Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease
In rice, leucine-rich repeat nucleotide-binding site (NLR) proteins are pivotal immune receptors in combating <i>Magnaporthe oryzae</i>-triggered rice blast. However, the precise molecular mechanism underlying how NLR proteins regulate downstream signalling remains elusive due to the lac...
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2025-01-01
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| author | Dewei Yang Niqing He Fenghuang Huang Jialin Chen Minxiang Yu Yidan Jin Shaojun Lin Shengping Li |
| author_facet | Dewei Yang Niqing He Fenghuang Huang Jialin Chen Minxiang Yu Yidan Jin Shaojun Lin Shengping Li |
| author_sort | Dewei Yang |
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| description | In rice, leucine-rich repeat nucleotide-binding site (NLR) proteins are pivotal immune receptors in combating <i>Magnaporthe oryzae</i>-triggered rice blast. However, the precise molecular mechanism underlying how NLR proteins regulate downstream signalling remains elusive due to the lack of knowledge regarding their direct downstream targets. The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. At the same time, after knocking out <i>OsRac1</i> in the background of Shuangkang 77009 containing <i>Pigm-1</i>, two knockout lines showed susceptibility to rice blast. This study reveals OsRac1, a GTPase, as a signalling molecule involved in Pigm-1-mediated blast resistance, suggesting its potential as a common downstream effector of rice NLR proteins. Additionally, a transcriptional activator, RAI1, acts as an essential Pigm-1 interactor for blast resistance. Furthermore, a novel material 9311(<i>Pigm-1</i>) was prepared by using two-line restorer line 9311 as receptor and Shuangkang 77009 as donor with molecular marker-assisted technology, which improved blast resistance and yield. This research demonstrates that molecular marker-assisted selection technology enhances both resistance and yield in the crucial two-line restorer 9311(<i>Pigm-1</i>). This study offers crucial insights into how Pigm-1 protein activates downstream molecules and serves as a valuable reference for the molecular breeding of rice blast resistance genes, particularly <i>Pigm-1</i>. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Plants |
| spelling | doaj-art-0ea20b18236742258f3bee7205fbf4432025-01-24T13:46:48ZengMDPI AGPlants2223-77472025-01-0114221710.3390/plants14020217Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast DiseaseDewei Yang0Niqing He1Fenghuang Huang2Jialin Chen3Minxiang Yu4Yidan Jin5Shaojun Lin6Shengping Li7Institute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, ChinaInstitute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, ChinaInstitute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, ChinaCollege of Agriculture and Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, ChinaCollege of Agriculture and Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Rice, Fujian Academy of Agricultural Sciences, Fuzhou 350018, ChinaCollege of Agriculture and Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaIn rice, leucine-rich repeat nucleotide-binding site (NLR) proteins are pivotal immune receptors in combating <i>Magnaporthe oryzae</i>-triggered rice blast. However, the precise molecular mechanism underlying how NLR proteins regulate downstream signalling remains elusive due to the lack of knowledge regarding their direct downstream targets. The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. At the same time, after knocking out <i>OsRac1</i> in the background of Shuangkang 77009 containing <i>Pigm-1</i>, two knockout lines showed susceptibility to rice blast. This study reveals OsRac1, a GTPase, as a signalling molecule involved in Pigm-1-mediated blast resistance, suggesting its potential as a common downstream effector of rice NLR proteins. Additionally, a transcriptional activator, RAI1, acts as an essential Pigm-1 interactor for blast resistance. Furthermore, a novel material 9311(<i>Pigm-1</i>) was prepared by using two-line restorer line 9311 as receptor and Shuangkang 77009 as donor with molecular marker-assisted technology, which improved blast resistance and yield. This research demonstrates that molecular marker-assisted selection technology enhances both resistance and yield in the crucial two-line restorer 9311(<i>Pigm-1</i>). This study offers crucial insights into how Pigm-1 protein activates downstream molecules and serves as a valuable reference for the molecular breeding of rice blast resistance genes, particularly <i>Pigm-1</i>.https://www.mdpi.com/2223-7747/14/2/217Pigm-1OsRac1rice blast resistancesignal transductionmolecular breeding |
| spellingShingle | Dewei Yang Niqing He Fenghuang Huang Jialin Chen Minxiang Yu Yidan Jin Shaojun Lin Shengping Li Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease Plants Pigm-1 OsRac1 rice blast resistance signal transduction molecular breeding |
| title | Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease |
| title_full | Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease |
| title_fullStr | Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease |
| title_full_unstemmed | Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease |
| title_short | Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease |
| title_sort | importance of osrac1 in signalling of pigm 1 mediated resistance to rice blast disease |
| topic | Pigm-1 OsRac1 rice blast resistance signal transduction molecular breeding |
| url | https://www.mdpi.com/2223-7747/14/2/217 |
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