3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold
Objective To fabricate 3D biomimetic design of tissue engineering scaffolds（Shell-Core scaffolds）incorporating mesenchymal stem cells（MSCs）and human umbilical vein endothelial cells（HUVECs）by using advanced coaxial 3D bioprinting technology，and to validate the biomimetic vascularization capacity of...
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Editorial Office of Journal of New Medicine
2025-05-01
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| Series: | Xin yixue |
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| Online Access: | https://www.xinyixue.cn/fileup/0253-9802/PDF/1748392624474-936109432.pdf |
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| author | TAN Jiameng, WU Yu, CHEN Jianwei, ZHU Delong, WANG Kun, ZHU Lei |
| author_facet | TAN Jiameng, WU Yu, CHEN Jianwei, ZHU Delong, WANG Kun, ZHU Lei |
| author_sort | TAN Jiameng, WU Yu, CHEN Jianwei, ZHU Delong, WANG Kun, ZHU Lei |
| collection | DOAJ |
| description | Objective To fabricate 3D biomimetic design of tissue engineering scaffolds（Shell-Core scaffolds）incorporating mesenchymal stem cells（MSCs）and human umbilical vein endothelial cells（HUVECs）by using advanced coaxial 3D bioprinting technology，and to validate the biomimetic vascularization capacity of the scaffolds by experiments. Methods The Shell-Core scaffolds were successfully fabricated via coaxial 3D bioprinting technology. The biomimetic structural features were characterized using optical microscopy and histological staining assay. The <i>in vitro</i> pro-angiogenic capacity was evaluated through inter-group comparative observations and cell scratch assays. Furthermore，qRT-PCR was employed to quantify RNA expression levels of angiogenesis-related markers in cells cultured on the Shell-Core scaffolds. Results Shell-Core scaffolds were successfully fabricated. The scaffolds possessed high structural fidelity，which could be maintained at 7 d after scaffold fabrication. Cell proliferation assay showed that the cell proliferation rate in the scaffolds at 7 d was higher than that in the mixed culture on the 2D plane. Cell scratch assay showed that the shortening scratch distance of HUVECs treated by Shell-Core-CM was significantly greater than those treated by 3D-Mix-CM and blank groups [（431.6±33.6）μm <i>vs.</i>（378.7±22.5）μm <i>vs.</i>（302.3±20.1）μm，both <i>P < </i>0.01]. At 7 d after <i>in vitro</i> cultured of engineered biomimetic tissues，under fluorescence microscope，HUVECs expressing green fluorescent protein remained in the designed core channel，and self-assembled endothelial buds in all directions. Furthermore，the results of qRT-PCR show that quantified RNA expression levels of angiogenesis-related markers（MMP-9）in cells cultured on the Shell-Core scaffolds was significantly higher（1.55±0.06，<i>P < </i>0.01）than that in 3D-Mix scaffolds. Conclusions The Shell-Core scaffolds integrating MSCs and HUVECs demonstrates high structural fidelity and pro-angiogenic capacity，offering a novel strategy for addressing tissue defect repair and fabricating vascularized engineered organs. This platform further provides a physiologically relevant tissue model for drug testing and mechanistic investigation of angiogenesis. |
| format | Article |
| id | doaj-art-0edb24bb2f2e4e3bb9b0d9aec7db7c37 |
| institution | DOAJ |
| issn | 0253-9802 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Editorial Office of Journal of New Medicine |
| record_format | Article |
| series | Xin yixue |
| spelling | doaj-art-0edb24bb2f2e4e3bb9b0d9aec7db7c372025-08-20T03:12:12ZzhoEditorial Office of Journal of New MedicineXin yixue0253-98022025-05-0156545846510.12464/j.issn.0253-9802.2025-00783D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffoldTAN Jiameng, WU Yu, CHEN Jianwei, ZHU Delong, WANG Kun, ZHU Lei01. Department of Plastic and Aesthetic Surgery，the Third Affiliated Hospital of Sun Yat-sen University，Guangzhou 510630，China;2. Bio-Intelligent Manufacturing and Bioprinting Research Center，Shenzhen Tsinghua Research Institute，Shenzhen 518063，China;3. Department of Joint and Trauma Surgery，the Third Affiliated Hospital of Sun Yat-sen University，Guangzhou 510630，ChinaObjective To fabricate 3D biomimetic design of tissue engineering scaffolds（Shell-Core scaffolds）incorporating mesenchymal stem cells（MSCs）and human umbilical vein endothelial cells（HUVECs）by using advanced coaxial 3D bioprinting technology，and to validate the biomimetic vascularization capacity of the scaffolds by experiments. Methods The Shell-Core scaffolds were successfully fabricated via coaxial 3D bioprinting technology. The biomimetic structural features were characterized using optical microscopy and histological staining assay. The <i>in vitro</i> pro-angiogenic capacity was evaluated through inter-group comparative observations and cell scratch assays. Furthermore，qRT-PCR was employed to quantify RNA expression levels of angiogenesis-related markers in cells cultured on the Shell-Core scaffolds. Results Shell-Core scaffolds were successfully fabricated. The scaffolds possessed high structural fidelity，which could be maintained at 7 d after scaffold fabrication. Cell proliferation assay showed that the cell proliferation rate in the scaffolds at 7 d was higher than that in the mixed culture on the 2D plane. Cell scratch assay showed that the shortening scratch distance of HUVECs treated by Shell-Core-CM was significantly greater than those treated by 3D-Mix-CM and blank groups [（431.6±33.6）μm <i>vs.</i>（378.7±22.5）μm <i>vs.</i>（302.3±20.1）μm，both <i>P < </i>0.01]. At 7 d after <i>in vitro</i> cultured of engineered biomimetic tissues，under fluorescence microscope，HUVECs expressing green fluorescent protein remained in the designed core channel，and self-assembled endothelial buds in all directions. Furthermore，the results of qRT-PCR show that quantified RNA expression levels of angiogenesis-related markers（MMP-9）in cells cultured on the Shell-Core scaffolds was significantly higher（1.55±0.06，<i>P < </i>0.01）than that in 3D-Mix scaffolds. Conclusions The Shell-Core scaffolds integrating MSCs and HUVECs demonstrates high structural fidelity and pro-angiogenic capacity，offering a novel strategy for addressing tissue defect repair and fabricating vascularized engineered organs. This platform further provides a physiologically relevant tissue model for drug testing and mechanistic investigation of angiogenesis.https://www.xinyixue.cn/fileup/0253-9802/PDF/1748392624474-936109432.pdfcoaxial 3d bioprinting|pre-vascularization|biomimetic design|mesenchymal stem cell|angiogenesis|tissue repair |
| spellingShingle | TAN Jiameng, WU Yu, CHEN Jianwei, ZHU Delong, WANG Kun, ZHU Lei 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold Xin yixue coaxial 3d bioprinting|pre-vascularization|biomimetic design|mesenchymal stem cell|angiogenesis|tissue repair |
| title | 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| title_full | 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| title_fullStr | 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| title_full_unstemmed | 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| title_short | 3D biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| title_sort | 3d biomimetic design of mesenchymal stem cells and endothelial cells promotes the vascularization of tissue engineering scaffold |
| topic | coaxial 3d bioprinting|pre-vascularization|biomimetic design|mesenchymal stem cell|angiogenesis|tissue repair |
| url | https://www.xinyixue.cn/fileup/0253-9802/PDF/1748392624474-936109432.pdf |
| work_keys_str_mv | AT tanjiamengwuyuchenjianweizhudelongwangkunzhulei 3dbiomimeticdesignofmesenchymalstemcellsandendothelialcellspromotesthevascularizationoftissueengineeringscaffold |