Cross-species spill-over potential of the H9N2 bat influenza A virus

Abstract In 2017, a novel influenza A virus (IAV) was isolated from an Egyptian fruit bat. In contrast to other bat influenza viruses, the virus was related to avian A(H9N2) viruses and was probably the result of a bird-to-bat transmission event. To determine the cross-species spill-over potential,...

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Main Authors: Rabeh El-Shesheny, John Franks, Ahmed Kandeil, Rebecca Badra, Jasmine Turner, Patrick Seiler, Bindumadhav M. Marathe, Trushar Jeevan, Lisa Kercher, Meng Hu, Yul Eum Sim, Kenrie P. Y. Hui, Michael C. W. Chan, Andrew J. Thompson, Pamela McKenzie, Elena A. Govorkova, Charles J. Russell, Peter Vogel, James C. Paulson, J. S. Malik Peiris, Robert G. Webster, Mohamed A. Ali, Ghazi Kayali, Richard J. Webby
Format: Article
Language:English
Published: Nature Portfolio 2024-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-47635-4
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author Rabeh El-Shesheny
John Franks
Ahmed Kandeil
Rebecca Badra
Jasmine Turner
Patrick Seiler
Bindumadhav M. Marathe
Trushar Jeevan
Lisa Kercher
Meng Hu
Yul Eum Sim
Kenrie P. Y. Hui
Michael C. W. Chan
Andrew J. Thompson
Pamela McKenzie
Elena A. Govorkova
Charles J. Russell
Peter Vogel
James C. Paulson
J. S. Malik Peiris
Robert G. Webster
Mohamed A. Ali
Ghazi Kayali
Richard J. Webby
author_facet Rabeh El-Shesheny
John Franks
Ahmed Kandeil
Rebecca Badra
Jasmine Turner
Patrick Seiler
Bindumadhav M. Marathe
Trushar Jeevan
Lisa Kercher
Meng Hu
Yul Eum Sim
Kenrie P. Y. Hui
Michael C. W. Chan
Andrew J. Thompson
Pamela McKenzie
Elena A. Govorkova
Charles J. Russell
Peter Vogel
James C. Paulson
J. S. Malik Peiris
Robert G. Webster
Mohamed A. Ali
Ghazi Kayali
Richard J. Webby
author_sort Rabeh El-Shesheny
collection DOAJ
description Abstract In 2017, a novel influenza A virus (IAV) was isolated from an Egyptian fruit bat. In contrast to other bat influenza viruses, the virus was related to avian A(H9N2) viruses and was probably the result of a bird-to-bat transmission event. To determine the cross-species spill-over potential, we biologically characterize features of A/bat/Egypt/381OP/2017(H9N2). The virus has a pH inactivation profile and neuraminidase activity similar to those of human-adapted IAVs. Despite the virus having an avian virus–like preference for α2,3 sialic acid receptors, it is unable to replicate in male mallard ducks; however, it readily infects ex-vivo human respiratory cell cultures and replicates in the lungs of female mice. A/bat/Egypt/381OP/2017 replicates in the upper respiratory tract of experimentally-infected male ferrets featuring direct-contact and airborne transmission. These data suggest that the bat A(H9N2) virus has features associated with increased risk to humans without a shift to a preference for α2,6 sialic acid receptors.
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spelling doaj-art-0e383400a9d3481ebc14bb85730eae932025-08-20T02:34:19ZengNature PortfolioNature Communications2041-17232024-04-0115111210.1038/s41467-024-47635-4Cross-species spill-over potential of the H9N2 bat influenza A virusRabeh El-Shesheny0John Franks1Ahmed Kandeil2Rebecca Badra3Jasmine Turner4Patrick Seiler5Bindumadhav M. Marathe6Trushar Jeevan7Lisa Kercher8Meng Hu9Yul Eum Sim10Kenrie P. Y. Hui11Michael C. W. Chan12Andrew J. Thompson13Pamela McKenzie14Elena A. Govorkova15Charles J. Russell16Peter Vogel17James C. Paulson18J. S. Malik Peiris19Robert G. Webster20Mohamed A. Ali21Ghazi Kayali22Richard J. Webby23Center of Scientific Excellence for Influenza Virus, National Research CentreDepartment of Infectious Diseases, St. Jude Children’s Research HospitalCenter of Scientific Excellence for Influenza Virus, National Research CentreHuman LinkDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Biology, Wanek School of Natural Science, High Point UniversitySchool of Public Health, The University of Hong KongSchool of Public Health, The University of Hong KongDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Infectious Diseases, St. Jude Children’s Research HospitalDepartment of Immunology and Microbiology, The Scripps Research InstituteSchool of Public Health, The University of Hong KongDepartment of Infectious Diseases, St. Jude Children’s Research HospitalCenter of Scientific Excellence for Influenza Virus, National Research CentreHuman LinkDepartment of Infectious Diseases, St. Jude Children’s Research HospitalAbstract In 2017, a novel influenza A virus (IAV) was isolated from an Egyptian fruit bat. In contrast to other bat influenza viruses, the virus was related to avian A(H9N2) viruses and was probably the result of a bird-to-bat transmission event. To determine the cross-species spill-over potential, we biologically characterize features of A/bat/Egypt/381OP/2017(H9N2). The virus has a pH inactivation profile and neuraminidase activity similar to those of human-adapted IAVs. Despite the virus having an avian virus–like preference for α2,3 sialic acid receptors, it is unable to replicate in male mallard ducks; however, it readily infects ex-vivo human respiratory cell cultures and replicates in the lungs of female mice. A/bat/Egypt/381OP/2017 replicates in the upper respiratory tract of experimentally-infected male ferrets featuring direct-contact and airborne transmission. These data suggest that the bat A(H9N2) virus has features associated with increased risk to humans without a shift to a preference for α2,6 sialic acid receptors.https://doi.org/10.1038/s41467-024-47635-4
spellingShingle Rabeh El-Shesheny
John Franks
Ahmed Kandeil
Rebecca Badra
Jasmine Turner
Patrick Seiler
Bindumadhav M. Marathe
Trushar Jeevan
Lisa Kercher
Meng Hu
Yul Eum Sim
Kenrie P. Y. Hui
Michael C. W. Chan
Andrew J. Thompson
Pamela McKenzie
Elena A. Govorkova
Charles J. Russell
Peter Vogel
James C. Paulson
J. S. Malik Peiris
Robert G. Webster
Mohamed A. Ali
Ghazi Kayali
Richard J. Webby
Cross-species spill-over potential of the H9N2 bat influenza A virus
Nature Communications
title Cross-species spill-over potential of the H9N2 bat influenza A virus
title_full Cross-species spill-over potential of the H9N2 bat influenza A virus
title_fullStr Cross-species spill-over potential of the H9N2 bat influenza A virus
title_full_unstemmed Cross-species spill-over potential of the H9N2 bat influenza A virus
title_short Cross-species spill-over potential of the H9N2 bat influenza A virus
title_sort cross species spill over potential of the h9n2 bat influenza a virus
url https://doi.org/10.1038/s41467-024-47635-4
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