Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion
Kisspeptin–1 (KISS–1) and Spexin (SPX) are neuropeptides that play crucial roles in metabolism and sexual function, with their expression levels in tissues potentially influenced by antioxidant treatments. This study aimed to investigate the effects of cortexin treatment against ischemia–reperfusio...
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Universidad del Zulia
2025-04-01
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| Series: | Revista Científica |
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| Online Access: | https://produccioncientificaluz.org/index.php/cientifica/article/view/43814 |
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| author | Ahmet Turk Busra Zencirci Tuba Ozcan–Metin Ibrahim Bozgeyik Abdullah Karadag Seda Koçak |
| author_facet | Ahmet Turk Busra Zencirci Tuba Ozcan–Metin Ibrahim Bozgeyik Abdullah Karadag Seda Koçak |
| author_sort | Ahmet Turk |
| collection | DOAJ |
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Kisspeptin–1 (KISS–1) and Spexin (SPX) are neuropeptides that play crucial roles in metabolism and sexual function, with their expression levels in tissues potentially influenced by antioxidant treatments. This study aimed to investigate the effects of cortexin treatment against ischemia–reperfusion injury (I/R) resulting from testicular torsion on KISS–1 and SPX levels in testicular tissues. Twenty–eight male Sprague–Dawley, rats, aged 8–10 weeks, were divided into four equal groups: control, torsion, torsion/ detorsion, and torsion/detorsion+cortexin. At the conclusion of the experiment, histopathological and immunohistochemical analyses were performed to assess the expressions of KISS–1, SPX, tumor necrosis factor–alpha (TNF–α), and Caspase–3 in the testicular tissues. For biochemical analyses, total antioxidant status (TAS) and total oxidant status (TOS) levels were measured in serum samples using the ELISA method, while malondialdehyde (MDA) levels were assessed spectrophotometrically in testicular tissues. The results showed that compared to the control group, the torsion and torsion/detorsion groups exhibited significant histopathological damage, along with increased levels of MDA, TOS, Caspase–3, and TNF–α, and decreased levels of TAS, KISS–1, and SPX in the testicular tissues. Conversely, in the torsion+detorsion+cortexin group, which received treatment for reperfusion injury, there was a notable reduction in tissue damage, with decreased levels of MDA, TOS, caspase–3, and TNF–α, alongside increased levels of TAS, KISS, and SPX. Cortexin decreases testicular damage by reducing oxidative stress, increases spermatogenesis by improving seminiferous tubule and germinal epithelial thickness, and regulates KISS–1 and SPX expression, which have effects on the reproductive system.
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| format | Article |
| id | doaj-art-0dcab028c3ed4643892a3a2cf7b33dbc |
| institution | OA Journals |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-0dcab028c3ed4643892a3a2cf7b33dbc2025-08-20T02:20:12ZengUniversidad del ZuliaRevista Científica0798-22592521-97152025-04-0135210.52973/rcfcv-e35554Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsionAhmet Turk0Busra Zencirci1Tuba Ozcan–Metin2Ibrahim Bozgeyik3Abdullah Karadag4Seda Koçak5Adiyaman University, Faculty of Medicine, Department of Histology–Embryology. Adiyaman, Türkiye.Adiyaman University, Faculty of Medicine, Department of Anatomy. Adiyaman, Türkiye.Kahramanmaras Sutcu Imam University, Faculty of Medicine, Department of Histology and Embryology. Kahramanmaras, Türkiye.Adiyaman University, Faculty of Medicine, Department of Medical Biology. Adiyaman, Türkiye. Adiyaman University, Faculty of Medicine, Department of Physiology. Adiyaman, Türkiye. Kırşehir Ahi Evran University, Faculty of Medicine, Department of Physiology. Kırşehir, Türkiye. Kisspeptin–1 (KISS–1) and Spexin (SPX) are neuropeptides that play crucial roles in metabolism and sexual function, with their expression levels in tissues potentially influenced by antioxidant treatments. This study aimed to investigate the effects of cortexin treatment against ischemia–reperfusion injury (I/R) resulting from testicular torsion on KISS–1 and SPX levels in testicular tissues. Twenty–eight male Sprague–Dawley, rats, aged 8–10 weeks, were divided into four equal groups: control, torsion, torsion/ detorsion, and torsion/detorsion+cortexin. At the conclusion of the experiment, histopathological and immunohistochemical analyses were performed to assess the expressions of KISS–1, SPX, tumor necrosis factor–alpha (TNF–α), and Caspase–3 in the testicular tissues. For biochemical analyses, total antioxidant status (TAS) and total oxidant status (TOS) levels were measured in serum samples using the ELISA method, while malondialdehyde (MDA) levels were assessed spectrophotometrically in testicular tissues. The results showed that compared to the control group, the torsion and torsion/detorsion groups exhibited significant histopathological damage, along with increased levels of MDA, TOS, Caspase–3, and TNF–α, and decreased levels of TAS, KISS–1, and SPX in the testicular tissues. Conversely, in the torsion+detorsion+cortexin group, which received treatment for reperfusion injury, there was a notable reduction in tissue damage, with decreased levels of MDA, TOS, caspase–3, and TNF–α, alongside increased levels of TAS, KISS, and SPX. Cortexin decreases testicular damage by reducing oxidative stress, increases spermatogenesis by improving seminiferous tubule and germinal epithelial thickness, and regulates KISS–1 and SPX expression, which have effects on the reproductive system. https://produccioncientificaluz.org/index.php/cientifica/article/view/43814Kisspeptin–1SpexinTesticular damageTorsionDetorsion |
| spellingShingle | Ahmet Turk Busra Zencirci Tuba Ozcan–Metin Ibrahim Bozgeyik Abdullah Karadag Seda Koçak Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion Revista Científica Kisspeptin–1 Spexin Testicular damage Torsion Detorsion |
| title | Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion |
| title_full | Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion |
| title_fullStr | Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion |
| title_full_unstemmed | Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion |
| title_short | Effect of Cortexin administration on Kisspeptin and Spexin expression after testicular torsion |
| title_sort | effect of cortexin administration on kisspeptin and spexin expression after testicular torsion |
| topic | Kisspeptin–1 Spexin Testicular damage Torsion Detorsion |
| url | https://produccioncientificaluz.org/index.php/cientifica/article/view/43814 |
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