Partial purification of protease from bovine spleen
The meat processing industry has significant to food production and economy in Mongolia. According to the National Statistical Commission, more than 9423 tons of liver, 2011 tons of kidney, 3121 tons of heart and 1361 tons of spleen are being wasted annually at slaughterhouses. These enzymes have va...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
National University of Mongolia
2023-12-01
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| Series: | Mongolian Journal of Biological Sciences |
| Subjects: | |
| Online Access: | https://www.biotaxa.org/mjbs/article/view/83658 |
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| Summary: | The meat processing industry has significant to food production and economy in Mongolia. According to the National Statistical Commission, more than 9423 tons of liver, 2011 tons of kidney, 3121 tons of heart and 1361 tons of spleen are being wasted annually at slaughterhouses. These enzymes have various applications in different industries, including food processing, pharmaceuticals, and biotechnology research. Several studies have been conducted on the isolation and characterization of trypsin and chymotrypsin from bovine and caprine pancreas in the 1980s. Currently, there is no research on animal spleen enzymes has been reported in Mongolia. In this study, we outlined the methods for partial purification of proteases from bovine spleen. The process involved two steps: precipitation (ammonium sulfate or acetone) followed with dialysis and ion exchange chromatography (DEAE-Cellulose). In the final step, the specific activity of the isolated proteolytic fraction was shown to be 680.45 U, it’s activity improved by 545.58-fold in relative to the initial spleen extract. Approximate molecular weight of the purified protease was 28 kDa as determined by SDS-PAGE. |
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| ISSN: | 1684-3908 2225-4994 |