ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.

The main goal of Molecular Dynamics (MD) is a simulation of a physical system motions in a fixed time period. This technique allows users to observe the dynamic evolution of the system but requires advanced force fields and is computationally intensive. Furthermore, finding desirable features in the...

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Main Authors: Krzysztof Mularski, Dawid Makalowski, Marcin Okonek, Daria Glebowska, Mateusz Swiercz, Karol Kamel, Jacek Blazewicz, Maciej Antczak, Aleksandra Swiercz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0329314
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author Krzysztof Mularski
Dawid Makalowski
Marcin Okonek
Daria Glebowska
Mateusz Swiercz
Karol Kamel
Jacek Blazewicz
Maciej Antczak
Aleksandra Swiercz
author_facet Krzysztof Mularski
Dawid Makalowski
Marcin Okonek
Daria Glebowska
Mateusz Swiercz
Karol Kamel
Jacek Blazewicz
Maciej Antczak
Aleksandra Swiercz
author_sort Krzysztof Mularski
collection DOAJ
description The main goal of Molecular Dynamics (MD) is a simulation of a physical system motions in a fixed time period. This technique allows users to observe the dynamic evolution of the system but requires advanced force fields and is computationally intensive. Furthermore, finding desirable features in the results obtained is usually a time-consuming task. This motivates the need to implement a tool that visualizes the most flexible protein fragments. ProProtein platform is a sophisticated web server where, with a single click, the user can set up, configure, and run an MD simulation of the 3D structure of the peptide/protein. We perform the simulation using an open-source software suite developed for high-performance molecular dynamics named Gromacs. The resulting MD trajectory is then automatically analyzed within the dedicated heuristic algorithm to identify 3D fragments characterized by high instability in the context of the given (input) structure. These high-fluctuation substructures are presented next to the user with the Mol* package. They are visualized in colors on each frame covered by the considered trajectory. This tool can easily support the evaluation of the reliability of protein 3D structure predictions obtained computationally. The ProProtein platform is free and open to all users. It is publicly available at: https://proprotein.cs.put.poznan.pl/.
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institution Kabale University
issn 1932-6203
language English
publishDate 2025-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-0ea22cc46ba04b16971cb95a4c6861d82025-08-23T05:32:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01208e032931410.1371/journal.pone.0329314ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.Krzysztof MularskiDawid MakalowskiMarcin OkonekDaria GlebowskaMateusz SwierczKarol KamelJacek BlazewiczMaciej AntczakAleksandra SwierczThe main goal of Molecular Dynamics (MD) is a simulation of a physical system motions in a fixed time period. This technique allows users to observe the dynamic evolution of the system but requires advanced force fields and is computationally intensive. Furthermore, finding desirable features in the results obtained is usually a time-consuming task. This motivates the need to implement a tool that visualizes the most flexible protein fragments. ProProtein platform is a sophisticated web server where, with a single click, the user can set up, configure, and run an MD simulation of the 3D structure of the peptide/protein. We perform the simulation using an open-source software suite developed for high-performance molecular dynamics named Gromacs. The resulting MD trajectory is then automatically analyzed within the dedicated heuristic algorithm to identify 3D fragments characterized by high instability in the context of the given (input) structure. These high-fluctuation substructures are presented next to the user with the Mol* package. They are visualized in colors on each frame covered by the considered trajectory. This tool can easily support the evaluation of the reliability of protein 3D structure predictions obtained computationally. The ProProtein platform is free and open to all users. It is publicly available at: https://proprotein.cs.put.poznan.pl/.https://doi.org/10.1371/journal.pone.0329314
spellingShingle Krzysztof Mularski
Dawid Makalowski
Marcin Okonek
Daria Glebowska
Mateusz Swiercz
Karol Kamel
Jacek Blazewicz
Maciej Antczak
Aleksandra Swiercz
ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
PLoS ONE
title ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
title_full ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
title_fullStr ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
title_full_unstemmed ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
title_short ProProtein: A platform for fully automated identification of 3D structure fluctuations in MD simulation trajectories.
title_sort proprotein a platform for fully automated identification of 3d structure fluctuations in md simulation trajectories
url https://doi.org/10.1371/journal.pone.0329314
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