Serum microRNAs as peripheral markers of primary aldosteronism
BackgroundPrimary aldosteronism (PA) is the principal cause of secondary hypertension; it leads to significantly elevated cardiovascular morbidity and mortality, but only a fraction of its cases ever get detected, partially due to diagnostic procedures that are difficult to perform and to interpret....
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2025.1511096/full |
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| author | Nikita Makhnov Nikita Makhnov Nikita Makhnov Fredrik Axling Elham Barazeghi Peter Stålberg Tobias Åkerström Tobias Åkerström Per Hellman Per Hellman |
| author_facet | Nikita Makhnov Nikita Makhnov Nikita Makhnov Fredrik Axling Elham Barazeghi Peter Stålberg Tobias Åkerström Tobias Åkerström Per Hellman Per Hellman |
| author_sort | Nikita Makhnov |
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| description | BackgroundPrimary aldosteronism (PA) is the principal cause of secondary hypertension; it leads to significantly elevated cardiovascular morbidity and mortality, but only a fraction of its cases ever get detected, partially due to diagnostic procedures that are difficult to perform and to interpret. More straightforward diagnostic methods are needed. Lateralized, or unilateral PA (uPA), is best treated by surgery. Bilateral PA (bPA) is treated medically.AimThe aim of our study was to explore microRNA (miRNA) in peripheral blood as markers of PA, uPA and bPA.MethodsIn groups of subjects with primary hypertension (HT, n = 11), bPA (n = 12), and uPA (n = 16), peripheral serum was used for isolation of total RNA, library preparation, and NGS sequencing to achieve a comparative analysis of miRNA expression. Five-fold cross-validation support vector machine learning (ML) models were employed to search for miRNA that could be used as markers of PA and its forms.ResultsIn our cohort of patients, the discovered combinations of miRNAs could, with a high level of accuracy, sensitivity, and specificity, characterize the difference between HT and PA, as well as between a combined group of HT + bPA vs. uPA. The differentiating parameters were moderately good for comparison of bPA vs. uPA.ConclusionWithin our patient cohort, and using ML, the study identified distinctly different miRNA profiles between HT and PA, as well as between bPA and uPA. Further validation studies may lead to the emergence of a new tool for clinical diagnostics of PA. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj-art-0d9b950f2f734155a7ea9442e1de8cb32025-08-20T03:39:49ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-03-011610.3389/fendo.2025.15110961511096Serum microRNAs as peripheral markers of primary aldosteronismNikita Makhnov0Nikita Makhnov1Nikita Makhnov2Fredrik Axling3Elham Barazeghi4Peter Stålberg5Tobias Åkerström6Tobias Åkerström7Per Hellman8Per Hellman9Department of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgery, Karlstad Central Hospital, Karlstad, SwedenCenter for Clinical Research and Education, Region of Värmland, Karlstad, SwedenDepartment of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgery, Uppsala University Hospital, Uppsala, SwedenDepartment of Surgical Sciences, Uppsala University, Uppsala, SwedenDepartment of Surgery, Uppsala University Hospital, Uppsala, SwedenBackgroundPrimary aldosteronism (PA) is the principal cause of secondary hypertension; it leads to significantly elevated cardiovascular morbidity and mortality, but only a fraction of its cases ever get detected, partially due to diagnostic procedures that are difficult to perform and to interpret. More straightforward diagnostic methods are needed. Lateralized, or unilateral PA (uPA), is best treated by surgery. Bilateral PA (bPA) is treated medically.AimThe aim of our study was to explore microRNA (miRNA) in peripheral blood as markers of PA, uPA and bPA.MethodsIn groups of subjects with primary hypertension (HT, n = 11), bPA (n = 12), and uPA (n = 16), peripheral serum was used for isolation of total RNA, library preparation, and NGS sequencing to achieve a comparative analysis of miRNA expression. Five-fold cross-validation support vector machine learning (ML) models were employed to search for miRNA that could be used as markers of PA and its forms.ResultsIn our cohort of patients, the discovered combinations of miRNAs could, with a high level of accuracy, sensitivity, and specificity, characterize the difference between HT and PA, as well as between a combined group of HT + bPA vs. uPA. The differentiating parameters were moderately good for comparison of bPA vs. uPA.ConclusionWithin our patient cohort, and using ML, the study identified distinctly different miRNA profiles between HT and PA, as well as between bPA and uPA. Further validation studies may lead to the emergence of a new tool for clinical diagnostics of PA.https://www.frontiersin.org/articles/10.3389/fendo.2025.1511096/fullhyperaldosteronismhypertensionmicroRNAsgenetic markersmachine learningserum |
| spellingShingle | Nikita Makhnov Nikita Makhnov Nikita Makhnov Fredrik Axling Elham Barazeghi Peter Stålberg Tobias Åkerström Tobias Åkerström Per Hellman Per Hellman Serum microRNAs as peripheral markers of primary aldosteronism Frontiers in Endocrinology hyperaldosteronism hypertension microRNAs genetic markers machine learning serum |
| title | Serum microRNAs as peripheral markers of primary aldosteronism |
| title_full | Serum microRNAs as peripheral markers of primary aldosteronism |
| title_fullStr | Serum microRNAs as peripheral markers of primary aldosteronism |
| title_full_unstemmed | Serum microRNAs as peripheral markers of primary aldosteronism |
| title_short | Serum microRNAs as peripheral markers of primary aldosteronism |
| title_sort | serum micrornas as peripheral markers of primary aldosteronism |
| topic | hyperaldosteronism hypertension microRNAs genetic markers machine learning serum |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1511096/full |
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