BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration
IntroductionThe treatment of osteoporotic fractures is still challenging and may be improved using materials for bone regeneration, such as pre-structured gelatin with calcium and strontium phosphates (PPGC+S), combined with brain-derived neurotrophic factor (BDNF). Recently, it was shown that PPGC+...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1596846/full |
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| author | Paul T. Itting Paul T. Itting Benjamin Kruppke Thomas Hanke Vinu Vijayan Christian Heiss Christian Heiss Christian Heiss Katrin Susanne Lips |
| author_facet | Paul T. Itting Paul T. Itting Benjamin Kruppke Thomas Hanke Vinu Vijayan Christian Heiss Christian Heiss Christian Heiss Katrin Susanne Lips |
| author_sort | Paul T. Itting |
| collection | DOAJ |
| description | IntroductionThe treatment of osteoporotic fractures is still challenging and may be improved using materials for bone regeneration, such as pre-structured gelatin with calcium and strontium phosphates (PPGC+S), combined with brain-derived neurotrophic factor (BDNF). Recently, it was shown that PPGC+S stimulates bone formation, and BDNF improves cell survival. This study aimed to analyze the combined effect of PPGC+S and BDNF on the survival of mesenchymal stem cells (MSCs) from osteoporotic and non-osteoporotic female donors.MethodsIn our study, cells were seeded on PPGC+S plates with the following mineral composition of C+S of (a) 5:5, (b) 3:7, and (c) 0:10 (PPGS 0:10). Apoptosis and necrosis were measured after the addition of BDNF, followed by light microscopic analysis.ResultsThe application of BDNF resulted in reduced necrosis and apoptosis in all biomaterials. The lowest level of necrosis was found in the PPGC+S 5:5 group. Apoptosis was most reduced in the PPGC+S 3:7 group, although the difference compared to PPGC+S 5:5 was not statistically significant. No differences were observed between MSCs from osteoporotic and non-osteoporotic donors.ConclusionThus, PPGC+S 5:5 appears to be the most suitable composition for bone healing, especially when supplemented with BDNF. |
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| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-0e2c9de812234e4db087e697ac9b7c292025-08-20T03:28:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-07-011310.3389/fbioe.2025.15968461596846BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regenerationPaul T. Itting0Paul T. Itting1Benjamin Kruppke2Thomas Hanke3Vinu Vijayan4Christian Heiss5Christian Heiss6Christian Heiss7Katrin Susanne Lips8Department of Cardiovascular and Thoracic Surgery, University Medical Center Göttingen, Georg-August-University, Göttingen, GermanyExperimental Trauma Surgery, Justus-Liebig-University Giessen, Giessen, GermanyInstitute of Materials Science, Technische Universität Dresden, Max Bergmann Center of Biomaterials, Dresden, GermanyInstitute of Materials Science, Technische Universität Dresden, Max Bergmann Center of Biomaterials, Dresden, GermanyExperimental Trauma Surgery, Justus-Liebig-University Giessen, Giessen, GermanyExperimental Trauma Surgery, Justus-Liebig-University Giessen, Giessen, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital of Giessen-Marburg, Giessen, GermanyAdjunct Professor of Orthopedics, Biruni University, Istanbul, TürkiyeExperimental Trauma Surgery, Justus-Liebig-University Giessen, Giessen, GermanyIntroductionThe treatment of osteoporotic fractures is still challenging and may be improved using materials for bone regeneration, such as pre-structured gelatin with calcium and strontium phosphates (PPGC+S), combined with brain-derived neurotrophic factor (BDNF). Recently, it was shown that PPGC+S stimulates bone formation, and BDNF improves cell survival. This study aimed to analyze the combined effect of PPGC+S and BDNF on the survival of mesenchymal stem cells (MSCs) from osteoporotic and non-osteoporotic female donors.MethodsIn our study, cells were seeded on PPGC+S plates with the following mineral composition of C+S of (a) 5:5, (b) 3:7, and (c) 0:10 (PPGS 0:10). Apoptosis and necrosis were measured after the addition of BDNF, followed by light microscopic analysis.ResultsThe application of BDNF resulted in reduced necrosis and apoptosis in all biomaterials. The lowest level of necrosis was found in the PPGC+S 5:5 group. Apoptosis was most reduced in the PPGC+S 3:7 group, although the difference compared to PPGC+S 5:5 was not statistically significant. No differences were observed between MSCs from osteoporotic and non-osteoporotic donors.ConclusionThus, PPGC+S 5:5 appears to be the most suitable composition for bone healing, especially when supplemented with BDNF.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1596846/fullosteoporosisapoptosisbone regeneration materialcytotoxicitymesenchymal stem cellsbrain-derived neurotrophic factor |
| spellingShingle | Paul T. Itting Paul T. Itting Benjamin Kruppke Thomas Hanke Vinu Vijayan Christian Heiss Christian Heiss Christian Heiss Katrin Susanne Lips BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration Frontiers in Bioengineering and Biotechnology osteoporosis apoptosis bone regeneration material cytotoxicity mesenchymal stem cells brain-derived neurotrophic factor |
| title | BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| title_full | BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| title_fullStr | BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| title_full_unstemmed | BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| title_short | BDNF improves the survival of mesenchymal stem cells cultured on pre-structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| title_sort | bdnf improves the survival of mesenchymal stem cells cultured on pre structured gelatin material containing strontium and calcium phosphates for bone regeneration |
| topic | osteoporosis apoptosis bone regeneration material cytotoxicity mesenchymal stem cells brain-derived neurotrophic factor |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1596846/full |
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