Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations

Abstract The selection of IR spectroscopic approach for quantification of drugs supports green analytical chemistry principles without compromising the method analytical performance. The method follows pressed pellet technique for preparing samples using potassium bromide without using any toxic sol...

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Main Authors: Naga Prashant K, Suvarna Yenduri
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97485-3
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author Naga Prashant K
Suvarna Yenduri
author_facet Naga Prashant K
Suvarna Yenduri
author_sort Naga Prashant K
collection DOAJ
description Abstract The selection of IR spectroscopic approach for quantification of drugs supports green analytical chemistry principles without compromising the method analytical performance. The method follows pressed pellet technique for preparing samples using potassium bromide without using any toxic solvents and even reduced waste too. An easy, fast, and environmentally friendly FT-IR spectrophotometric method was developed and validated for the simultaneous measurement of amlodipine besylate (AML) and telmisartan (TEL) in pharmaceutical combination formulations. The absorbance spectra which was obtained from obtained transmittance spectra were used for determining area under curve and correlated with amount or concentration. AML and TEL shows peaks at 1206 cm− 1 and 863 cm− 1. Validation of the developed method followed ICH guidelines and all the validation results were within the limits. The LOD and LOQ of AML were found as 0.009359 %w/w, 0.028359 %w/w respectively where for TEL it was found to be 0.008241 %w/w and 0.024974 %w/w respectively. The MoGAPI, AGREE prep, and RGB model assessment procedure was green and sustainable. The described HPLC technique was not significantly different statistically.
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spelling doaj-art-0dbf86cce2a34da9911e7b27a45ce5942025-08-20T02:24:29ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-97485-3Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulationsNaga Prashant K0Suvarna Yenduri1Faculty of Pharmacy, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri UniversityFaculty of Pharmacy, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri UniversityAbstract The selection of IR spectroscopic approach for quantification of drugs supports green analytical chemistry principles without compromising the method analytical performance. The method follows pressed pellet technique for preparing samples using potassium bromide without using any toxic solvents and even reduced waste too. An easy, fast, and environmentally friendly FT-IR spectrophotometric method was developed and validated for the simultaneous measurement of amlodipine besylate (AML) and telmisartan (TEL) in pharmaceutical combination formulations. The absorbance spectra which was obtained from obtained transmittance spectra were used for determining area under curve and correlated with amount or concentration. AML and TEL shows peaks at 1206 cm− 1 and 863 cm− 1. Validation of the developed method followed ICH guidelines and all the validation results were within the limits. The LOD and LOQ of AML were found as 0.009359 %w/w, 0.028359 %w/w respectively where for TEL it was found to be 0.008241 %w/w and 0.024974 %w/w respectively. The MoGAPI, AGREE prep, and RGB model assessment procedure was green and sustainable. The described HPLC technique was not significantly different statistically.https://doi.org/10.1038/s41598-025-97485-3AmlodipineTelmisartanFTIR spectroscopyQuantitative analysisArea under curveEco-friendly
spellingShingle Naga Prashant K
Suvarna Yenduri
Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
Scientific Reports
Amlodipine
Telmisartan
FTIR spectroscopy
Quantitative analysis
Area under curve
Eco-friendly
title Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
title_full Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
title_fullStr Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
title_full_unstemmed Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
title_short Green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
title_sort green vibrational spectroscopic approach for simultaneous quantification of antihypertensive drugs in bulk and tablet formulations
topic Amlodipine
Telmisartan
FTIR spectroscopy
Quantitative analysis
Area under curve
Eco-friendly
url https://doi.org/10.1038/s41598-025-97485-3
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