Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products
Staphylococcus aureus is a known major cause of foodborne illnesses, and milk and dairy products are often contaminated by enterotoxigenic strains of this bacterium. In the present study, 122 S. aureus isolates collected from different dairy products were characterised by phenotypic properties, by t...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/501362 |
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| author | Stefano Morandi Milena Brasca Cristian Andrighetto Angiolella Lombardi Roberta Lodi |
| author_facet | Stefano Morandi Milena Brasca Cristian Andrighetto Angiolella Lombardi Roberta Lodi |
| author_sort | Stefano Morandi |
| collection | DOAJ |
| description | Staphylococcus aureus is a known major cause of foodborne illnesses, and milk and dairy products are often contaminated by enterotoxigenic strains of this bacterium. In the present study, 122 S. aureus isolates collected from different dairy products were characterised by phenotypic properties, by the distribution of genes encoding staphylococcal enterotoxins (sea, sec, sed, seg, seh, sei, sej, and sel) and by randomly amplified polymorphic DNA PCR (RAPD-PCR). Moreover, strain resistance to vancomycin and methicillin (oxacillin) was studied. The differences in the RAPD-PCR profiles obtained with the primers M13 and AP4 revealed the presence of a great genetic heterogeneity among the different S. aureus strains. Using the primer AP4 and M13, eight groups were distinguished by RAPD-PCR cluster analysis, although, except in few cases, it was not possible to correlate the isolates of different animal species (cow or ovine) with the presence of se genes. None of the isolates showed resistance to vancomycin or methicillin. |
| format | Article |
| id | doaj-art-0dc66aa0ac044145bf3380644ee8e907 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-0dc66aa0ac044145bf3380644ee8e9072025-02-03T01:07:58ZengWileyInternational Journal of Microbiology1687-918X1687-91982009-01-01200910.1155/2009/501362501362Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy ProductsStefano Morandi0Milena Brasca1Cristian Andrighetto2Angiolella Lombardi3Roberta Lodi4CNR Istituto di Scienze delle Produzioni Alimentari (ISPA), Sez. Milano, Via Celoria 2, 20133 Milan, ItalyCNR Istituto di Scienze delle Produzioni Alimentari (ISPA), Sez. Milano, Via Celoria 2, 20133 Milan, ItalyVeneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), ItalyVeneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), ItalyCNR Istituto di Scienze delle Produzioni Alimentari (ISPA), Sez. Milano, Via Celoria 2, 20133 Milan, ItalyStaphylococcus aureus is a known major cause of foodborne illnesses, and milk and dairy products are often contaminated by enterotoxigenic strains of this bacterium. In the present study, 122 S. aureus isolates collected from different dairy products were characterised by phenotypic properties, by the distribution of genes encoding staphylococcal enterotoxins (sea, sec, sed, seg, seh, sei, sej, and sel) and by randomly amplified polymorphic DNA PCR (RAPD-PCR). Moreover, strain resistance to vancomycin and methicillin (oxacillin) was studied. The differences in the RAPD-PCR profiles obtained with the primers M13 and AP4 revealed the presence of a great genetic heterogeneity among the different S. aureus strains. Using the primer AP4 and M13, eight groups were distinguished by RAPD-PCR cluster analysis, although, except in few cases, it was not possible to correlate the isolates of different animal species (cow or ovine) with the presence of se genes. None of the isolates showed resistance to vancomycin or methicillin.http://dx.doi.org/10.1155/2009/501362 |
| spellingShingle | Stefano Morandi Milena Brasca Cristian Andrighetto Angiolella Lombardi Roberta Lodi Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products International Journal of Microbiology |
| title | Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products |
| title_full | Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products |
| title_fullStr | Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products |
| title_full_unstemmed | Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products |
| title_short | Phenotypic and Genotypic Characterization of Staphylococcus aureus Strains from Italian Dairy Products |
| title_sort | phenotypic and genotypic characterization of staphylococcus aureus strains from italian dairy products |
| url | http://dx.doi.org/10.1155/2009/501362 |
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