Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging

Mitochondria are of central importance for energy generation in skeletal muscles. Expression changes or functional alterations in mitochondrial enzymes play a key role during myogenesis, fibre maturation, and various neuromuscular pathologies, as well as natural fibre aging. Mass spectrometry-based...

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Main Authors: Lisa Staunton, Kathleen O'Connell, Kay Ohlendieck
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Journal of Aging Research
Online Access:http://dx.doi.org/10.4061/2011/908035
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author Lisa Staunton
Kathleen O'Connell
Kay Ohlendieck
author_facet Lisa Staunton
Kathleen O'Connell
Kay Ohlendieck
author_sort Lisa Staunton
collection DOAJ
description Mitochondria are of central importance for energy generation in skeletal muscles. Expression changes or functional alterations in mitochondrial enzymes play a key role during myogenesis, fibre maturation, and various neuromuscular pathologies, as well as natural fibre aging. Mass spectrometry-based proteomics suggests itself as a convenient large-scale and high-throughput approach to catalogue the mitochondrial protein complement and determine global changes during health and disease. This paper gives a brief overview of the relatively new field of mitochondrial proteomics and discusses the findings from recent proteomic surveys of mitochondrial elements in aged skeletal muscles. Changes in the abundance, biochemical activity, subcellular localization, and/or posttranslational modifications in key mitochondrial enzymes might be useful as novel biomarkers of aging. In the long term, this may advance diagnostic procedures, improve the monitoring of disease progression, help in the testing of side effects due to new drug regimes, and enhance our molecular understanding of age-related muscle degeneration.
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spelling doaj-art-0ecd6bbad0bc41dda9389e10e347af922025-08-20T03:54:24ZengWileyJournal of Aging Research2090-22122011-01-01201110.4061/2011/908035908035Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle AgingLisa Staunton0Kathleen O'Connell1Kay Ohlendieck2Department of Biology, National University of Ireland, Maynooth, County Kildare, IrelandNational Institute for Cellular Biotechnology, Dublin City University, Dublin 9, IrelandDepartment of Biology, National University of Ireland, Maynooth, County Kildare, IrelandMitochondria are of central importance for energy generation in skeletal muscles. Expression changes or functional alterations in mitochondrial enzymes play a key role during myogenesis, fibre maturation, and various neuromuscular pathologies, as well as natural fibre aging. Mass spectrometry-based proteomics suggests itself as a convenient large-scale and high-throughput approach to catalogue the mitochondrial protein complement and determine global changes during health and disease. This paper gives a brief overview of the relatively new field of mitochondrial proteomics and discusses the findings from recent proteomic surveys of mitochondrial elements in aged skeletal muscles. Changes in the abundance, biochemical activity, subcellular localization, and/or posttranslational modifications in key mitochondrial enzymes might be useful as novel biomarkers of aging. In the long term, this may advance diagnostic procedures, improve the monitoring of disease progression, help in the testing of side effects due to new drug regimes, and enhance our molecular understanding of age-related muscle degeneration.http://dx.doi.org/10.4061/2011/908035
spellingShingle Lisa Staunton
Kathleen O'Connell
Kay Ohlendieck
Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
Journal of Aging Research
title Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
title_full Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
title_fullStr Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
title_full_unstemmed Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
title_short Proteomic Profiling of Mitochondrial Enzymes during Skeletal Muscle Aging
title_sort proteomic profiling of mitochondrial enzymes during skeletal muscle aging
url http://dx.doi.org/10.4061/2011/908035
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AT kathleenoconnell proteomicprofilingofmitochondrialenzymesduringskeletalmuscleaging
AT kayohlendieck proteomicprofilingofmitochondrialenzymesduringskeletalmuscleaging