Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA
ABSTRACT: Objectives: Staphylococcus aureus (S. aureus) is a major cause of bloodstream infections. The recent epidemiological features and antimicrobial resistance trend were analysed for methicillin-resistant and susceptible S. aureus (MRSA/MSSA) isolates from blood samples in people from norther...
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Elsevier
2025-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004727 |
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| author | Meiji Soe Aung Masako Osada Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Masahiko Ito Kazuki Yamada Kousuke Tada Nobumichi Kobayashi |
| author_facet | Meiji Soe Aung Masako Osada Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Masahiko Ito Kazuki Yamada Kousuke Tada Nobumichi Kobayashi |
| author_sort | Meiji Soe Aung |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Staphylococcus aureus (S. aureus) is a major cause of bloodstream infections. The recent epidemiological features and antimicrobial resistance trend were analysed for methicillin-resistant and susceptible S. aureus (MRSA/MSSA) isolates from blood samples in people from northern Japan. Methods: The S. aureus isolates from blood culture were screened by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and genotyped by the schemes of multilocus sequence typing (MLST), coa, agr, spa, and SCCmec types. Antimicrobial resistance genes and virulence factors were detected by multiplex/uniplex polymerase chain reaction (PCR). Antimicrobial susceptibility was examined using a broth microdilution test. Results: A total of 301 isolates (163 MRSA and 138 MSSA) were isolated from bloodstream infections in 2023 (from April to December). The MRSA isolates were classified into three groups, that is, clonal complexes (CC)1-SCCmec-IV (CC1-IV) (52%), CC5-II (36%), and CC8-IV (12%). The prevalence of CC1 was significantly higher than those in our previous studies (2017–2021). Four CC8-IVa isolates with PVL genes on ΦSa2usa were considered to be the USA300 clone (sequence type [ST]8/spa-t008/coa IIIa/agr I) or its variants that were genotyped as those closely related to ST8/t008 or lacking arginine catabolic mobile element (ACME). In contrast, MSSA was genetically highly divergent and classified into 22 STs, with CC1 (ST1 and ST188) being the most common (25%). It was notable that 29 MSSA isolates (21%) were classified into livestock-associated (LA) genotypes, ST20, ST97, and CC398 (ST398 and ST291). Genetic characterization of the CC398 isolates suggested that these belong to human-adapted MSSA clones. Conclusions: The present study revealed the increasing trend of CC1 MRSA surpassing CC5, and the emergence of MSSA representing human-adapted CC398, and LA types ST97 and ST20 from bloodstream infections in people in Japan. © 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. |
| format | Article |
| id | doaj-art-0e47f31e025d4d6bb9748df5b3ea6962 |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-0e47f31e025d4d6bb9748df5b3ea69622025-08-20T01:57:35ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-03-0141778710.1016/j.jgar.2024.12.010Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSAMeiji Soe Aung0Masako Osada1Noriko Urushibara2Mitsuyo Kawaguchiya3Nobuhide Ohashi4Mina Hirose5Masahiko Ito6Kazuki Yamada7Kousuke Tada8Nobumichi Kobayashi9Department of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, Japan; Corresponding author. Mailing address: Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo 060-8556, Japan.Master's Program in Midwifery, Tenshi College Graduate School, Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanDivision of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, JapanSapporo Clinical Laboratory, Inc., Sapporo, JapanSapporo Clinical Laboratory, Inc., Sapporo, JapanSapporo Clinical Laboratory, Inc., Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Hokkaido, Sapporo, JapanABSTRACT: Objectives: Staphylococcus aureus (S. aureus) is a major cause of bloodstream infections. The recent epidemiological features and antimicrobial resistance trend were analysed for methicillin-resistant and susceptible S. aureus (MRSA/MSSA) isolates from blood samples in people from northern Japan. Methods: The S. aureus isolates from blood culture were screened by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and genotyped by the schemes of multilocus sequence typing (MLST), coa, agr, spa, and SCCmec types. Antimicrobial resistance genes and virulence factors were detected by multiplex/uniplex polymerase chain reaction (PCR). Antimicrobial susceptibility was examined using a broth microdilution test. Results: A total of 301 isolates (163 MRSA and 138 MSSA) were isolated from bloodstream infections in 2023 (from April to December). The MRSA isolates were classified into three groups, that is, clonal complexes (CC)1-SCCmec-IV (CC1-IV) (52%), CC5-II (36%), and CC8-IV (12%). The prevalence of CC1 was significantly higher than those in our previous studies (2017–2021). Four CC8-IVa isolates with PVL genes on ΦSa2usa were considered to be the USA300 clone (sequence type [ST]8/spa-t008/coa IIIa/agr I) or its variants that were genotyped as those closely related to ST8/t008 or lacking arginine catabolic mobile element (ACME). In contrast, MSSA was genetically highly divergent and classified into 22 STs, with CC1 (ST1 and ST188) being the most common (25%). It was notable that 29 MSSA isolates (21%) were classified into livestock-associated (LA) genotypes, ST20, ST97, and CC398 (ST398 and ST291). Genetic characterization of the CC398 isolates suggested that these belong to human-adapted MSSA clones. Conclusions: The present study revealed the increasing trend of CC1 MRSA surpassing CC5, and the emergence of MSSA representing human-adapted CC398, and LA types ST97 and ST20 from bloodstream infections in people in Japan. © 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.http://www.sciencedirect.com/science/article/pii/S2213716524004727Methicillin-resistant Staphylococcus aureus (MRSA)Methicillin-susceptible Staphylococcus aureus (MSSA)Bloodstream infectionSequence type (ST)Livestock-associated (LA) genotypeJapan |
| spellingShingle | Meiji Soe Aung Masako Osada Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Masahiko Ito Kazuki Yamada Kousuke Tada Nobumichi Kobayashi Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA Journal of Global Antimicrobial Resistance Methicillin-resistant Staphylococcus aureus (MRSA) Methicillin-susceptible Staphylococcus aureus (MSSA) Bloodstream infection Sequence type (ST) Livestock-associated (LA) genotype Japan |
| title | Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA |
| title_full | Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA |
| title_fullStr | Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA |
| title_full_unstemmed | Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA |
| title_short | Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA |
| title_sort | molecular characterization of methicillin susceptible resistant staphylococcus aureus from bloodstream infections in northern japan the dominance of cc1 mrsa iv the emergence of human associated st398 and livestock associated cc20 and cc97 mssa |
| topic | Methicillin-resistant Staphylococcus aureus (MRSA) Methicillin-susceptible Staphylococcus aureus (MSSA) Bloodstream infection Sequence type (ST) Livestock-associated (LA) genotype Japan |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004727 |
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