Regulation of DNA methylation in lesional tissue of children with atopic dermatitis
BackgroundGenetic and epigenetic mechanisms have been shown to play a role in the pathogenesis of atopic dermatitis (AD). However, the role of genes involved in the establishment of DNA methylation has not yet been demonstrated.MethodsA total of 15 pediatric patients with AD and 15 healthy volunteer...
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Frontiers Media S.A.
2025-03-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1531777/full |
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| author | Demet Kartal Muhammed Hanefi Dirican Serpil Taheri Mehmet Memiş Mehmet Memiş Eda Öksüm Solak Salih Levent Cinar Murat Borlu |
| author_facet | Demet Kartal Muhammed Hanefi Dirican Serpil Taheri Mehmet Memiş Mehmet Memiş Eda Öksüm Solak Salih Levent Cinar Murat Borlu |
| author_sort | Demet Kartal |
| collection | DOAJ |
| description | BackgroundGenetic and epigenetic mechanisms have been shown to play a role in the pathogenesis of atopic dermatitis (AD). However, the role of genes involved in the establishment of DNA methylation has not yet been demonstrated.MethodsA total of 15 pediatric patients with AD and 15 healthy volunteers were included in this study. The mRNA gene expression levels of eight different genes involved in the regulation of DNA methylation were examined in the blood and tissue samples.ResultsThe mRNA expression levels of DNMT3A genes were significantly increased, while the mRNA expression levels of DNMT3B, TET1, and TET2 genes were statistically significantly reduced in the lesional tissue of patients compared to the control group. It was observed that the mRNA expression levels of DNMT1, DNMT3A, and TET3 genes were increased, while the mRNA expression levels of DNMT3L and TET1 genes were found to be decreased in the blood of the patients.ConclusionThe results indicated that the DNA methylation pattern in the patients was hypermethylated, especially in the lesional tissue. The data obtained may contribute to the understanding of the epigenetic regulation of AD and aid in the development of new diagnostic and treatment options. |
| format | Article |
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| institution | OA Journals |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-0da66b879d004fdaaf5aeb85daabfaca2025-08-20T02:01:51ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-03-011210.3389/fmed.2025.15317771531777Regulation of DNA methylation in lesional tissue of children with atopic dermatitisDemet Kartal0Muhammed Hanefi Dirican1Serpil Taheri2Mehmet Memiş3Mehmet Memiş4Eda Öksüm Solak5Salih Levent Cinar6Murat Borlu7Dermatology and Venereology Department, Erciyes University Medical School, Kayseri, TürkiyeDermatology and Venereology Department, Tokat City Hospital, Tokat, TürkiyeBetul Ziya Eren Genome and Stem Cell (GENKÖK) Center, Erciyes University, Kayseri, TürkiyeBetul Ziya Eren Genome and Stem Cell (GENKÖK) Center, Erciyes University, Kayseri, TürkiyeTechnology Transfer Office Application and Research Center, Bayburt University, Bayburt, TürkiyeDermatology and Venereology Department, Erciyes University Medical School, Kayseri, TürkiyeDermatology and Venereology Department, Erciyes University Medical School, Kayseri, TürkiyeDermatology and Venereology Department, Erciyes University Medical School, Kayseri, TürkiyeBackgroundGenetic and epigenetic mechanisms have been shown to play a role in the pathogenesis of atopic dermatitis (AD). However, the role of genes involved in the establishment of DNA methylation has not yet been demonstrated.MethodsA total of 15 pediatric patients with AD and 15 healthy volunteers were included in this study. The mRNA gene expression levels of eight different genes involved in the regulation of DNA methylation were examined in the blood and tissue samples.ResultsThe mRNA expression levels of DNMT3A genes were significantly increased, while the mRNA expression levels of DNMT3B, TET1, and TET2 genes were statistically significantly reduced in the lesional tissue of patients compared to the control group. It was observed that the mRNA expression levels of DNMT1, DNMT3A, and TET3 genes were increased, while the mRNA expression levels of DNMT3L and TET1 genes were found to be decreased in the blood of the patients.ConclusionThe results indicated that the DNA methylation pattern in the patients was hypermethylated, especially in the lesional tissue. The data obtained may contribute to the understanding of the epigenetic regulation of AD and aid in the development of new diagnostic and treatment options.https://www.frontiersin.org/articles/10.3389/fmed.2025.1531777/fullatopic dermatitisDNA methylationTET1-2 and DNMT gene expressionepigeneticpediatric atopic dermatitis |
| spellingShingle | Demet Kartal Muhammed Hanefi Dirican Serpil Taheri Mehmet Memiş Mehmet Memiş Eda Öksüm Solak Salih Levent Cinar Murat Borlu Regulation of DNA methylation in lesional tissue of children with atopic dermatitis Frontiers in Medicine atopic dermatitis DNA methylation TET1-2 and DNMT gene expression epigenetic pediatric atopic dermatitis |
| title | Regulation of DNA methylation in lesional tissue of children with atopic dermatitis |
| title_full | Regulation of DNA methylation in lesional tissue of children with atopic dermatitis |
| title_fullStr | Regulation of DNA methylation in lesional tissue of children with atopic dermatitis |
| title_full_unstemmed | Regulation of DNA methylation in lesional tissue of children with atopic dermatitis |
| title_short | Regulation of DNA methylation in lesional tissue of children with atopic dermatitis |
| title_sort | regulation of dna methylation in lesional tissue of children with atopic dermatitis |
| topic | atopic dermatitis DNA methylation TET1-2 and DNMT gene expression epigenetic pediatric atopic dermatitis |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1531777/full |
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