Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions
Studying the physiological mechanisms underlying the traits associated with salt tolerance in genotypes could lead to the discovery of new genetic resources for salt tolerance. In this study, the mechanisms of salt tolerance were evaluated, based on ionic, physiological, and agronomic traits in four...
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2024-11-01
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| author | Muhammad Usman Tahir Salah El-Hendawy Nasser Al-Suhaibani |
| author_facet | Muhammad Usman Tahir Salah El-Hendawy Nasser Al-Suhaibani |
| author_sort | Muhammad Usman Tahir |
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| description | Studying the physiological mechanisms underlying the traits associated with salt tolerance in genotypes could lead to the discovery of new genetic resources for salt tolerance. In this study, the mechanisms of salt tolerance were evaluated, based on ionic, physiological, and agronomic traits in four varieties that differ in their salt tolerance and in 18 F<sub>8</sub> recombinant inbred lines (RILs) grown in real field conditions. The salt tolerance of plant materials was assessed under both normal (3.5 mM NaCl) and high salinity stress (150 mM NaCl) conditions for two consecutive years. Different growth and physiological traits were assessed 75 days after sowing, while ion contents in the shoots, grain yield, and its components were determined at the maturity stage. Multivariate analysis was used to conduct a comprehensive evaluation of salt tolerance across various genotypes and traits. The ANOVA results showed significant differences (<i>p</i> ≤ 0.05 and 0.001) among salinity, genotypes, and their interactions for all ionic and agro-physiological traits, with a few exceptions. Salinity stress resulted in a considerable increase in Na<sup>+</sup> content and canopy temperature (CT), with a simultaneous decrease of 11.3% to 94.5% in other ionic and agro-physiological traits compared to the control treatment. However, the salt-tolerant genotypes showed minimal increases in Na<sup>+</sup> content and CT, as well as decreases in other ionic and agro-physiological traits when compared to salt-sensitive genotypes under salinity stress. All ionic and agro-physiological traits exhibited strong correlations with each other under salinity stress, but these correlations were weak or insignificant under control conditions. The principal component analysis identified Na<sup>+</sup> and CT as negative indicators and other ionic and agro-physiological traits as positive indicators for salt tolerance under salinity stress. The negative indicators were strongly linked to salt-sensitive genotypes, while the positive indicators were closely associated with salt-tolerant genotypes. Heatmap clustering, using multiple traits, successfully differentiated the salt-tolerant genotypes from the salt-sensitive ones. The salt-tolerant group showed a significant reduction in Na<sup>+</sup> content by 36.9%, in CT by 10.0%, and in HI by 16.7%, along with an increase of 6.3–51.4% in other ionic and agro-physiological traits compared to the salt-sensitive group. In conclusion, the mechanisms associated with Na<sup>+</sup> exclusion and high K<sup>+</sup>/Na<sup>+</sup> and Ca<sup>2+</sup>/Na<sup>+</sup> ratios, as well as chlorophyll and relative water content, along with low CT, resulted in significant improvements in growth and yield under salinity stress conditions. Given that the effectiveness of various ionic and agro-physiological traits in evaluating salt tolerance in wheat has been proven in real field conditions, these traits will play a key role in the development of salt-tolerant wheat genotypes. |
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| spelling | doaj-art-0e4602e33bea472da9b6cd1d34e436e92025-08-20T01:53:57ZengMDPI AGLife2075-17292024-11-011411148710.3390/life14111487Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field ConditionsMuhammad Usman Tahir0Salah El-Hendawy1Nasser Al-Suhaibani2Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, KSA, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Plant Production, College of Food and Agriculture Sciences, King Saud University, KSA, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Plant Production, College of Food and Agriculture Sciences, King Saud University, KSA, P.O. Box 2460, Riyadh 11451, Saudi ArabiaStudying the physiological mechanisms underlying the traits associated with salt tolerance in genotypes could lead to the discovery of new genetic resources for salt tolerance. In this study, the mechanisms of salt tolerance were evaluated, based on ionic, physiological, and agronomic traits in four varieties that differ in their salt tolerance and in 18 F<sub>8</sub> recombinant inbred lines (RILs) grown in real field conditions. The salt tolerance of plant materials was assessed under both normal (3.5 mM NaCl) and high salinity stress (150 mM NaCl) conditions for two consecutive years. Different growth and physiological traits were assessed 75 days after sowing, while ion contents in the shoots, grain yield, and its components were determined at the maturity stage. Multivariate analysis was used to conduct a comprehensive evaluation of salt tolerance across various genotypes and traits. The ANOVA results showed significant differences (<i>p</i> ≤ 0.05 and 0.001) among salinity, genotypes, and their interactions for all ionic and agro-physiological traits, with a few exceptions. Salinity stress resulted in a considerable increase in Na<sup>+</sup> content and canopy temperature (CT), with a simultaneous decrease of 11.3% to 94.5% in other ionic and agro-physiological traits compared to the control treatment. However, the salt-tolerant genotypes showed minimal increases in Na<sup>+</sup> content and CT, as well as decreases in other ionic and agro-physiological traits when compared to salt-sensitive genotypes under salinity stress. All ionic and agro-physiological traits exhibited strong correlations with each other under salinity stress, but these correlations were weak or insignificant under control conditions. The principal component analysis identified Na<sup>+</sup> and CT as negative indicators and other ionic and agro-physiological traits as positive indicators for salt tolerance under salinity stress. The negative indicators were strongly linked to salt-sensitive genotypes, while the positive indicators were closely associated with salt-tolerant genotypes. Heatmap clustering, using multiple traits, successfully differentiated the salt-tolerant genotypes from the salt-sensitive ones. The salt-tolerant group showed a significant reduction in Na<sup>+</sup> content by 36.9%, in CT by 10.0%, and in HI by 16.7%, along with an increase of 6.3–51.4% in other ionic and agro-physiological traits compared to the salt-sensitive group. In conclusion, the mechanisms associated with Na<sup>+</sup> exclusion and high K<sup>+</sup>/Na<sup>+</sup> and Ca<sup>2+</sup>/Na<sup>+</sup> ratios, as well as chlorophyll and relative water content, along with low CT, resulted in significant improvements in growth and yield under salinity stress conditions. Given that the effectiveness of various ionic and agro-physiological traits in evaluating salt tolerance in wheat has been proven in real field conditions, these traits will play a key role in the development of salt-tolerant wheat genotypes.https://www.mdpi.com/2075-1729/14/11/1487canopy temperaturechlorophyll contentheatmap clusteringmaximal PSII photochemical efficiencyNa<sup>+</sup> contentrelative water content |
| spellingShingle | Muhammad Usman Tahir Salah El-Hendawy Nasser Al-Suhaibani Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions Life canopy temperature chlorophyll content heatmap clustering maximal PSII photochemical efficiency Na<sup>+</sup> content relative water content |
| title | Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions |
| title_full | Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions |
| title_fullStr | Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions |
| title_full_unstemmed | Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions |
| title_short | Comparative Performance of Ionic and Agro-Physiological Traits for Detecting Salt Tolerance in Wheat Genotypes Grown in Real Field Conditions |
| title_sort | comparative performance of ionic and agro physiological traits for detecting salt tolerance in wheat genotypes grown in real field conditions |
| topic | canopy temperature chlorophyll content heatmap clustering maximal PSII photochemical efficiency Na<sup>+</sup> content relative water content |
| url | https://www.mdpi.com/2075-1729/14/11/1487 |
| work_keys_str_mv | AT muhammadusmantahir comparativeperformanceofionicandagrophysiologicaltraitsfordetectingsalttoleranceinwheatgenotypesgrowninrealfieldconditions AT salahelhendawy comparativeperformanceofionicandagrophysiologicaltraitsfordetectingsalttoleranceinwheatgenotypesgrowninrealfieldconditions AT nasseralsuhaibani comparativeperformanceofionicandagrophysiologicaltraitsfordetectingsalttoleranceinwheatgenotypesgrowninrealfieldconditions |