Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells
Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting o...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2009-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000113023 |
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| _version_ | 1850152279626219520 |
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| author | Konstantin Matentzoglu Martin Scheffner |
| author_facet | Konstantin Matentzoglu Martin Scheffner |
| author_sort | Konstantin Matentzoglu |
| collection | DOAJ |
| description | Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest. |
| format | Article |
| id | doaj-art-0dba9515f1d1450790219338e03b0c8a |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-0dba9515f1d1450790219338e03b0c8a2025-08-20T02:26:02ZengTaylor & Francis GroupBioTechniques0736-62051940-98182009-01-01461212810.2144/000113023Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cellsKonstantin Matentzoglu0Martin Scheffner11University of Konstanz, Konstanz, Germany1University of Konstanz, Konstanz, GermanyClonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.https://www.future-science.com/doi/10.2144/000113023 |
| spellingShingle | Konstantin Matentzoglu Martin Scheffner Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells BioTechniques |
| title | Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells |
| title_full | Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells |
| title_fullStr | Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells |
| title_full_unstemmed | Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells |
| title_short | Ubiquitin-fusion protein system: a powerful tool for ectopic protein expression in mammalian cells |
| title_sort | ubiquitin fusion protein system a powerful tool for ectopic protein expression in mammalian cells |
| url | https://www.future-science.com/doi/10.2144/000113023 |
| work_keys_str_mv | AT konstantinmatentzoglu ubiquitinfusionproteinsystemapowerfultoolforectopicproteinexpressioninmammaliancells AT martinscheffner ubiquitinfusionproteinsystemapowerfultoolforectopicproteinexpressioninmammaliancells |