Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples
Growing concerns about the health risks of melamine adulteration in food products highlight the urgent need for reliable detection methods. However, the long-term effects of chronic low-level melamine exposure remain inadequately explored. This study introduces THE ONE InstantCare platform, a portab...
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| Format: | Article |
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MDPI AG
2024-11-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/12/12/870 |
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| author | Cheng-Hao Ko Wei-Yi Kong Abel Chernet Kabiso Wei-Huai Chiu Ashenafi Belihu Tadesse Chitsung Hong Chia-Fang Wu Hung-Hsun Lin |
| author_facet | Cheng-Hao Ko Wei-Yi Kong Abel Chernet Kabiso Wei-Huai Chiu Ashenafi Belihu Tadesse Chitsung Hong Chia-Fang Wu Hung-Hsun Lin |
| author_sort | Cheng-Hao Ko |
| collection | DOAJ |
| description | Growing concerns about the health risks of melamine adulteration in food products highlight the urgent need for reliable detection methods. However, the long-term effects of chronic low-level melamine exposure remain inadequately explored. This study introduces THE ONE InstantCare platform, a portable immunoassay analyzer integrating a SpectroChip-based spectral processing unit (SPU) with lateral flow immunoassay (LFIA) for sensitive and accurate quantification of melamine in human urine. This platform provides a cost-effective, rapid, and user-friendly point-of-care (POC) solution for melamine detection. Analytical evaluations across eight melamine concentrations (0–100 parts per billion, ppb) achieved a limit of detection (LOD) of 1.91 ppb. Validation with 24 human urine samples demonstrated strong concordance with liquid chromatography–mass spectrometry (LC-MS), yielding an intraclass correlation coefficient (ICC) of 0.9220, a Pearson correlation coefficient of 0.9389, and 95% agreement in Bland–Altman analysis. High reproducibility was observed, with an intraday coefficient of variation (CV) of 6.53% and acceptable interday CV values, while interference studies confirmed reliability in the presence of common biological substances. By delivering results in approximately 10 min, THE ONE InstantCare platform significantly reduces analysis time compared to LC-MS, which typically requires several hours. This novel platform enhances food safety surveillance and advances human health risk assessments, particularly for evaluating melamine-linked kidney damage. Its versatility and robust performance make it a promising tool for environmental monitoring and clinical diagnostics, enabling the detection of diverse biomarkers with high sensitivity and reproducibility. |
| format | Article |
| id | doaj-art-0db55d72414648e083ab1319e2004d5f |
| institution | DOAJ |
| issn | 2305-6304 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj-art-0db55d72414648e083ab1319e2004d5f2025-08-20T02:51:10ZengMDPI AGToxics2305-63042024-11-01121287010.3390/toxics12120870Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine SamplesCheng-Hao Ko0Wei-Yi Kong1Abel Chernet Kabiso2Wei-Huai Chiu3Ashenafi Belihu Tadesse4Chitsung Hong5Chia-Fang Wu6Hung-Hsun Lin7Graduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei 106, TaiwanGraduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei 106, TaiwanSpectroChip Inc., Hsinchu 310, TaiwanResearch Center for Environmental Changes, Academia Sinica (AS), Taipei 115, TaiwanResearch Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGrowing concerns about the health risks of melamine adulteration in food products highlight the urgent need for reliable detection methods. However, the long-term effects of chronic low-level melamine exposure remain inadequately explored. This study introduces THE ONE InstantCare platform, a portable immunoassay analyzer integrating a SpectroChip-based spectral processing unit (SPU) with lateral flow immunoassay (LFIA) for sensitive and accurate quantification of melamine in human urine. This platform provides a cost-effective, rapid, and user-friendly point-of-care (POC) solution for melamine detection. Analytical evaluations across eight melamine concentrations (0–100 parts per billion, ppb) achieved a limit of detection (LOD) of 1.91 ppb. Validation with 24 human urine samples demonstrated strong concordance with liquid chromatography–mass spectrometry (LC-MS), yielding an intraclass correlation coefficient (ICC) of 0.9220, a Pearson correlation coefficient of 0.9389, and 95% agreement in Bland–Altman analysis. High reproducibility was observed, with an intraday coefficient of variation (CV) of 6.53% and acceptable interday CV values, while interference studies confirmed reliability in the presence of common biological substances. By delivering results in approximately 10 min, THE ONE InstantCare platform significantly reduces analysis time compared to LC-MS, which typically requires several hours. This novel platform enhances food safety surveillance and advances human health risk assessments, particularly for evaluating melamine-linked kidney damage. Its versatility and robust performance make it a promising tool for environmental monitoring and clinical diagnostics, enabling the detection of diverse biomarkers with high sensitivity and reproducibility.https://www.mdpi.com/2305-6304/12/12/870melamine detectionSpectroChipspectral processing unit (SPU)point-of-care (POC)lateral flow immunoassay (LFIA)liquid chromatography–mass spectrometry (LC-MS) |
| spellingShingle | Cheng-Hao Ko Wei-Yi Kong Abel Chernet Kabiso Wei-Huai Chiu Ashenafi Belihu Tadesse Chitsung Hong Chia-Fang Wu Hung-Hsun Lin Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples Toxics melamine detection SpectroChip spectral processing unit (SPU) point-of-care (POC) lateral flow immunoassay (LFIA) liquid chromatography–mass spectrometry (LC-MS) |
| title | Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples |
| title_full | Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples |
| title_fullStr | Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples |
| title_full_unstemmed | Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples |
| title_short | Portable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples |
| title_sort | portable spectrochip based immunoassay platform for rapid and accurate melamine quantification in urine samples |
| topic | melamine detection SpectroChip spectral processing unit (SPU) point-of-care (POC) lateral flow immunoassay (LFIA) liquid chromatography–mass spectrometry (LC-MS) |
| url | https://www.mdpi.com/2305-6304/12/12/870 |
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