Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography
In this paper, isomaltooligosaccharides 50 syrup was used as raw material and purification of isomaltooligosaccharides by sequential simulated moving bed chromatography, and the related physical and chemical properties of high-purity isomaltooligosaccharide were analyzed. The stationary phase suitab...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2025-02-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040407 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823865186689220608 |
|---|---|
| author | Shenghui RUAN Xiqun ZHENG Xiaolan LIU Liangyu LI |
| author_facet | Shenghui RUAN Xiqun ZHENG Xiaolan LIU Liangyu LI |
| author_sort | Shenghui RUAN |
| collection | DOAJ |
| description | In this paper, isomaltooligosaccharides 50 syrup was used as raw material and purification of isomaltooligosaccharides by sequential simulated moving bed chromatography, and the related physical and chemical properties of high-purity isomaltooligosaccharide were analyzed. The stationary phase suitable for the separation of isomaltooligosaccharides was selected by single column fixed bed experiment. The separation degree of different types of cation exchange resin on isomaltooligosaccharides, monosaccharides and disaccharides mixture was compared, ZG106 K+ resin was selected as the suitable separation medium. Then, based on the single column evaluation test of preparative chromatography and the principle of material balance and the basic principle of sequential simulated moving bed. The initial operating parameters for the sequential simulated moving bed chromatographic separation high-purity isomaltooligosaccharides process were roughly determined. Further the separation conditions were optimized and the best technical parameters were as follows: feed refraction was 60% and column temperature was 65 ℃. At the same time quantity of flow in the entrance and eluent were 455 mL/h and 682 mL/h, respectively. The velocity of flow in the entrance and eluent were 40.2 mL/min and 31.8 mL/min, respectively. Besides, the circulating flow was kept within 409.5 mL/h. Finally, the outlet refraction of isomaltooligosaccharides was 18.70%. Products gained with purity was 92.80% and the yield was 83.20%. After 7 days of storage in environments with relative humidity of 43% and 81%, the moisture absorption rates of high-purity isomaltooligosaccharides were 61.63% and 67.10% respectively. Meanwhile, after 7 days of storage in environments with relative humidity of 43% and 0, the moisture retention rates of high-purity isomaltooligosaccharides were 77.96% and 15.56% respectively. Its chemical structure could still maintain high stability at 352.5 ℃. It was capable of performing the Maillard reaction. Syrup was a typical non-Newtonian "pseudoplastic fluid". Sequential simulated moving bed technology can efficiently purify isomaltooligosaccharides, which provides corresponding technical support for the large-scale commercial production and wide application of high-purity isomaltooligosaccharides. The good physical and chemical properties of high purity isomaltooligosaccharide provide a theoretical basis for its application in food and related functional sugar fields. |
| format | Article |
| id | doaj-art-0e7360344d244f31ae45b13f67f0b5bf |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-0e7360344d244f31ae45b13f67f0b5bf2025-02-08T08:06:21ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146422523610.13386/j.issn1002-0306.20240404072024040407-4Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed ChromatographyShenghui RUAN0Xiqun ZHENG1Xiaolan LIU2Liangyu LI3College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaHeilongjiang Key Laboratory of Corn Deep Processing Theory and Technology, College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University,Daqing 163319, ChinaIn this paper, isomaltooligosaccharides 50 syrup was used as raw material and purification of isomaltooligosaccharides by sequential simulated moving bed chromatography, and the related physical and chemical properties of high-purity isomaltooligosaccharide were analyzed. The stationary phase suitable for the separation of isomaltooligosaccharides was selected by single column fixed bed experiment. The separation degree of different types of cation exchange resin on isomaltooligosaccharides, monosaccharides and disaccharides mixture was compared, ZG106 K+ resin was selected as the suitable separation medium. Then, based on the single column evaluation test of preparative chromatography and the principle of material balance and the basic principle of sequential simulated moving bed. The initial operating parameters for the sequential simulated moving bed chromatographic separation high-purity isomaltooligosaccharides process were roughly determined. Further the separation conditions were optimized and the best technical parameters were as follows: feed refraction was 60% and column temperature was 65 ℃. At the same time quantity of flow in the entrance and eluent were 455 mL/h and 682 mL/h, respectively. The velocity of flow in the entrance and eluent were 40.2 mL/min and 31.8 mL/min, respectively. Besides, the circulating flow was kept within 409.5 mL/h. Finally, the outlet refraction of isomaltooligosaccharides was 18.70%. Products gained with purity was 92.80% and the yield was 83.20%. After 7 days of storage in environments with relative humidity of 43% and 81%, the moisture absorption rates of high-purity isomaltooligosaccharides were 61.63% and 67.10% respectively. Meanwhile, after 7 days of storage in environments with relative humidity of 43% and 0, the moisture retention rates of high-purity isomaltooligosaccharides were 77.96% and 15.56% respectively. Its chemical structure could still maintain high stability at 352.5 ℃. It was capable of performing the Maillard reaction. Syrup was a typical non-Newtonian "pseudoplastic fluid". Sequential simulated moving bed technology can efficiently purify isomaltooligosaccharides, which provides corresponding technical support for the large-scale commercial production and wide application of high-purity isomaltooligosaccharides. The good physical and chemical properties of high purity isomaltooligosaccharide provide a theoretical basis for its application in food and related functional sugar fields.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040407isomaltooligosaccharidessequential simulated moving bed chromatographypreparative chromatographypurificationphysicochemical property |
| spellingShingle | Shenghui RUAN Xiqun ZHENG Xiaolan LIU Liangyu LI Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography Shipin gongye ke-ji isomaltooligosaccharides sequential simulated moving bed chromatography preparative chromatography purification physicochemical property |
| title | Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography |
| title_full | Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography |
| title_fullStr | Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography |
| title_full_unstemmed | Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography |
| title_short | Preparation and Physicochemical Properties of High-purity Isomaltooligosaccharides by Sequential Simulated Moving Bed Chromatography |
| title_sort | preparation and physicochemical properties of high purity isomaltooligosaccharides by sequential simulated moving bed chromatography |
| topic | isomaltooligosaccharides sequential simulated moving bed chromatography preparative chromatography purification physicochemical property |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040407 |
| work_keys_str_mv | AT shenghuiruan preparationandphysicochemicalpropertiesofhighpurityisomaltooligosaccharidesbysequentialsimulatedmovingbedchromatography AT xiqunzheng preparationandphysicochemicalpropertiesofhighpurityisomaltooligosaccharidesbysequentialsimulatedmovingbedchromatography AT xiaolanliu preparationandphysicochemicalpropertiesofhighpurityisomaltooligosaccharidesbysequentialsimulatedmovingbedchromatography AT liangyuli preparationandphysicochemicalpropertiesofhighpurityisomaltooligosaccharidesbysequentialsimulatedmovingbedchromatography |