3Xiayun Liao4Lichun Zhao5a College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang, Chinac Institute of Traditional Chinese and Zhuang-Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning, Chinac Institute of Traditional Chinese and Zhuang-Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning, Chinab College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Chinab College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Chinaa College of Pharmaceutical Sciences, Guizhou University of Traditional Chinese Medicine, Guiyang, ChinaAbstract. Background. Metals and their ions have been used to reduce bacterial infection risks. Among them, minerals containing rubidium (MCR), natural minerals containing metal ions, show potential as novel and tunable materials. Objective. This study aimed to investigate the antibacterial activity and mechanism of MCR. Methods. The inhibitory effect of MCR on bacteria was clarified using the growth curve method, turbidimetric method, and minimum inhibitory concentration method. Physiological and biochemical indices were employed to investigate the inhibitory mechanism of MCR. Results. The results revealed that MCR inhibited Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli with minimum inhibitory concentrations of 11.95, 2.60, and 2.60 mg/mL, respectively. The inhibitory activity of MCR was insignificant against Bacillus subtilis, Salmonella typhimurium, and Helicobacter pylori at 3.25 mg/mL. Mechanistic assessments showed that MCR affected bacterial conductivity, protein and nucleic acid levels, reducing sugar content, respiratory chain dehydrogenase activity, bacterial lipid peroxidation, intracellular adenosine triphosphate, and extracellular alkaline phosphatase. Conclusion. MCR has bacteriostatic activity and the mechanism primarily involves adhesion to bacteria, disrupting the integrity of their cell walls and membranes, and altering their permeability. This disruption leads to the release of intracellular molecules of various sizes, inhibiting cellular respiration and metabolism, and causing oxidative damage. These combined effects impair cellular functions, affecting cell growth and metabolism, or leading to cell death. These findings provide a theoretical reference for the development of MCR as a bacteriostatic agent.http://journals.lww.com/10.1097/st9.0000000000000064
spellingShingle Yucui Lu
Xianmei Long
Yuanhui Mao
Lijing Wang
Xiayun Liao
Lichun Zhao
Bacteriostatic activity and mechanism of minerals containing rubidium
Science of Traditional Chinese Medicine
title Bacteriostatic activity and mechanism of minerals containing rubidium
title_full Bacteriostatic activity and mechanism of minerals containing rubidium
title_fullStr Bacteriostatic activity and mechanism of minerals containing rubidium
title_full_unstemmed Bacteriostatic activity and mechanism of minerals containing rubidium
title_short Bacteriostatic activity and mechanism of minerals containing rubidium
title_sort bacteriostatic activity and mechanism of minerals containing rubidium
url http://journals.lww.com/10.1097/st9.0000000000000064
work_keys_str_mv AT yucuilu bacteriostaticactivityandmechanismofmineralscontainingrubidium
AT xianmeilong bacteriostaticactivityandmechanismofmineralscontainingrubidium
AT yuanhuimao bacteriostaticactivityandmechanismofmineralscontainingrubidium
AT lijingwang bacteriostaticactivityandmechanismofmineralscontainingrubidium
AT xiayunliao bacteriostaticactivityandmechanismofmineralscontainingrubidium
AT lichunzhao bacteriostaticactivityandmechanismofmineralscontainingrubidium