Prevalence and Molecular Identification of Giardiasis Isolates from Stray Dogs in Erbil Province, Kurdistan Region, Iraq
Giardia lamblia is the intestinal, flagellated protozoan parasite. It should make a species complex and comprises eight assemblages (A-H). In the current study, out of 153 examined samples, 16 (10.46%) and 36 (23.53%) specimens were positive by concentration and polymerase chain reaction (PCR) techn...
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| Format: | Article |
| Language: | English |
| Published: |
Erbil Polytechnic University
2021-06-01
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| Series: | Polytechnic Journal |
| Subjects: | |
| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol11/iss1/2 |
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| Summary: | Giardia lamblia is the intestinal, flagellated protozoan parasite. It should make a species complex and
comprises eight assemblages (A-H). In the current study, out of 153 examined samples, 16 (10.46%) and
36 (23.53%) specimens were positive by concentration and polymerase chain reaction (PCR) technique
for giardiasis, respectively. The highest rate of infection was found in a rural area (14.38%) than in urban
areas (9.15%). As well as, the infection rate in males (25/153) was higher compared to that of female dogs
(11/153). Regarding fecal consistency, the highest rate (18.3%) of giardiasis was observed in diarrheic
dogs, while the lowest rate of giardiasis (5.23%) was observed among non-diarrheic dogs. The PCR
products were sequenced for 20 samples and further examined by sequence analysis, 16 isolates under the
accession number (MN629930), independent of the host, exhibited G. lamblia GenBank ID: M36728, while
in four samples under the accession number (MN629931), nucleotide substitutions generate polymorphism
at position 542 and 561 (C → T) and (A → G) at position 684. The similarity between MN629931 and
AY072723 genotype A2 was 99.9% which was one nucleotide substitution at position 542 (C → T). The
sequencing of the PCR products recognized two assemblages in dogs suggested the possible role of dogs
as the reservoir for human giardiasis in Erbil Province which is the first records in the Kurdistan Region, Iraq. |
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| ISSN: | 2707-7799 |