Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand.
Indole-3-acetic acid (IAA) is an imperative phytohormone for plant growth and development. Ectomycorrhizal fungi (ECM) are able to produce IAA. However, only a few studies on IAA biosynthesis pathways in ECM fungi have been reported. This study aimed to investigate the IAA biosynthesis pathway of si...
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227478&type=printable |
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| author | Jaturong Kumla Nakarin Suwannarach Kenji Matsui Saisamorn Lumyong |
| author_facet | Jaturong Kumla Nakarin Suwannarach Kenji Matsui Saisamorn Lumyong |
| author_sort | Jaturong Kumla |
| collection | DOAJ |
| description | Indole-3-acetic acid (IAA) is an imperative phytohormone for plant growth and development. Ectomycorrhizal fungi (ECM) are able to produce IAA. However, only a few studies on IAA biosynthesis pathways in ECM fungi have been reported. This study aimed to investigate the IAA biosynthesis pathway of six ECM cultures including Astraeus odoratus, Gyrodon suthepensis, Phlebopus portentosus, Pisolithus albus, Pisolithus orientalis and Scleroderma suthepense. The results showed that all ECM fungi produced IAA in liquid medium that had been supplemented with L-tryptophan. Notably, fungal IAA levels vary for different fungal species. The detection of indole-3-lactic acid and indole-3-ethanol in the crude culture extracts of all ECM fungi indicated an enzymatic reduction of indole-3-pyruvic acid and indole-3-acetaldehyde, respectively in the IAA biosynthesis via the indole-3-pyruvic acid pathway. Moreover, the tryptophan aminotransferase activity confirmed that all ECM fungi synthesize IAA through the indole-3-pyruvic acid pathway. Additionally, the elongation of rice and oat coleoptiles was stimulated by crude culture extract. This is the first report of the biosynthesis pathway of IAA in the tested ECM fungi. |
| format | Article |
| id | doaj-art-0ebd3bbf0f3d4aca8b06a561ebb7ede7 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-0ebd3bbf0f3d4aca8b06a561ebb7ede72025-08-20T02:55:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022747810.1371/journal.pone.0227478Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand.Jaturong KumlaNakarin SuwannarachKenji MatsuiSaisamorn LumyongIndole-3-acetic acid (IAA) is an imperative phytohormone for plant growth and development. Ectomycorrhizal fungi (ECM) are able to produce IAA. However, only a few studies on IAA biosynthesis pathways in ECM fungi have been reported. This study aimed to investigate the IAA biosynthesis pathway of six ECM cultures including Astraeus odoratus, Gyrodon suthepensis, Phlebopus portentosus, Pisolithus albus, Pisolithus orientalis and Scleroderma suthepense. The results showed that all ECM fungi produced IAA in liquid medium that had been supplemented with L-tryptophan. Notably, fungal IAA levels vary for different fungal species. The detection of indole-3-lactic acid and indole-3-ethanol in the crude culture extracts of all ECM fungi indicated an enzymatic reduction of indole-3-pyruvic acid and indole-3-acetaldehyde, respectively in the IAA biosynthesis via the indole-3-pyruvic acid pathway. Moreover, the tryptophan aminotransferase activity confirmed that all ECM fungi synthesize IAA through the indole-3-pyruvic acid pathway. Additionally, the elongation of rice and oat coleoptiles was stimulated by crude culture extract. This is the first report of the biosynthesis pathway of IAA in the tested ECM fungi.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227478&type=printable |
| spellingShingle | Jaturong Kumla Nakarin Suwannarach Kenji Matsui Saisamorn Lumyong Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. PLoS ONE |
| title | Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. |
| title_full | Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. |
| title_fullStr | Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. |
| title_full_unstemmed | Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. |
| title_short | Biosynthetic pathway of indole-3-acetic acid in ectomycorrhizal fungi collected from northern Thailand. |
| title_sort | biosynthetic pathway of indole 3 acetic acid in ectomycorrhizal fungi collected from northern thailand |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227478&type=printable |
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