Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models

Abstract Background The continuing emergence of influenza virus has highlighted the value of public databases and related bioinformatic analysis tools in investigating transcriptomic change caused by different influenza virus infections in human and animal models. Methods We collected a large amount...

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Main Authors: Yue Wu, Jue Wang, Jing Xue, Zhiguang Xiang, Jianguo Guo, Lingjun Zhan, Qiang Wei, Qi Kong
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Animal Models and Experimental Medicine
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Online Access:https://doi.org/10.1002/ame2.12384
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author Yue Wu
Jue Wang
Jing Xue
Zhiguang Xiang
Jianguo Guo
Lingjun Zhan
Qiang Wei
Qi Kong
author_facet Yue Wu
Jue Wang
Jing Xue
Zhiguang Xiang
Jianguo Guo
Lingjun Zhan
Qiang Wei
Qi Kong
author_sort Yue Wu
collection DOAJ
description Abstract Background The continuing emergence of influenza virus has highlighted the value of public databases and related bioinformatic analysis tools in investigating transcriptomic change caused by different influenza virus infections in human and animal models. Methods We collected a large amount of transcriptome research data related to influenza virus‐infected human and animal models in public databases (GEO and ArrayExpress), and extracted and integrated array and metadata. The gene expression matrix was generated through strictly quality control, balance, standardization, batch correction, and gene annotation. We then analyzed gene expression in different species, virus, cells/tissues or after antibody/vaccine treatment and imported sample metadata and gene expression datasets into the database. Results Overall, maintaining careful processing and quality control, we collected 8064 samples from 103 independent datasets, and constructed a comparative transcriptomics database of influenza virus named the Flu‐CED database (Influenza comparative expression database, https://flu.com‐med.org.cn/). Using integrated and processed transcriptomic data, we established a user‐friendly website for realizing the integration, online retrieval, visualization, and exploration of gene expression of influenza virus infection in different species and the biological functions involved in differential genes. Flu‐CED can quickly query single and multi‐gene expression profiles, combining different experimental conditions for comparative transcriptome analysis, identifying differentially expressed genes (DEGs) between comparison groups, and conveniently finding DEGs. Conclusion Flu‐CED provides data resources and tools for analyzing gene expression in human and animal models infected with influenza virus that can deepen our understanding of the mechanisms underlying disease occurrence and development, and enable prediction of key genes or therapeutic targets that can be used for medical research.
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spelling doaj-art-0e38f9d580e34ac7b8b5c320d3c4df1e2025-08-20T02:50:55ZengWileyAnimal Models and Experimental Medicine2576-20952024-12-017688189210.1002/ame2.12384Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal modelsYue Wu0Jue Wang1Jing Xue2Zhiguang Xiang3Jianguo Guo4Lingjun Zhan5Qiang Wei6Qi Kong7Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, National Human Diseases Animal Model Resource Center, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Beijing ChinaAbstract Background The continuing emergence of influenza virus has highlighted the value of public databases and related bioinformatic analysis tools in investigating transcriptomic change caused by different influenza virus infections in human and animal models. Methods We collected a large amount of transcriptome research data related to influenza virus‐infected human and animal models in public databases (GEO and ArrayExpress), and extracted and integrated array and metadata. The gene expression matrix was generated through strictly quality control, balance, standardization, batch correction, and gene annotation. We then analyzed gene expression in different species, virus, cells/tissues or after antibody/vaccine treatment and imported sample metadata and gene expression datasets into the database. Results Overall, maintaining careful processing and quality control, we collected 8064 samples from 103 independent datasets, and constructed a comparative transcriptomics database of influenza virus named the Flu‐CED database (Influenza comparative expression database, https://flu.com‐med.org.cn/). Using integrated and processed transcriptomic data, we established a user‐friendly website for realizing the integration, online retrieval, visualization, and exploration of gene expression of influenza virus infection in different species and the biological functions involved in differential genes. Flu‐CED can quickly query single and multi‐gene expression profiles, combining different experimental conditions for comparative transcriptome analysis, identifying differentially expressed genes (DEGs) between comparison groups, and conveniently finding DEGs. Conclusion Flu‐CED provides data resources and tools for analyzing gene expression in human and animal models infected with influenza virus that can deepen our understanding of the mechanisms underlying disease occurrence and development, and enable prediction of key genes or therapeutic targets that can be used for medical research.https://doi.org/10.1002/ame2.12384animal modelcomparative analysisdatabasegene expressioninfluenza virus infection
spellingShingle Yue Wu
Jue Wang
Jing Xue
Zhiguang Xiang
Jianguo Guo
Lingjun Zhan
Qiang Wei
Qi Kong
Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
Animal Models and Experimental Medicine
animal model
comparative analysis
database
gene expression
influenza virus infection
title Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
title_full Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
title_fullStr Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
title_full_unstemmed Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
title_short Flu‐CED: A comparative transcriptomics database of influenza virus‐infected human and animal models
title_sort flu ced a comparative transcriptomics database of influenza virus infected human and animal models
topic animal model
comparative analysis
database
gene expression
influenza virus infection
url https://doi.org/10.1002/ame2.12384
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