Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns
Abstract The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell gro...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-79307-0 |
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| author | Frédéric Leprêtre Jean-Pascal Meneboo Céline Villenet Laure Delestré Bruno Quesnel Carl Simon Shelley Martin Figeac Sylvie Galiègue-Zouitina |
| author_facet | Frédéric Leprêtre Jean-Pascal Meneboo Céline Villenet Laure Delestré Bruno Quesnel Carl Simon Shelley Martin Figeac Sylvie Galiègue-Zouitina |
| author_sort | Frédéric Leprêtre |
| collection | DOAJ |
| description | Abstract The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell growth and survival. We previously analyzed the RHOH gene structure and demonstrated that this gene is composed of 7 exons, one single encoding exon located at the 3ʹ extremity of the gene, preceded by 6 noncoding exons. To further understand the transcription events associated with this gene, we performed full-length RNA-Seq on 12 B-cell lines. We identified new exons, new splice events and new splice sites, leading to the discovery of 38 RHOH mRNA molecules, 27 of which have never been described before. Here, we also describe new fusion transcripts. Moreover, our method allowed quantitative measurements of the different mRNA species relative to each other in relation to B-cell differentiation. |
| format | Article |
| id | doaj-art-0ec6757a24ae40658dd75c324332f55e |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-0ec6757a24ae40658dd75c324332f55e2025-08-20T02:10:46ZengNature PortfolioScientific Reports2045-23222024-11-0114111510.1038/s41598-024-79307-0Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patternsFrédéric Leprêtre0Jean-Pascal Meneboo1Céline Villenet2Laure Delestré3Bruno Quesnel4Carl Simon Shelley5Martin Figeac6Sylvie Galiègue-Zouitina7Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UAR 2014-PLBSUniv. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UAR 2014-PLBSUniv. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UAR 2014-PLBSInserm U1170CHU Lille, UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, Université de LilleLeukemia Therapeutics, LLCUniv. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UAR 2014-PLBSInstitut pour la Recherche sur le Cancer de LilleAbstract The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell growth and survival. We previously analyzed the RHOH gene structure and demonstrated that this gene is composed of 7 exons, one single encoding exon located at the 3ʹ extremity of the gene, preceded by 6 noncoding exons. To further understand the transcription events associated with this gene, we performed full-length RNA-Seq on 12 B-cell lines. We identified new exons, new splice events and new splice sites, leading to the discovery of 38 RHOH mRNA molecules, 27 of which have never been described before. Here, we also describe new fusion transcripts. Moreover, our method allowed quantitative measurements of the different mRNA species relative to each other in relation to B-cell differentiation.https://doi.org/10.1038/s41598-024-79307-0RHOH geneFull-length targeted mRNA-SeqOxford Nanopore TechnologiesNew exonsNew transcriptsGene fusion |
| spellingShingle | Frédéric Leprêtre Jean-Pascal Meneboo Céline Villenet Laure Delestré Bruno Quesnel Carl Simon Shelley Martin Figeac Sylvie Galiègue-Zouitina Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns Scientific Reports RHOH gene Full-length targeted mRNA-Seq Oxford Nanopore Technologies New exons New transcripts Gene fusion |
| title | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
| title_full | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
| title_fullStr | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
| title_full_unstemmed | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
| title_short | Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns |
| title_sort | full length rna seq of the rhoh gene in human b cells reveals new exons and splicing patterns |
| topic | RHOH gene Full-length targeted mRNA-Seq Oxford Nanopore Technologies New exons New transcripts Gene fusion |
| url | https://doi.org/10.1038/s41598-024-79307-0 |
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