Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice
Abstract Skeletal muscle has a high regenerative ability and maintains homeostasis by rapidly regenerating from frequent damage caused by intense exercise or trauma. In sports, skeletal muscle damage occurs frequently due to intense exercise, so practical methods to promote skeletal muscle regenerat...
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| Format: | Article |
| Language: | English |
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Wiley
2024-10-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70092 |
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| author | Tomohiro Iwata Takanaga Shirai Kazuki Uemichi Riku Tanimura Tohru Takemasa |
| author_facet | Tomohiro Iwata Takanaga Shirai Kazuki Uemichi Riku Tanimura Tohru Takemasa |
| author_sort | Tomohiro Iwata |
| collection | DOAJ |
| description | Abstract Skeletal muscle has a high regenerative ability and maintains homeostasis by rapidly regenerating from frequent damage caused by intense exercise or trauma. In sports, skeletal muscle damage occurs frequently due to intense exercise, so practical methods to promote skeletal muscle regeneration are required. Recent studies have shown that it may be possible to promote skeletal muscle regeneration through new pathways, such as promoting autophagy and improving mitochondrial function. Spermidine is a type of polyamine, and oral intake of spermidine promotes autophagy and improves mitochondrial function without inhibiting mTOR. Therefore, we evaluate the effects of spermidine intake on skeletal muscle regeneration after injury using a mouse model of cardiotoxin‐induced muscle injury. Our results showed no significant change in skeletal muscle wet weight with spermidine intake at all time points. In addition, although spermidine intake significantly increased the mean fiber cross‐sectional area 14 days after injury, these effects were not observed at other time points. In addition, we analyzed stem cells, autophagy, mTOR signaling, inflammation, and mitochondria, but no significant effects of spermidine intake were observed at almost all time points and protein expression levels. Therefore, spermidine intake does not affect skeletal muscle regeneration after chemical injury, and if there is any, it is very limited. |
| format | Article |
| id | doaj-art-0e364efdd3eb4b4bb3f334374ccfeacf |
| institution | OA Journals |
| issn | 2051-817X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-0e364efdd3eb4b4bb3f334374ccfeacf2025-08-20T02:12:03ZengWileyPhysiological Reports2051-817X2024-10-011220n/an/a10.14814/phy2.70092Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male miceTomohiro Iwata0Takanaga Shirai1Kazuki Uemichi2Riku Tanimura3Tohru Takemasa4Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki JapanJapan Society for Promotion Science Chiyoda Tokyo JapanJapan Society for Promotion Science Chiyoda Tokyo JapanGraduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki JapanInstitute of Health and Sport Sciences University of Tsukuba Tsukuba Ibaraki JapanAbstract Skeletal muscle has a high regenerative ability and maintains homeostasis by rapidly regenerating from frequent damage caused by intense exercise or trauma. In sports, skeletal muscle damage occurs frequently due to intense exercise, so practical methods to promote skeletal muscle regeneration are required. Recent studies have shown that it may be possible to promote skeletal muscle regeneration through new pathways, such as promoting autophagy and improving mitochondrial function. Spermidine is a type of polyamine, and oral intake of spermidine promotes autophagy and improves mitochondrial function without inhibiting mTOR. Therefore, we evaluate the effects of spermidine intake on skeletal muscle regeneration after injury using a mouse model of cardiotoxin‐induced muscle injury. Our results showed no significant change in skeletal muscle wet weight with spermidine intake at all time points. In addition, although spermidine intake significantly increased the mean fiber cross‐sectional area 14 days after injury, these effects were not observed at other time points. In addition, we analyzed stem cells, autophagy, mTOR signaling, inflammation, and mitochondria, but no significant effects of spermidine intake were observed at almost all time points and protein expression levels. Therefore, spermidine intake does not affect skeletal muscle regeneration after chemical injury, and if there is any, it is very limited.https://doi.org/10.14814/phy2.70092autophagymitochondriamTOR signalingskeletal muscle regenerationspermidine |
| spellingShingle | Tomohiro Iwata Takanaga Shirai Kazuki Uemichi Riku Tanimura Tohru Takemasa Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice Physiological Reports autophagy mitochondria mTOR signaling skeletal muscle regeneration spermidine |
| title | Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| title_full | Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| title_fullStr | Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| title_full_unstemmed | Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| title_short | Effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| title_sort | effect of spermidine intake on skeletal muscle regeneration after chemical injury in male mice |
| topic | autophagy mitochondria mTOR signaling skeletal muscle regeneration spermidine |
| url | https://doi.org/10.14814/phy2.70092 |
| work_keys_str_mv | AT tomohiroiwata effectofspermidineintakeonskeletalmuscleregenerationafterchemicalinjuryinmalemice AT takanagashirai effectofspermidineintakeonskeletalmuscleregenerationafterchemicalinjuryinmalemice AT kazukiuemichi effectofspermidineintakeonskeletalmuscleregenerationafterchemicalinjuryinmalemice AT rikutanimura effectofspermidineintakeonskeletalmuscleregenerationafterchemicalinjuryinmalemice AT tohrutakemasa effectofspermidineintakeonskeletalmuscleregenerationafterchemicalinjuryinmalemice |