Infrared video thermography: a technique for assessing cold adaptation in insects
Insects can survive subzero temperatures by two main strategies: freeze tolerance and freeze avoidance. An array of techniques have been used to investigate the physiological limits of insects to low temperatures, such as differential scanning calorimetry, temperature-controlled cooling apparatus, t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2004-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04372BI01 |
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| _version_ | 1850152193045299200 |
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| author | Christopher M. Palmer Katharina Siebke David K. Yeates |
| author_facet | Christopher M. Palmer Katharina Siebke David K. Yeates |
| author_sort | Christopher M. Palmer |
| collection | DOAJ |
| description | Insects can survive subzero temperatures by two main strategies: freeze tolerance and freeze avoidance. An array of techniques have been used to investigate the physiological limits of insects to low temperatures, such as differential scanning calorimetry, temperature-controlled cooling apparatus, thermocouples, and computer-controlled chart recording equipment. However, these techniques require animals to be stationary, precluding behavioral data. We used infrared video thermography to investigate cold adaptation in an alpine insect, expanding such investigations to include behavioral response as an indicator of physiological stress. This technique is noninvasive and provides a large amount of physiological information, such as supercooling points, lower lethal temperatures, and hemolymph melting points. Insect supercooling points in response to a constant cooling rate were variable; however, temperatures at the initiation of behavioral stress response were less variable. Assessments of supercooling points and lower lethal temperatures obtained in this way are more biologically meaningful because allowing unhindered movement of insects more closely resembles natural environments. |
| format | Article |
| id | doaj-art-0ed40781453f4e6d876f7f0c1bd55cbd |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2004-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-0ed40781453f4e6d876f7f0c1bd55cbd2025-08-20T02:26:03ZengTaylor & Francis GroupBioTechniques0736-62051940-98182004-08-0137221221710.2144/04372BI01Infrared video thermography: a technique for assessing cold adaptation in insectsChristopher M. Palmer0Katharina Siebke1David K. Yeates21The Australian National University1The Australian National University2CSIRO Entomology, Canberra, AustraliaInsects can survive subzero temperatures by two main strategies: freeze tolerance and freeze avoidance. An array of techniques have been used to investigate the physiological limits of insects to low temperatures, such as differential scanning calorimetry, temperature-controlled cooling apparatus, thermocouples, and computer-controlled chart recording equipment. However, these techniques require animals to be stationary, precluding behavioral data. We used infrared video thermography to investigate cold adaptation in an alpine insect, expanding such investigations to include behavioral response as an indicator of physiological stress. This technique is noninvasive and provides a large amount of physiological information, such as supercooling points, lower lethal temperatures, and hemolymph melting points. Insect supercooling points in response to a constant cooling rate were variable; however, temperatures at the initiation of behavioral stress response were less variable. Assessments of supercooling points and lower lethal temperatures obtained in this way are more biologically meaningful because allowing unhindered movement of insects more closely resembles natural environments.https://www.future-science.com/doi/10.2144/04372BI01 |
| spellingShingle | Christopher M. Palmer Katharina Siebke David K. Yeates Infrared video thermography: a technique for assessing cold adaptation in insects BioTechniques |
| title | Infrared video thermography: a technique for assessing cold adaptation in insects |
| title_full | Infrared video thermography: a technique for assessing cold adaptation in insects |
| title_fullStr | Infrared video thermography: a technique for assessing cold adaptation in insects |
| title_full_unstemmed | Infrared video thermography: a technique for assessing cold adaptation in insects |
| title_short | Infrared video thermography: a technique for assessing cold adaptation in insects |
| title_sort | infrared video thermography a technique for assessing cold adaptation in insects |
| url | https://www.future-science.com/doi/10.2144/04372BI01 |
| work_keys_str_mv | AT christophermpalmer infraredvideothermographyatechniqueforassessingcoldadaptationininsects AT katharinasiebke infraredvideothermographyatechniqueforassessingcoldadaptationininsects AT davidkyeates infraredvideothermographyatechniqueforassessingcoldadaptationininsects |