Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer
Background. Colorectal cancer (CRC) is both a global and national burden, being the third most common malignancy in men and the second in women, worldwide. The prognosis of CRC is affected by various factors like the histological grade, angiolymphatic invasion, and distant metastases. Metastasis is...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2018/9613185 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563753128820736 |
|---|---|
| author | Naomi Yoshuantari Didik Setyo Heriyanto Susanna Hilda Hutajulu Johan Kurnianda Ahmad Ghozali |
| author_facet | Naomi Yoshuantari Didik Setyo Heriyanto Susanna Hilda Hutajulu Johan Kurnianda Ahmad Ghozali |
| author_sort | Naomi Yoshuantari |
| collection | DOAJ |
| description | Background. Colorectal cancer (CRC) is both a global and national burden, being the third most common malignancy in men and the second in women, worldwide. The prognosis of CRC is affected by various factors like the histological grade, angiolymphatic invasion, and distant metastases. Metastasis is an intricate process; one of the possible mechanisms is through the interaction of the chemokines CXCL12 and CXCR4. This study aims to reveal the expression patterns of CXCL12 and CXCR4 in CRC. Methods. The quantitative expressions of CXCL12 and CXCR4 messenger RNA (mRNA) were evaluated in 32 patients with adenocarcinoma-type CRC. Real-time polymerase chain reaction (qRT-PCR) was performed on formalin-fixed tissues. CXCL12 and CXCR4’s expressions, clinicopathologic features, and the treatment response to the CRC were analysed. Results. All tumour tissues showed higher levels of both chemokines compared to normal colonic tissue. The expression of CXCL12 mRNA was higher in rectal location (p=0.04) with a tendency to be higher in later stages (p=0.15), while the expression of CXCR4 was lower in tumours with a lymphatic invasion (p=0.02), compared to their counterparts. There was no difference in the expression of CXCL12 and CXCR4 according to the patients’ ages, gender, tumour differentiation, or response to chemotherapy. Conclusion. Our study demonstrated that the mRNA expression of CXCL12 was significantly correlated with rectal location. CXCR4 mRNA expression was inversely correlated in tumours with a lymphatic invasion. |
| format | Article |
| id | doaj-art-0df4a7d50de64cd6b3d104ab048ceccc |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-0df4a7d50de64cd6b3d104ab048ceccc2025-02-03T01:12:33ZengWileyGastroenterology Research and Practice1687-61211687-630X2018-01-01201810.1155/2018/96131859613185Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal CancerNaomi Yoshuantari0Didik Setyo Heriyanto1Susanna Hilda Hutajulu2Johan Kurnianda3Ahmad Ghozali4Department of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDivision of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDivision of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, IndonesiaBackground. Colorectal cancer (CRC) is both a global and national burden, being the third most common malignancy in men and the second in women, worldwide. The prognosis of CRC is affected by various factors like the histological grade, angiolymphatic invasion, and distant metastases. Metastasis is an intricate process; one of the possible mechanisms is through the interaction of the chemokines CXCL12 and CXCR4. This study aims to reveal the expression patterns of CXCL12 and CXCR4 in CRC. Methods. The quantitative expressions of CXCL12 and CXCR4 messenger RNA (mRNA) were evaluated in 32 patients with adenocarcinoma-type CRC. Real-time polymerase chain reaction (qRT-PCR) was performed on formalin-fixed tissues. CXCL12 and CXCR4’s expressions, clinicopathologic features, and the treatment response to the CRC were analysed. Results. All tumour tissues showed higher levels of both chemokines compared to normal colonic tissue. The expression of CXCL12 mRNA was higher in rectal location (p=0.04) with a tendency to be higher in later stages (p=0.15), while the expression of CXCR4 was lower in tumours with a lymphatic invasion (p=0.02), compared to their counterparts. There was no difference in the expression of CXCL12 and CXCR4 according to the patients’ ages, gender, tumour differentiation, or response to chemotherapy. Conclusion. Our study demonstrated that the mRNA expression of CXCL12 was significantly correlated with rectal location. CXCR4 mRNA expression was inversely correlated in tumours with a lymphatic invasion.http://dx.doi.org/10.1155/2018/9613185 |
| spellingShingle | Naomi Yoshuantari Didik Setyo Heriyanto Susanna Hilda Hutajulu Johan Kurnianda Ahmad Ghozali Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer Gastroenterology Research and Practice |
| title | Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer |
| title_full | Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer |
| title_fullStr | Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer |
| title_full_unstemmed | Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer |
| title_short | Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer |
| title_sort | clinicopathologic significance of cxcl12 and cxcr4 expressions in patients with colorectal cancer |
| url | http://dx.doi.org/10.1155/2018/9613185 |
| work_keys_str_mv | AT naomiyoshuantari clinicopathologicsignificanceofcxcl12andcxcr4expressionsinpatientswithcolorectalcancer AT didiksetyoheriyanto clinicopathologicsignificanceofcxcl12andcxcr4expressionsinpatientswithcolorectalcancer AT susannahildahutajulu clinicopathologicsignificanceofcxcl12andcxcr4expressionsinpatientswithcolorectalcancer AT johankurnianda clinicopathologicsignificanceofcxcl12andcxcr4expressionsinpatientswithcolorectalcancer AT ahmadghozali clinicopathologicsignificanceofcxcl12andcxcr4expressionsinpatientswithcolorectalcancer |