Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
Summary: Protein synthesis could control spermatogenic cell fate transitions. Here, we present a protocol for visualization and quantification of newly synthesized proteins by click-chemistry-based immunofluorescence within specific spermatogenic cell types in the mice testicular lumen. We detail th...
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| Main Authors: | Dingfeng Zou, Kai Li, Yan Lu, Wei Yan, Wei Song |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500067X |
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