New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia
The thermophilic fungus Penicillium species was isolated from Ghamiqa hot spring sediments in Saudi Arabia. Extract of Penicillium species cultured on solid rice medium yielded two new compounds 3-(furan 12-carboxylic acid)-6-(methoxycarbonyl)-4-hydroxy-4-methyl-4 and 5-dihydro-2H-pyran 1 3α-methyl-...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7162948 |
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| author | Raha Orfali Shagufta Perveen |
| author_facet | Raha Orfali Shagufta Perveen |
| author_sort | Raha Orfali |
| collection | DOAJ |
| description | The thermophilic fungus Penicillium species was isolated from Ghamiqa hot spring sediments in Saudi Arabia. Extract of Penicillium species cultured on solid rice medium yielded two new compounds 3-(furan 12-carboxylic acid)-6-(methoxycarbonyl)-4-hydroxy-4-methyl-4 and 5-dihydro-2H-pyran 1 3α-methyl-7-hydroxy-5-carboxylic acid methyl ester-1-indanone 2. In addition, three known compounds, austinol 3, emodin 4, and 2-methyl-penicinoline 5, were isolated. The structures of the new compounds were unambiguously determined by comprehensive analysis of spectroscopic data, one- and two-dimensional NMR spectroscopy, and high-resolution mass spectrometry. All isolated metabolites were studied for their antibiotic effect against several pathogenic bacteria and for their cytotoxicity against the lymphoma human cancer cell line HTB-176. Austinol 3 exhibited strong antibacterial activity against P. aeruginosa bacterial strain with an MIC value of 0.13 ± 0.4 µg·mL−1, whereas emodin 4 demonstrated significant cytotoxicity against the tested HTB-176 cell line with an IC50 value of 2 ± 7.6 µM, while the other compounds were moderate to inactive with IC50 ranging from 4.3 ± 0.25–22 ± 2.94 µM in this assay. |
| format | Article |
| id | doaj-art-0e85a5237cc04857bd841d3bf8f2b395 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-0e85a5237cc04857bd841d3bf8f2b3952025-02-03T01:27:39ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/71629487162948New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi ArabiaRaha Orfali0Shagufta Perveen1Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaThe thermophilic fungus Penicillium species was isolated from Ghamiqa hot spring sediments in Saudi Arabia. Extract of Penicillium species cultured on solid rice medium yielded two new compounds 3-(furan 12-carboxylic acid)-6-(methoxycarbonyl)-4-hydroxy-4-methyl-4 and 5-dihydro-2H-pyran 1 3α-methyl-7-hydroxy-5-carboxylic acid methyl ester-1-indanone 2. In addition, three known compounds, austinol 3, emodin 4, and 2-methyl-penicinoline 5, were isolated. The structures of the new compounds were unambiguously determined by comprehensive analysis of spectroscopic data, one- and two-dimensional NMR spectroscopy, and high-resolution mass spectrometry. All isolated metabolites were studied for their antibiotic effect against several pathogenic bacteria and for their cytotoxicity against the lymphoma human cancer cell line HTB-176. Austinol 3 exhibited strong antibacterial activity against P. aeruginosa bacterial strain with an MIC value of 0.13 ± 0.4 µg·mL−1, whereas emodin 4 demonstrated significant cytotoxicity against the tested HTB-176 cell line with an IC50 value of 2 ± 7.6 µM, while the other compounds were moderate to inactive with IC50 ranging from 4.3 ± 0.25–22 ± 2.94 µM in this assay.http://dx.doi.org/10.1155/2019/7162948 |
| spellingShingle | Raha Orfali Shagufta Perveen New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia Journal of Chemistry |
| title | New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia |
| title_full | New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia |
| title_fullStr | New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia |
| title_full_unstemmed | New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia |
| title_short | New Bioactive Metabolites from the Thermophilic Fungus Penicillium sp. Isolated from Ghamiqa Hot Spring in Saudi Arabia |
| title_sort | new bioactive metabolites from the thermophilic fungus penicillium sp isolated from ghamiqa hot spring in saudi arabia |
| url | http://dx.doi.org/10.1155/2019/7162948 |
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