Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear....
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1267480 |
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| author | Kaisaier Aji Munila Maimaijiang Abudusaimi Aimaiti Mulati Rexiati Baihetiya Azhati Hamulati Tusong Lei Cui |
| author_facet | Kaisaier Aji Munila Maimaijiang Abudusaimi Aimaiti Mulati Rexiati Baihetiya Azhati Hamulati Tusong Lei Cui |
| author_sort | Kaisaier Aji |
| collection | DOAJ |
| description | The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs. |
| format | Article |
| id | doaj-art-0e8b63bc7cdb4bcea28902253ba658db |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0e8b63bc7cdb4bcea28902253ba658db2025-08-20T02:01:40ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/12674801267480Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγKaisaier Aji0Munila Maimaijiang1Abudusaimi Aimaiti2Mulati Rexiati3Baihetiya Azhati4Hamulati Tusong5Lei Cui6Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaPrevention and Health Care Department, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830063, ChinaDepartment of Joint Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, ChinaThe multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.http://dx.doi.org/10.1155/2016/1267480 |
| spellingShingle | Kaisaier Aji Munila Maimaijiang Abudusaimi Aimaiti Mulati Rexiati Baihetiya Azhati Hamulati Tusong Lei Cui Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ Stem Cells International |
| title | Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ |
| title_full | Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ |
| title_fullStr | Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ |
| title_full_unstemmed | Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ |
| title_short | Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ |
| title_sort | differentiation of human adipose derived stem cells into smooth muscle cells is modulated by camkiiγ |
| url | http://dx.doi.org/10.1155/2016/1267480 |
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