Mechanism Underlying <i>Ganoderma lucidum</i> Polysaccharide Biosynthesis Regulation by the <i>β</i>-1,3-Glucosyltransferase Gene <i>gl20535</i>
<i>Ganoderma lucidum</i> polysaccharides (GLPs) are natural compounds with a broad spectrum of biological activities. <i>β</i>-1,3-glucosyltransferase (GL20535) plays an important role in polysaccharide synthesis by catalyzing the transfer of UDP-glucose to extend sugar chain...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/7/532 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Ganoderma lucidum</i> polysaccharides (GLPs) are natural compounds with a broad spectrum of biological activities. <i>β</i>-1,3-glucosyltransferase (GL20535) plays an important role in polysaccharide synthesis by catalyzing the transfer of UDP-glucose to extend sugar chains, but its underlying mechanism remains unclear. In this study, the regulatory mechanism of GL20535 in polysaccharide synthesis was elucidated by overexpressing and silencing <i>gl20535</i> in <i>G. lucidum</i>. Overexpression of <i>gl20535</i> resulted in maximum increases of 18.08%, 79.04%, and 18.01% in intracellular polysaccharide (IPS), extracellular polysaccharide (EPS), and <i>β</i>-1,3-glucan contents, respectively. In contrast, silencing <i>gl20535</i> resulted in maximum reductions of 16.97%, 30.20%, and 23.56% in IPS, EPS, and <i>β</i>-1,3-glucan contents, respectively. These phenomena in the overexpression strains were attributed to <i>gl20535</i>-mediated promotion of UDP-glucose synthesis in the sugar donor pathway and upregulation of the expression of glycoside hydrolase genes. The opposite trend was observed in the silenced strains. In mycelial growth studies, neither overexpression nor silencing of <i>gl20535</i> affected biomass and cell wall thickness. Furthermore, the GL20535 isozyme gene <i>gl24465</i> remained unaffected in <i>gl20535</i>-overexpressed strains but was upregulated in <i>gl20535</i>-silenced strains, suggesting a compensatory regulatory relationship. These findings reveal the regulatory role of GL20535 on gene expression in the GLPs synthesis pathway and deepen our understanding of GL20535 function in the polysaccharide network of edible and medicinal fungi. |
|---|---|
| ISSN: | 2309-608X |