Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation
Soybean relies on symbiotic nitrogen fixation (SNF) to support sustainable agriculture. In this study, we conducted a comprehensive analysis of the GmMYB transcription factor subfamily 20, with a focus on <i>GmMYB62a</i> and <i>GmMYB62b</i>. Phylogenetic and structural analys...
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2025-07-01
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| author | Junchen Leng Ruobing Xu Yanshuang Liu Tianshu Jiang Haiying Hu Zhaojun Ding Shaojun Dai |
| author_facet | Junchen Leng Ruobing Xu Yanshuang Liu Tianshu Jiang Haiying Hu Zhaojun Ding Shaojun Dai |
| author_sort | Junchen Leng |
| collection | DOAJ |
| description | Soybean relies on symbiotic nitrogen fixation (SNF) to support sustainable agriculture. In this study, we conducted a comprehensive analysis of the GmMYB transcription factor subfamily 20, with a focus on <i>GmMYB62a</i> and <i>GmMYB62b</i>. Phylogenetic and structural analyses revealed that these genes are evolutionarily conserved among legumes and possess distinct domain architectures. Expression profiling and GUS staining showed that <i>GmMYB62a</i> and <i>GmMYB62b</i> are constitutively expressed in nodules. Functional analyses revealed that loss of <i>GmMYB62s</i> function significantly reduced nodule density, while overexpression promoted nodulation. Transcriptomic analysis (RNA-seq) further demonstrated that <i>GmMYB62s</i> regulate key pathways, including hormone signaling, immune responses, and cell wall metabolism, thereby coordinating symbiotic interactions. Collectively, our findings identify <i>GmMYB62a</i> and <i>GmMYB62b</i> as critical molecular regulators of nodulation in soybean, providing promising targets for improving symbiotic nitrogen fixation efficiency in legume crops. |
| format | Article |
| id | doaj-art-0db1659aa48e40d69ae129e6e2d6c48f |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-0db1659aa48e40d69ae129e6e2d6c48f2025-08-20T03:56:47ZengMDPI AGPlants2223-77472025-07-011414224010.3390/plants14142240Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean NodulationJunchen Leng0Ruobing Xu1Yanshuang Liu2Tianshu Jiang3Haiying Hu4Zhaojun Ding5Shaojun Dai6Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaSchool of Life Sciences, Shandong University, Qingdao 266228, ChinaDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaSchool of Life Sciences, Shandong University, Qingdao 266228, ChinaSchool of Life Sciences, Shandong University, Qingdao 266228, ChinaSchool of Life Sciences, Shandong University, Qingdao 266228, ChinaDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaSoybean relies on symbiotic nitrogen fixation (SNF) to support sustainable agriculture. In this study, we conducted a comprehensive analysis of the GmMYB transcription factor subfamily 20, with a focus on <i>GmMYB62a</i> and <i>GmMYB62b</i>. Phylogenetic and structural analyses revealed that these genes are evolutionarily conserved among legumes and possess distinct domain architectures. Expression profiling and GUS staining showed that <i>GmMYB62a</i> and <i>GmMYB62b</i> are constitutively expressed in nodules. Functional analyses revealed that loss of <i>GmMYB62s</i> function significantly reduced nodule density, while overexpression promoted nodulation. Transcriptomic analysis (RNA-seq) further demonstrated that <i>GmMYB62s</i> regulate key pathways, including hormone signaling, immune responses, and cell wall metabolism, thereby coordinating symbiotic interactions. Collectively, our findings identify <i>GmMYB62a</i> and <i>GmMYB62b</i> as critical molecular regulators of nodulation in soybean, providing promising targets for improving symbiotic nitrogen fixation efficiency in legume crops.https://www.mdpi.com/2223-7747/14/14/2240<i>GmMYB62a</i> and <i>GmMYB62b</i>transcription factorsoybeannodulation |
| spellingShingle | Junchen Leng Ruobing Xu Yanshuang Liu Tianshu Jiang Haiying Hu Zhaojun Ding Shaojun Dai Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation Plants <i>GmMYB62a</i> and <i>GmMYB62b</i> transcription factor soybean nodulation |
| title | Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation |
| title_full | Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation |
| title_fullStr | Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation |
| title_full_unstemmed | Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation |
| title_short | Genome-Wide Analysis of GmMYB S20 Transcription Factors Reveals Their Critical Role in Soybean Nodulation |
| title_sort | genome wide analysis of gmmyb s20 transcription factors reveals their critical role in soybean nodulation |
| topic | <i>GmMYB62a</i> and <i>GmMYB62b</i> transcription factor soybean nodulation |
| url | https://www.mdpi.com/2223-7747/14/14/2240 |
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