Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice
Background. Multiple interleukin (IL) family members were reported to be closely related to hypertension. We aimed to investigate whether IL-9 affects angiotensin II- (Ang II-) induced hypertension in mice. Methods. Mice were treated with Ang II, and IL-9 expression was determined. In addition, effe...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/5741047 |
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| author | Yunzhao Yang Shaoqun Tang Chunchun Zhai Xin Zeng Qingjian Liu Cheng Xu Hexiang Chen |
| author_facet | Yunzhao Yang Shaoqun Tang Chunchun Zhai Xin Zeng Qingjian Liu Cheng Xu Hexiang Chen |
| author_sort | Yunzhao Yang |
| collection | DOAJ |
| description | Background. Multiple interleukin (IL) family members were reported to be closely related to hypertension. We aimed to investigate whether IL-9 affects angiotensin II- (Ang II-) induced hypertension in mice. Methods. Mice were treated with Ang II, and IL-9 expression was determined. In addition, effects of IL-9 knockout (KO) on blood pressure were observed in Ang II-infused mice. To determine whether the effects of IL-9 on blood pressure was mediated by the signal transducer and activator of the transcription 3 (STAT3) pathway, Ang II-treated mice were given S31-201. Furthermore, circulating IL-9 levels in patients with hypertension were measured. Results. Ang II treatment increased serum and aortic IL-9 expression in a dose-dependent manner; IL-9 levels were the highest in the second week and continued to remain high into the fourth week after the treatment. IL-9 KO downregulated proinflammatory cytokine expression, whereas it upregulated anti-inflammatory cytokine levels, relieved vascular dysfunction, and decreased blood pressure in Ang II-infused mice. IL-9 also reduced smooth muscle 22α (SM22α) expression and increased osteopontin (OPN) levels both in mice and in vitro. The effects of IL-9 KO on blood pressure and inflammatory response were significantly reduced by S31-201 treatment. Circulating IL-9 levels were significantly increased in patients with the hypertension group than in the control group, and elevated IL-9 levels positively correlated with both systolic blood pressure and diastolic blood pressure in patients with hypertension. Conclusions. IL-9 KO alleviates inflammatory response, prevents phenotypic transformation of smooth muscle, reduces vascular dysfunction, and lowers blood pressure via the STAT3 pathway in Ang II-infused mice. IL-9 might be a novel target for the treatment and prevention of clinical hypertension. |
| format | Article |
| id | doaj-art-0e0cbd1e98d141e2af79a43775803ef9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0e0cbd1e98d141e2af79a43775803ef92025-08-20T03:54:29ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/57410475741047Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated MiceYunzhao Yang0Shaoqun Tang1Chunchun Zhai2Xin Zeng3Qingjian Liu4Cheng Xu5Hexiang Chen6Department of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaDepartment of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, ChinaBackground. Multiple interleukin (IL) family members were reported to be closely related to hypertension. We aimed to investigate whether IL-9 affects angiotensin II- (Ang II-) induced hypertension in mice. Methods. Mice were treated with Ang II, and IL-9 expression was determined. In addition, effects of IL-9 knockout (KO) on blood pressure were observed in Ang II-infused mice. To determine whether the effects of IL-9 on blood pressure was mediated by the signal transducer and activator of the transcription 3 (STAT3) pathway, Ang II-treated mice were given S31-201. Furthermore, circulating IL-9 levels in patients with hypertension were measured. Results. Ang II treatment increased serum and aortic IL-9 expression in a dose-dependent manner; IL-9 levels were the highest in the second week and continued to remain high into the fourth week after the treatment. IL-9 KO downregulated proinflammatory cytokine expression, whereas it upregulated anti-inflammatory cytokine levels, relieved vascular dysfunction, and decreased blood pressure in Ang II-infused mice. IL-9 also reduced smooth muscle 22α (SM22α) expression and increased osteopontin (OPN) levels both in mice and in vitro. The effects of IL-9 KO on blood pressure and inflammatory response were significantly reduced by S31-201 treatment. Circulating IL-9 levels were significantly increased in patients with the hypertension group than in the control group, and elevated IL-9 levels positively correlated with both systolic blood pressure and diastolic blood pressure in patients with hypertension. Conclusions. IL-9 KO alleviates inflammatory response, prevents phenotypic transformation of smooth muscle, reduces vascular dysfunction, and lowers blood pressure via the STAT3 pathway in Ang II-infused mice. IL-9 might be a novel target for the treatment and prevention of clinical hypertension.http://dx.doi.org/10.1155/2020/5741047 |
| spellingShingle | Yunzhao Yang Shaoqun Tang Chunchun Zhai Xin Zeng Qingjian Liu Cheng Xu Hexiang Chen Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice Mediators of Inflammation |
| title | Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice |
| title_full | Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice |
| title_fullStr | Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice |
| title_full_unstemmed | Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice |
| title_short | Interleukin-9 Deletion Relieves Vascular Dysfunction and Decreases Blood Pressure via the STAT3 Pathway in Angiotensin II-Treated Mice |
| title_sort | interleukin 9 deletion relieves vascular dysfunction and decreases blood pressure via the stat3 pathway in angiotensin ii treated mice |
| url | http://dx.doi.org/10.1155/2020/5741047 |
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