Multimodal real-time imaging with laser speckle contrast and fluorescent contrast
Introduction: Laser speckle contrast imaging (LSCI) can achieve real-time 2D perfusion maps non-invasively. However, LSCI is still difficult to use in general clinical applications because of movement sensitivity and limitations in blood flow analysis. To overcome this, fluorescence imaging (FI) is...
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Elsevier
2024-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100023006397 |
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| author | Hyun-Seo Park Min-Jae Shim Yikeun Kim Taek-Yong Ko Jin-Hyuk Choi Yeh-Chan Ahn |
| author_facet | Hyun-Seo Park Min-Jae Shim Yikeun Kim Taek-Yong Ko Jin-Hyuk Choi Yeh-Chan Ahn |
| author_sort | Hyun-Seo Park |
| collection | DOAJ |
| description | Introduction: Laser speckle contrast imaging (LSCI) can achieve real-time 2D perfusion maps non-invasively. However, LSCI is still difficult to use in general clinical applications because of movement sensitivity and limitations in blood flow analysis. To overcome this, fluorescence imaging (FI) is combined with LSCI using a light source with a wavelength of 785 nm in near-infrared (NIR) region and validates to visualize real-time blood perfusion. Materials and methods: The system was performed using Intralipid and indocyanine green (ICG) in a flow phantom that has three tubes and controlled the flow rate in 0–150 μl/min range. First, real-time LSCI was monitored and measured the change in speckle contrast by reperfusion. Then, we visualized blood perfusion of a rabbit ear under the non-invasive condition by intravenous injection using a total of five different ICG concentration solutions from 128 μM to 3.22 mM. Results: The combined system achieved the performance of processing laser speckle images at about 37–38 fps, and we simultaneously confirmed the fluorescence of ICG and changes in speckle contrast due to intralipid as a light scatterer. In addition, we obtained real-time contrast variation and fluorescent images occurring in rabbit's blood perfusion. Conclusions: The aim of this study is to provide a real-time diagnostic imaging system that can be used in general clinical applications. LSCI and FI are combined complementary for observing tissue perfusion using a single NIR light source. The combined system could achieve real-time visualization of blood perfusion non-invasively. |
| format | Article |
| id | doaj-art-0eb4a2f2b3c84731a2e1ce09299f60ce |
| institution | OA Journals |
| issn | 1572-1000 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
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| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-0eb4a2f2b3c84731a2e1ce09299f60ce2025-08-20T02:39:07ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002024-02-014510391210.1016/j.pdpdt.2023.103912Multimodal real-time imaging with laser speckle contrast and fluorescent contrastHyun-Seo Park0Min-Jae Shim1Yikeun Kim2Taek-Yong Ko3Jin-Hyuk Choi4Yeh-Chan Ahn5Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, South KoreaDepartment of Biomedical Engineering, Pukyong National University, Busan 48513, South KoreaDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, South KoreaKosin University Gospel Hospital, Busan 49267, South KoreaKosin University Gospel Hospital, Busan 49267, South KoreaIndustry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, South Korea; Department of Biomedical Engineering, Pukyong National University, Busan 48513, South Korea; Corresponding author: Department of Biomedical Engineering, Pukyong National University, Busan 48513, South KoreaIntroduction: Laser speckle contrast imaging (LSCI) can achieve real-time 2D perfusion maps non-invasively. However, LSCI is still difficult to use in general clinical applications because of movement sensitivity and limitations in blood flow analysis. To overcome this, fluorescence imaging (FI) is combined with LSCI using a light source with a wavelength of 785 nm in near-infrared (NIR) region and validates to visualize real-time blood perfusion. Materials and methods: The system was performed using Intralipid and indocyanine green (ICG) in a flow phantom that has three tubes and controlled the flow rate in 0–150 μl/min range. First, real-time LSCI was monitored and measured the change in speckle contrast by reperfusion. Then, we visualized blood perfusion of a rabbit ear under the non-invasive condition by intravenous injection using a total of five different ICG concentration solutions from 128 μM to 3.22 mM. Results: The combined system achieved the performance of processing laser speckle images at about 37–38 fps, and we simultaneously confirmed the fluorescence of ICG and changes in speckle contrast due to intralipid as a light scatterer. In addition, we obtained real-time contrast variation and fluorescent images occurring in rabbit's blood perfusion. Conclusions: The aim of this study is to provide a real-time diagnostic imaging system that can be used in general clinical applications. LSCI and FI are combined complementary for observing tissue perfusion using a single NIR light source. The combined system could achieve real-time visualization of blood perfusion non-invasively.http://www.sciencedirect.com/science/article/pii/S1572100023006397Laser speckle contrast imaging (LSCI)Fluorescence imaging (FI)Near-infrared (NIR)Indocyanine green (ICG)Image processingMulti-modal imaging |
| spellingShingle | Hyun-Seo Park Min-Jae Shim Yikeun Kim Taek-Yong Ko Jin-Hyuk Choi Yeh-Chan Ahn Multimodal real-time imaging with laser speckle contrast and fluorescent contrast Photodiagnosis and Photodynamic Therapy Laser speckle contrast imaging (LSCI) Fluorescence imaging (FI) Near-infrared (NIR) Indocyanine green (ICG) Image processing Multi-modal imaging |
| title | Multimodal real-time imaging with laser speckle contrast and fluorescent contrast |
| title_full | Multimodal real-time imaging with laser speckle contrast and fluorescent contrast |
| title_fullStr | Multimodal real-time imaging with laser speckle contrast and fluorescent contrast |
| title_full_unstemmed | Multimodal real-time imaging with laser speckle contrast and fluorescent contrast |
| title_short | Multimodal real-time imaging with laser speckle contrast and fluorescent contrast |
| title_sort | multimodal real time imaging with laser speckle contrast and fluorescent contrast |
| topic | Laser speckle contrast imaging (LSCI) Fluorescence imaging (FI) Near-infrared (NIR) Indocyanine green (ICG) Image processing Multi-modal imaging |
| url | http://www.sciencedirect.com/science/article/pii/S1572100023006397 |
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