Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene
We present a systematic optimization and analysis of SPR biosensors, focusing on the influence of design parameters such as silver (Ag), silicon nitride (Si₃N₄), graphene, and ssDNA layer thicknesses. Two configurations, Sys₃ and Sys₅, were developed and numerically evaluated under varying SARS-CoV-...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000135 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864215258005504 |
|---|---|
| author | Talia Tene Diana Coello-Fiallos Myrian Borja Narcisa Sánchez Fabián Londo Cristian Vacacela Gomez Stefano Bellucci |
| author_facet | Talia Tene Diana Coello-Fiallos Myrian Borja Narcisa Sánchez Fabián Londo Cristian Vacacela Gomez Stefano Bellucci |
| author_sort | Talia Tene |
| collection | DOAJ |
| description | We present a systematic optimization and analysis of SPR biosensors, focusing on the influence of design parameters such as silver (Ag), silicon nitride (Si₃N₄), graphene, and ssDNA layer thicknesses. Two configurations, Sys₃ and Sys₅, were developed and numerically evaluated under varying SARS-CoV-2 concentrations in PBS solution, ranging from 0.01 nM to 100 nM. Sys₃, optimized with Ag at 55 nm, Si₃N₄ at 13 nm, and ssDNA at 10 nm, demonstrated exceptional sensitivity (371.7°/RIU), low limit of detection, and high detection accuracy, making it suitable for precision applications. In contrast, Sys₅, incorporating a graphene layer (0.34 nm) alongside Ag at 50 nm, Si₃N₄ at 10 nm, and ssDNA at 10 nm, exhibited superior robustness and a higher figure of merit (2287.2 RIU⁻1), offering consistent performance across a broader dynamic range. These results highlight the versatility of SPR biosensors in adapting to diverse diagnostic needs. |
| format | Article |
| id | doaj-art-0dc6e364756b468384d51e269cd88947 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-0dc6e364756b468384d51e269cd889472025-02-09T05:01:08ZengElsevierBiosensors and Bioelectronics: X2590-13702025-05-0123100586Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and grapheneTalia Tene0Diana Coello-Fiallos1Myrian Borja2Narcisa Sánchez3Fabián Londo4Cristian Vacacela Gomez5Stefano Bellucci6Department of Chemistry, Universidad Técnica Particular de Loja, 110160, Loja, Ecuador; Corresponding author.Surface Nanoscience Group, Department of Physics, University of Calabria, 87036, Rende, ItalyGrupo de Investigación Ciencia de Datos (CIDED), Escuela Superior Politécnica de Chimborazo (ESPOCH), Riobamba, 060155, EcuadorFacultad de Ciencias de la Educación, Humanas y Tecnologías, Universidad Nacional de Chimborazo (UNACH), Riobamba, 060110, EcuadorFacultad de Ciencias, Escuela Superior Politécnica de Chimborazo (ESPOCH), Riobamba, 060155, EcuadorINFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044, Frascati, Italy; Corresponding author.INFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044, Frascati, ItalyWe present a systematic optimization and analysis of SPR biosensors, focusing on the influence of design parameters such as silver (Ag), silicon nitride (Si₃N₄), graphene, and ssDNA layer thicknesses. Two configurations, Sys₃ and Sys₅, were developed and numerically evaluated under varying SARS-CoV-2 concentrations in PBS solution, ranging from 0.01 nM to 100 nM. Sys₃, optimized with Ag at 55 nm, Si₃N₄ at 13 nm, and ssDNA at 10 nm, demonstrated exceptional sensitivity (371.7°/RIU), low limit of detection, and high detection accuracy, making it suitable for precision applications. In contrast, Sys₅, incorporating a graphene layer (0.34 nm) alongside Ag at 50 nm, Si₃N₄ at 10 nm, and ssDNA at 10 nm, exhibited superior robustness and a higher figure of merit (2287.2 RIU⁻1), offering consistent performance across a broader dynamic range. These results highlight the versatility of SPR biosensors in adapting to diverse diagnostic needs.http://www.sciencedirect.com/science/article/pii/S2590137025000135SPR biosensorGrapheneSilicon nitrideSARS-CoV-2TMM approach |
| spellingShingle | Talia Tene Diana Coello-Fiallos Myrian Borja Narcisa Sánchez Fabián Londo Cristian Vacacela Gomez Stefano Bellucci Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene Biosensors and Bioelectronics: X SPR biosensor Graphene Silicon nitride SARS-CoV-2 TMM approach |
| title | Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene |
| title_full | Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene |
| title_fullStr | Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene |
| title_full_unstemmed | Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene |
| title_short | Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene |
| title_sort | surface plasmon resonance biosensors for sars cov 2 sensing the role of silicon nitride and graphene |
| topic | SPR biosensor Graphene Silicon nitride SARS-CoV-2 TMM approach |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000135 |
| work_keys_str_mv | AT taliatene surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT dianacoellofiallos surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT myrianborja surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT narcisasanchez surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT fabianlondo surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT cristianvacacelagomez surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene AT stefanobellucci surfaceplasmonresonancebiosensorsforsarscov2sensingtheroleofsiliconnitrideandgraphene |