Partial purification of protease from bovine spleen
The meat processing industry has significant to food production and economy in Mongolia. According to the National Statistical Commission, more than 9423 tons of liver, 2011 tons of kidney, 3121 tons of heart and 1361 tons of spleen are being wasted annually at slaughterhouses. These enzymes have va...
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| Format: | Article |
| Language: | English |
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National University of Mongolia
2023-12-01
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| Series: | Mongolian Journal of Biological Sciences |
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| Online Access: | https://www.biotaxa.org/mjbs/article/view/83658 |
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| author | Bayarmaa Jambalsuren Purev Dondog Punsaldulam Dashnyam Munkhgerel Khurelchuluun |
| author_facet | Bayarmaa Jambalsuren Purev Dondog Punsaldulam Dashnyam Munkhgerel Khurelchuluun |
| author_sort | Bayarmaa Jambalsuren |
| collection | DOAJ |
| description | The meat processing industry has significant to food production and economy in Mongolia. According to the National Statistical Commission, more than 9423 tons of liver, 2011 tons of kidney, 3121 tons of heart and 1361 tons of spleen are being wasted annually at slaughterhouses. These enzymes have various applications in different industries, including food processing, pharmaceuticals, and biotechnology research. Several studies have been conducted on the isolation and characterization of trypsin and chymotrypsin from bovine and caprine pancreas in the 1980s. Currently, there is no research on animal spleen enzymes has been reported in Mongolia. In this study, we outlined the methods for partial purification of proteases from bovine spleen. The process involved two steps: precipitation (ammonium sulfate or acetone) followed with dialysis and ion exchange chromatography (DEAE-Cellulose). In the final step, the specific activity of the isolated proteolytic fraction was shown to be 680.45 U, it’s activity improved by 545.58-fold in relative to the initial spleen extract. Approximate molecular weight of the purified protease was 28 kDa as determined by SDS-PAGE. |
| format | Article |
| id | doaj-art-0da393b49ccb4ab89f1e988da599e2ac |
| institution | DOAJ |
| issn | 1684-3908 2225-4994 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | National University of Mongolia |
| record_format | Article |
| series | Mongolian Journal of Biological Sciences |
| spelling | doaj-art-0da393b49ccb4ab89f1e988da599e2ac2025-08-20T03:22:26ZengNational University of MongoliaMongolian Journal of Biological Sciences1684-39082225-49942023-12-01212152010.22353/mjbs.2023.21.12Partial purification of protease from bovine spleenBayarmaa Jambalsuren0Purev Dondog1Punsaldulam Dashnyam2Munkhgerel Khurelchuluun3Department of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14201, MongoliaDepartment of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14201, MongoliaLaboratory of Microbial Synthesis, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar 13330, Mongolia Department of Bio-engineering, Mongol Koosen College of Technology, Ulaanbaatar 16081, MongoliaThe meat processing industry has significant to food production and economy in Mongolia. According to the National Statistical Commission, more than 9423 tons of liver, 2011 tons of kidney, 3121 tons of heart and 1361 tons of spleen are being wasted annually at slaughterhouses. These enzymes have various applications in different industries, including food processing, pharmaceuticals, and biotechnology research. Several studies have been conducted on the isolation and characterization of trypsin and chymotrypsin from bovine and caprine pancreas in the 1980s. Currently, there is no research on animal spleen enzymes has been reported in Mongolia. In this study, we outlined the methods for partial purification of proteases from bovine spleen. The process involved two steps: precipitation (ammonium sulfate or acetone) followed with dialysis and ion exchange chromatography (DEAE-Cellulose). In the final step, the specific activity of the isolated proteolytic fraction was shown to be 680.45 U, it’s activity improved by 545.58-fold in relative to the initial spleen extract. Approximate molecular weight of the purified protease was 28 kDa as determined by SDS-PAGE.https://www.biotaxa.org/mjbs/article/view/83658bovine spleenproteolytic enzymepurification |
| spellingShingle | Bayarmaa Jambalsuren Purev Dondog Punsaldulam Dashnyam Munkhgerel Khurelchuluun Partial purification of protease from bovine spleen Mongolian Journal of Biological Sciences bovine spleen proteolytic enzyme purification |
| title | Partial purification of protease from bovine spleen |
| title_full | Partial purification of protease from bovine spleen |
| title_fullStr | Partial purification of protease from bovine spleen |
| title_full_unstemmed | Partial purification of protease from bovine spleen |
| title_short | Partial purification of protease from bovine spleen |
| title_sort | partial purification of protease from bovine spleen |
| topic | bovine spleen proteolytic enzyme purification |
| url | https://www.biotaxa.org/mjbs/article/view/83658 |
| work_keys_str_mv | AT bayarmaajambalsuren partialpurificationofproteasefrombovinespleen AT purevdondog partialpurificationofproteasefrombovinespleen AT punsaldulamdashnyam partialpurificationofproteasefrombovinespleen AT munkhgerelkhurelchuluun partialpurificationofproteasefrombovinespleen |