Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis

Abstract Background Liver injury is associated with cholelithiasis, with changes in liver enzyme levels potentially influencing cholelithiasis risk. This study investigates the shared genetic basis between serum levels of four liver enzymes and cholelithiasis using summary data from large-scale geno...

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Main Authors: Wenhao Tian, Zixin Wu, Wen Yang, Hongyang Wang, Qiyu Feng
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Gastroenterology
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Online Access:https://doi.org/10.1186/s12876-025-04162-w
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author Wenhao Tian
Zixin Wu
Wen Yang
Hongyang Wang
Qiyu Feng
author_facet Wenhao Tian
Zixin Wu
Wen Yang
Hongyang Wang
Qiyu Feng
author_sort Wenhao Tian
collection DOAJ
description Abstract Background Liver injury is associated with cholelithiasis, with changes in liver enzyme levels potentially influencing cholelithiasis risk. This study investigates the shared genetic basis between serum levels of four liver enzymes and cholelithiasis using summary data from large-scale genome-wide association studies (GWAS). Methods We assessed genetic correlation between liver enzymes and cholelithiasis, and performed local genetic correlation analysis to identify shared genomic regions. A cross-trait meta-analysis identified significant SNPs (single nucleotide polymorphisms) shared between the enzymes and cholelithiasis. To explore causal effects, we applied both two-sample Mendelian randomization and multivariable Mendelian randomization. Heritability-based enrichment analysis was employed to identify tissues and cells jointly associated with liver enzymes and cholelithiasis, while summary-data-based Mendelian Randomization (SMR) was utilized to identify shared genes. Results Alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) showed relatively stronger genetic correlations with cholelithiasis compared to the other liver enzymes. Shared SNPs were identified among ALT, GGT, alkaline phosphatase (ALP), and cholelithiasis. Mendelian randomization indicated that a tenfold increase in ALT could raise cholelithiasis risk by 203.4%. The liver was identified as the primary tissue linking these enzymes to cholelithiasis, but no shared cell types were implicated. Several candidate genes, such as SPTLC3, may jointly influence liver enzyme levels and cholelithiasis risk. Conclusions Elevated ALT levels may increase cholelithiasis risk. Genetic associations across tissues, genes, and SNPs suggest that liver enzymes could mediate the relationship between liver injury and cholelithiasis risk, providing insights into shared genetic mechanisms with potential implications for future research.
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spelling doaj-art-0ec8ac8edc164ef18c9b63ca447e6d242025-08-20T04:03:07ZengBMCBMC Gastroenterology1471-230X2025-08-0125111810.1186/s12876-025-04162-wInvestigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasisWenhao Tian0Zixin Wu1Wen Yang2Hongyang Wang3Qiyu Feng4Cancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of ChinaAbstract Background Liver injury is associated with cholelithiasis, with changes in liver enzyme levels potentially influencing cholelithiasis risk. This study investigates the shared genetic basis between serum levels of four liver enzymes and cholelithiasis using summary data from large-scale genome-wide association studies (GWAS). Methods We assessed genetic correlation between liver enzymes and cholelithiasis, and performed local genetic correlation analysis to identify shared genomic regions. A cross-trait meta-analysis identified significant SNPs (single nucleotide polymorphisms) shared between the enzymes and cholelithiasis. To explore causal effects, we applied both two-sample Mendelian randomization and multivariable Mendelian randomization. Heritability-based enrichment analysis was employed to identify tissues and cells jointly associated with liver enzymes and cholelithiasis, while summary-data-based Mendelian Randomization (SMR) was utilized to identify shared genes. Results Alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) showed relatively stronger genetic correlations with cholelithiasis compared to the other liver enzymes. Shared SNPs were identified among ALT, GGT, alkaline phosphatase (ALP), and cholelithiasis. Mendelian randomization indicated that a tenfold increase in ALT could raise cholelithiasis risk by 203.4%. The liver was identified as the primary tissue linking these enzymes to cholelithiasis, but no shared cell types were implicated. Several candidate genes, such as SPTLC3, may jointly influence liver enzyme levels and cholelithiasis risk. Conclusions Elevated ALT levels may increase cholelithiasis risk. Genetic associations across tissues, genes, and SNPs suggest that liver enzymes could mediate the relationship between liver injury and cholelithiasis risk, providing insights into shared genetic mechanisms with potential implications for future research.https://doi.org/10.1186/s12876-025-04162-wCholelithiasisLiver injuryLiver enzymesGWASShared genetic information
spellingShingle Wenhao Tian
Zixin Wu
Wen Yang
Hongyang Wang
Qiyu Feng
Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
BMC Gastroenterology
Cholelithiasis
Liver injury
Liver enzymes
GWAS
Shared genetic information
title Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
title_full Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
title_fullStr Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
title_full_unstemmed Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
title_short Investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
title_sort investigating the shared genetic information between serum concentration levels of liver enzymes and cholelithiasis
topic Cholelithiasis
Liver injury
Liver enzymes
GWAS
Shared genetic information
url https://doi.org/10.1186/s12876-025-04162-w
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