Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model

The critical nitrogen dilution curve (CNDC) model enables precise nitrogen management by quantifying the threshold of nitrogen deficiency in crops, thereby enhancing both crop productivity and nitrogen use efficiency. However, its applicability to perennial crops remains unclear. In this study, alfa...

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Main Authors: Yaya Duan, Yi Ling, Haiyan Li, Wenjing Chang, Jiandong Lu, Minhua Yin, Yanxia Kang, Yanlin Ma, Yayu Wang, Guangping Qi, Guoyun Shen
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/12/1782
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author Yaya Duan
Yi Ling
Haiyan Li
Wenjing Chang
Jiandong Lu
Minhua Yin
Yanxia Kang
Yanlin Ma
Yayu Wang
Guangping Qi
Guoyun Shen
author_facet Yaya Duan
Yi Ling
Haiyan Li
Wenjing Chang
Jiandong Lu
Minhua Yin
Yanxia Kang
Yanlin Ma
Yayu Wang
Guangping Qi
Guoyun Shen
author_sort Yaya Duan
collection DOAJ
description The critical nitrogen dilution curve (CNDC) model enables precise nitrogen management by quantifying the threshold of nitrogen deficiency in crops, thereby enhancing both crop productivity and nitrogen use efficiency. However, its applicability to perennial crops remains unclear. In this study, alfalfa (<i>Medicago sativa</i> L.), a perennial leguminous forage, was used as the model crop. Based on two years of field experiments, CNDC models of aboveground biomass were constructed under two nitrogen fertilizer regimes: urea (0, 80, 160, and 240 kg·ha<sup>−1</sup>, applied in a 6:2:2 basal-to-topdressing ratio) and controlled-release urea (CRU; 0, 80, 160, and 240 kg·ha<sup>−1</sup>, applied as a single basal dose). Using these models, the nitrogen nutrition index (NNI) and cumulative nitrogen deficit (N<sub>and</sub>) models were developed to diagnose alfalfa nitrogen status, and the optimal nitrogen application rates were determined via regression analysis. The results showed that critical nitrogen concentration and aboveground biomass followed a power function relationship under both fertilizer types. For CRU treatments, parameters a and b were 3.41 and 0.20 (first cut), 3.15 and 0.12 (second cut), and 2.24 and 0.40 (third cut), respectively. For urea treatments, a and b were 3.13 and 0.35 (first cut), 2.21 and 0.16 (second cut), and 1.75 and 0.73 (third cut). The normalized root mean square error (n-RMSE) of the models ranged from 3.1% to 13%, indicating high model reliability. Based on the NNI, N<sub>and</sub>, and yield response models, the optimal nitrogen application rates were 175.44~181.71 kg·ha<sup>−1</sup> for urea and 145.63~153.46 kg·ha<sup>−1</sup> for CRU, corresponding to theoretical maximum yields of 14.76~17.40 t·ha<sup>−1</sup> and 16.76~20.66 t·ha<sup>−1</sup>, respectively. Compared to urea, CRU reduced nitrogen input by 18.41~20.47% while achieving equivalent or higher theoretical yields. This study provides a scientific basis for nitrogen status diagnosis and precision nitrogen application in alfalfa cultivation.
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spelling doaj-art-0e9a35d3aee94c238841cb396e66fd462025-08-20T03:27:36ZengMDPI AGPlants2223-77472025-06-011412178210.3390/plants14121782Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve ModelYaya Duan0Yi Ling1Haiyan Li2Wenjing Chang3Jiandong Lu4Minhua Yin5Yanxia Kang6Yanlin Ma7Yayu Wang8Guangping Qi9Guoyun Shen10College of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Water Conservancy and Hydrpower Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaThe critical nitrogen dilution curve (CNDC) model enables precise nitrogen management by quantifying the threshold of nitrogen deficiency in crops, thereby enhancing both crop productivity and nitrogen use efficiency. However, its applicability to perennial crops remains unclear. In this study, alfalfa (<i>Medicago sativa</i> L.), a perennial leguminous forage, was used as the model crop. Based on two years of field experiments, CNDC models of aboveground biomass were constructed under two nitrogen fertilizer regimes: urea (0, 80, 160, and 240 kg·ha<sup>−1</sup>, applied in a 6:2:2 basal-to-topdressing ratio) and controlled-release urea (CRU; 0, 80, 160, and 240 kg·ha<sup>−1</sup>, applied as a single basal dose). Using these models, the nitrogen nutrition index (NNI) and cumulative nitrogen deficit (N<sub>and</sub>) models were developed to diagnose alfalfa nitrogen status, and the optimal nitrogen application rates were determined via regression analysis. The results showed that critical nitrogen concentration and aboveground biomass followed a power function relationship under both fertilizer types. For CRU treatments, parameters a and b were 3.41 and 0.20 (first cut), 3.15 and 0.12 (second cut), and 2.24 and 0.40 (third cut), respectively. For urea treatments, a and b were 3.13 and 0.35 (first cut), 2.21 and 0.16 (second cut), and 1.75 and 0.73 (third cut). The normalized root mean square error (n-RMSE) of the models ranged from 3.1% to 13%, indicating high model reliability. Based on the NNI, N<sub>and</sub>, and yield response models, the optimal nitrogen application rates were 175.44~181.71 kg·ha<sup>−1</sup> for urea and 145.63~153.46 kg·ha<sup>−1</sup> for CRU, corresponding to theoretical maximum yields of 14.76~17.40 t·ha<sup>−1</sup> and 16.76~20.66 t·ha<sup>−1</sup>, respectively. Compared to urea, CRU reduced nitrogen input by 18.41~20.47% while achieving equivalent or higher theoretical yields. This study provides a scientific basis for nitrogen status diagnosis and precision nitrogen application in alfalfa cultivation.https://www.mdpi.com/2223-7747/14/12/1782critical nitrogen concentrationnitrogen nutrition diagnosticsnitrogen application ratesalfalfa
spellingShingle Yaya Duan
Yi Ling
Haiyan Li
Wenjing Chang
Jiandong Lu
Minhua Yin
Yanxia Kang
Yanlin Ma
Yayu Wang
Guangping Qi
Guoyun Shen
Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
Plants
critical nitrogen concentration
nitrogen nutrition diagnostics
nitrogen application rates
alfalfa
title Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
title_full Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
title_fullStr Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
title_full_unstemmed Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
title_short Optimizing Management of Alfalfa (<i>Medicago sativa</i> L.) Nitrogen Fertilizer Based on Critical Nitrogen Concentration Dilution Curve Model
title_sort optimizing management of alfalfa i medicago sativa i l nitrogen fertilizer based on critical nitrogen concentration dilution curve model
topic critical nitrogen concentration
nitrogen nutrition diagnostics
nitrogen application rates
alfalfa
url https://www.mdpi.com/2223-7747/14/12/1782
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