Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed
The main disadvantages of using soybean oil extraction waste as a raw feed material are its high contents of fiber, fat, and anti-nutritional factors. Therefore, several processing methods such as extrusion and hydrolysis are used to overcome these disadvantages and increase the availability of high...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/4/209 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850144115665141760 |
|---|---|
| author | Marina Belyshkina Tamara Kobozeva Mikhail Zagoruiko Oksana Serebryakova Maisoon Shaaban Tatiana Ananeva Igor Bashmakov |
| author_facet | Marina Belyshkina Tamara Kobozeva Mikhail Zagoruiko Oksana Serebryakova Maisoon Shaaban Tatiana Ananeva Igor Bashmakov |
| author_sort | Marina Belyshkina |
| collection | DOAJ |
| description | The main disadvantages of using soybean oil extraction waste as a raw feed material are its high contents of fiber, fat, and anti-nutritional factors. Therefore, several processing methods such as extrusion and hydrolysis are used to overcome these disadvantages and increase the availability of high-quality proteins to animals from this by-product. This study is concerned with the hydrolysis of extruded soybean meal in the presence of bacterial alkaline proteases. The effects of various process parameters were investigated to determine the optimal process parameters for hydrolysis in terms of the total free amino acid and amine nitrogen contents. The experiment included two sets of parameters that were selected for comparison: the temperature and pH in ranges of t 45–50 °C, pH 8–11, compared to the temperature and pH ranges of t = 40–45 °C and pH 7–9, using three enzyme/substrate ratios (1:10, 1:20, and 1:30). The protein hydrolysate was stored for three months after it was treated with two different preservatives (sorbic acid and thymol). Based on the results, it was found that the total free amino acid content was higher when the temperature range was 45–50 °C, the pH range was 8–11, and sorbic acid was used as a preservative. |
| format | Article |
| id | doaj-art-0d897b8cf0f540ca8e9d78c4a25d97ba |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-0d897b8cf0f540ca8e9d78c4a25d97ba2025-08-20T02:28:28ZengMDPI AGFermentation2311-56372025-04-0111420910.3390/fermentation11040209Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal FeedMarina Belyshkina0Tamara Kobozeva1Mikhail Zagoruiko2Oksana Serebryakova3Maisoon Shaaban4Tatiana Ananeva5Igor Bashmakov6Federal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 5. 1st Institutskiy Proezd Str., Moscow 109428, RussiaThe main disadvantages of using soybean oil extraction waste as a raw feed material are its high contents of fiber, fat, and anti-nutritional factors. Therefore, several processing methods such as extrusion and hydrolysis are used to overcome these disadvantages and increase the availability of high-quality proteins to animals from this by-product. This study is concerned with the hydrolysis of extruded soybean meal in the presence of bacterial alkaline proteases. The effects of various process parameters were investigated to determine the optimal process parameters for hydrolysis in terms of the total free amino acid and amine nitrogen contents. The experiment included two sets of parameters that were selected for comparison: the temperature and pH in ranges of t 45–50 °C, pH 8–11, compared to the temperature and pH ranges of t = 40–45 °C and pH 7–9, using three enzyme/substrate ratios (1:10, 1:20, and 1:30). The protein hydrolysate was stored for three months after it was treated with two different preservatives (sorbic acid and thymol). Based on the results, it was found that the total free amino acid content was higher when the temperature range was 45–50 °C, the pH range was 8–11, and sorbic acid was used as a preservative.https://www.mdpi.com/2311-5637/11/4/209amine nitrogenfree amino acidsprotein hydrolysatehydrolysate storagethymolsorbic acid |
| spellingShingle | Marina Belyshkina Tamara Kobozeva Mikhail Zagoruiko Oksana Serebryakova Maisoon Shaaban Tatiana Ananeva Igor Bashmakov Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed Fermentation amine nitrogen free amino acids protein hydrolysate hydrolysate storage thymol sorbic acid |
| title | Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed |
| title_full | Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed |
| title_fullStr | Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed |
| title_full_unstemmed | Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed |
| title_short | Determination of the Optimal Biotechnological Parameters for Industrial Production of Protein Hydrolysates for Animal Feed |
| title_sort | determination of the optimal biotechnological parameters for industrial production of protein hydrolysates for animal feed |
| topic | amine nitrogen free amino acids protein hydrolysate hydrolysate storage thymol sorbic acid |
| url | https://www.mdpi.com/2311-5637/11/4/209 |
| work_keys_str_mv | AT marinabelyshkina determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT tamarakobozeva determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT mikhailzagoruiko determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT oksanaserebryakova determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT maisoonshaaban determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT tatianaananeva determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed AT igorbashmakov determinationoftheoptimalbiotechnologicalparametersforindustrialproductionofproteinhydrolysatesforanimalfeed |