Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach

Abstract Despite critical roles in diseases, human pathways acting on strictly nuclear non-coding RNAs have been refractory to forward genetics. To enable their forward genetic discovery, we developed a single-cell approach that “Mirrors” activities of nuclear pathways with cytoplasmic fluorescence....

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Bibliographic Details
Main Authors: Rui Che, Monireh Panah, Bhoomi Mirani, Krista Knowles, Anastacia Ostapovich, Debarati Majumdar, Xiaotong Chen, Joseph DeSimone, William White, Megan Noonan, Hong Luo, Andrei Alexandrov
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59998-3
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